Correlation between controlled lung collapse and early lung injury in dogs

Liu,Gaowang; Wang,Hongyan; Lu,Xin; Ma,Xianfeng; Xiao,Ming; Xiao,Pan; Wei,Yan; Yang,Miao; Yang,Xueying; Yan,Xiao; Zhang,Aixing; Li,Rui; Tang,Jianjun; Liu,Xiaojun; Zhang,Yating; Xiao,Jinfang

doi:10.3892/etm.2018.6531

October-2018 Volume 16 Issue 4

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October-2018 Volume 16 Issue 4

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Article Open Access

Correlation between controlled lung collapse and early lung injury in dogs

Authors:
- Gaowang Liu
- Hongyan Wang
- Xin Lu
- Xianfeng Ma
- Ming Xiao
- Pan Xiao
- Yan Wei
- Miao Yang
- Xueying Yang
- Xiao Yan
- Aixing Zhang
- Rui Li
- Jianjun Tang
- Xiaojun Liu
- Yating Zhang
- Jinfang Xiao
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Affiliations: Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Cardiovascular Medicine, Xian Tao Hospital of Yangtze University, Wuhan, Hubei 433013, P.R. China, Sino-French Institute of Nuclear Engineering and Technology, Sun Yat-Sen University, Zhuhai, Guangdong 519082, P.R. China, Department of ENT, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China, Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510515, P.R. China
Pages: 3027-3033
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Published online on: July 27, 2018

https://doi.org/10.3892/etm.2018.6531
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Abstract

A new type of pulmonary sequestration ventilator was used to compare the relationship between controlled lung collapse and early lung injury in thoracic surgery for dogs. Eighteen experimental dogs were randomly divided into three groups (G1-G3 groups). After general anesthesia, the shunt balance in lung was controlled and the pulmonary sequestration tube was placed in the femoral artery and vein, and the Swan-Ganz tube was placed into the right internal jugular vein as well. Two-lung ventilation (TLV) was first performed for 20 min, followed by one-lung ventilation (OLV). The degree of collapse was 100% (G1), 90% (G2), and 50% (G3). Blood samples were extracted from femoral artery and jugular vein prior to collapse (T0), and at 30 (T1), 60 (T2), and 120 (T3) min after collapse for blood gas analysis to determine the shunt ratio (Qs/Qt). Blood samples were also subjected to enzyme linked immunosorbent assay (ELISA) to determine serum tumor necrosis factor-α (TNF-α), intercellular immune adhesion molecule-1 (ICAM-1) and interleukin-6 (IL-6) levels. Arterial blood pressure, heart rate, pulmonary artery pressure and other physiological indicators were monitored during the experiment. Lung tissues were collected at T3 to calculate the wet/dry weight ratio (W/D). Histopathological changes were observed and compared by microscopic observation and blind scoring of pathological section after hematoxylin and eosin (H&E) staining. There were no significant differences in the physiological indexes between the two groups during TLV (P>0.05). Mean pulmonary arterial pressure (MPAP) in G2 and G3 groups was significantly more stable than that in G1 group after OLV (P<0.05); shunt ratio Qs/Qt, W/D, and serum TNF-α, ICAM-1 and IL-6 levels in the lung were decreased; and the degrees of pulmonary edema, hemorrhage, inflammatory cell infiltration and lung injury were also decreased. There was no statistically significant difference in each index at each time-point between G2 and G3 groups (P>0.05). Compared with complete lung collapse (collapse degree: 100%), controlled lung collapse (collapse degree: 90% and 50%) can better reduce the intraoperative lung injury, but there was no significant difference between the collapse degrees of 90 and 50%.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Correlation between controlled lung collapse and early lung injury in dogs

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