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Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics

  • Authors:
    • Xing Xue
    • Ying Lv
    • Yufang Leng
    • Yan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Resource and Environmental Engineering, Gansu Agricultural University, Lanzhou, Gansu 730070, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3011-3019
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    Published online on: July 30, 2018
       https://doi.org/10.3892/etm.2018.6540
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Abstract

The neurotoxicity of local anesthetics has received an increasing amount of attention and more effective therapeutic agents are required. Extracellular polymeric substances from Aphanizomenon flos‑aquae (EPS‑A) are high molecular weight polysaccharides. The present study aimed to elucidate the protective effects of EPS‑A on neurotoxicity induced by local anesthetics in an intraperitoneal injection bupivacaine rat model. The results of immunohistochemical staining inicated that following intraperitoneal injection of EPS‑A the levels of apoptosis and caspase‑3 decreased, and the expression levels of microtubule‑associated protein 1A light chain 3 (LC3) and beclin1 increased. In order to further clarify the mechanism of the EPS‑A‑mediated protection, the expression of key proteins associated with autophagy was investigated by western blotting. The results suggested that the ratio of LC3‑II/LC3‑I and the expression level of beclin1 increased. Taken together, the results indicated that EPS‑A induced neuroprotective effects on bupivacaine‑induced neurotoxicity. The underlying mechanism may be associated with the inhibition of apoptosis, upregulation of autophagy and improvement of cell survival. The results suggested that EPS‑A may be a candidate neuroprotective agent against neurotoxicity caused by local anesthetics.
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Copy and paste a formatted citation
Spandidos Publications style
Xue X, Lv Y, Leng Y and Zhang Y: Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics. Exp Ther Med 16: 3011-3019, 2018.
APA
Xue, X., Lv, Y., Leng, Y., & Zhang, Y. (2018). Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics. Experimental and Therapeutic Medicine, 16, 3011-3019. https://doi.org/10.3892/etm.2018.6540
MLA
Xue, X., Lv, Y., Leng, Y., Zhang, Y."Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics". Experimental and Therapeutic Medicine 16.4 (2018): 3011-3019.
Chicago
Xue, X., Lv, Y., Leng, Y., Zhang, Y."Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3011-3019. https://doi.org/10.3892/etm.2018.6540
Copy and paste a formatted citation
x
Spandidos Publications style
Xue X, Lv Y, Leng Y and Zhang Y: Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics. Exp Ther Med 16: 3011-3019, 2018.
APA
Xue, X., Lv, Y., Leng, Y., & Zhang, Y. (2018). Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics. Experimental and Therapeutic Medicine, 16, 3011-3019. https://doi.org/10.3892/etm.2018.6540
MLA
Xue, X., Lv, Y., Leng, Y., Zhang, Y."Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics". Experimental and Therapeutic Medicine 16.4 (2018): 3011-3019.
Chicago
Xue, X., Lv, Y., Leng, Y., Zhang, Y."Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3011-3019. https://doi.org/10.3892/etm.2018.6540
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