Open Access

Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics

  • Authors:
    • Xing Xue
    • Ying Lv
    • Yufang Leng
    • Yan Zhang
  • View Affiliations

  • Published online on: July 30, 2018     https://doi.org/10.3892/etm.2018.6540
  • Pages: 3011-3019
  • Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The neurotoxicity of local anesthetics has received an increasing amount of attention and more effective therapeutic agents are required. Extracellular polymeric substances from Aphanizomenon flos‑aquae (EPS‑A) are high molecular weight polysaccharides. The present study aimed to elucidate the protective effects of EPS‑A on neurotoxicity induced by local anesthetics in an intraperitoneal injection bupivacaine rat model. The results of immunohistochemical staining inicated that following intraperitoneal injection of EPS‑A the levels of apoptosis and caspase‑3 decreased, and the expression levels of microtubule‑associated protein 1A light chain 3 (LC3) and beclin1 increased. In order to further clarify the mechanism of the EPS‑A‑mediated protection, the expression of key proteins associated with autophagy was investigated by western blotting. The results suggested that the ratio of LC3‑II/LC3‑I and the expression level of beclin1 increased. Taken together, the results indicated that EPS‑A induced neuroprotective effects on bupivacaine‑induced neurotoxicity. The underlying mechanism may be associated with the inhibition of apoptosis, upregulation of autophagy and improvement of cell survival. The results suggested that EPS‑A may be a candidate neuroprotective agent against neurotoxicity caused by local anesthetics.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xue X, Lv Y, Leng Y and Zhang Y: Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics. Exp Ther Med 16: 3011-3019, 2018
APA
Xue, X., Lv, Y., Leng, Y., & Zhang, Y. (2018). Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics. Experimental and Therapeutic Medicine, 16, 3011-3019. https://doi.org/10.3892/etm.2018.6540
MLA
Xue, X., Lv, Y., Leng, Y., Zhang, Y."Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics". Experimental and Therapeutic Medicine 16.4 (2018): 3011-3019.
Chicago
Xue, X., Lv, Y., Leng, Y., Zhang, Y."Protective effects of extracellular polymeric substances from Aphanizomenon flos‑aquae on neurotoxicity induced by local anesthetics". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3011-3019. https://doi.org/10.3892/etm.2018.6540