Regulation of mPGES‑1 composition and cell growth via the MAPK signaling pathway in jurkat cells

  • Authors:
    • Yi‑Qing Li
    • Jiao‑Ting Chen
    • Song‑Mei Yin
    • Da‑Nian Nie
    • Zhi‑Yuan He
    • Shuang‑Feng Xie
    • Xiu‑Ju Wang
    • Yu‑Dan Wu
    • Jie Xiao
    • Hong‑Yun Liu
    • Jie‑Yu Wang
    • Wen‑Juan Yang
    • Li‑Ping Ma
  • View Affiliations

  • Published online on: July 30, 2018     https://doi.org/10.3892/etm.2018.6538
  • Pages: 3211-3219
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Abstract

Previous studies have suggested that microsomal prostaglandin E synthase‑1 (mPGES‑1) is highly expressed and closely associated with mitogen‑activated protein kinase (MAPK) signaling pathways in various types of malignant cells. However, their expression patterns and function with respect to T‑cell acute lymphoblastic leukemia (T‑ALL) remain largely unknown. The present study investigated whether mPGES‑1 served a crucial role in T‑ALL and aimed to identify interactions between mPGES‑1 and the MAPK signaling pathway in T‑ALL. The results indicated that mPGES‑1 overexpression in T‑ALL jurkat cells was significantly decreased by RNA silencing. Decreasing mPGES‑1 on a consistent basis may inhibit cell proliferation, induce apoptosis and arrest the cell cycle in T‑ALL jurkat cells. Microarray and western blot analyses revealed that c‑Jun N‑terminal kinase served a role in the mPGES‑1/prostaglandin E2/EP4/MAPK positive feedback loops. In addition, P38 and extracellular signal‑regulated kinase 1/2 exhibited negative feedback effects on mPGES‑1. In conclusion, the results suggested that cross‑talk between mPGES‑1 and the MAPK signaling pathway was very complex. Therefore, the combined regulation of mPGES‑1 and the MAPK signaling pathway may be developed into a new candidate therapy for T‑ALL in the future.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li YQ, Chen JT, Yin SM, Nie DN, He ZY, Xie SF, Wang XJ, Wu YD, Xiao J, Liu HY, Liu HY, et al: Regulation of mPGES‑1 composition and cell growth via the MAPK signaling pathway in jurkat cells. Exp Ther Med 16: 3211-3219, 2018
APA
Li, Y., Chen, J., Yin, S., Nie, D., He, Z., Xie, S. ... Ma, L. (2018). Regulation of mPGES‑1 composition and cell growth via the MAPK signaling pathway in jurkat cells. Experimental and Therapeutic Medicine, 16, 3211-3219. https://doi.org/10.3892/etm.2018.6538
MLA
Li, Y., Chen, J., Yin, S., Nie, D., He, Z., Xie, S., Wang, X., Wu, Y., Xiao, J., Liu, H., Wang, J., Yang, W., Ma, L."Regulation of mPGES‑1 composition and cell growth via the MAPK signaling pathway in jurkat cells". Experimental and Therapeutic Medicine 16.4 (2018): 3211-3219.
Chicago
Li, Y., Chen, J., Yin, S., Nie, D., He, Z., Xie, S., Wang, X., Wu, Y., Xiao, J., Liu, H., Wang, J., Yang, W., Ma, L."Regulation of mPGES‑1 composition and cell growth via the MAPK signaling pathway in jurkat cells". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3211-3219. https://doi.org/10.3892/etm.2018.6538