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Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects

  • Authors:
    • Hao Sun
    • Yue Huang
    • Lei Zhang
    • Biao Li
    • Xudong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Cranio‑Maxillofacial Surgery, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P.R. China, Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2969-2977
    |
    Published online on: August 1, 2018
       https://doi.org/10.3892/etm.2018.6551
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Abstract

This study explored the feasibility of inducing the differentiation of BMSCs into chondrocytes through co‑culture with chondrocytes in hydrogel constructs (Pluronic F‑127 gel) in vivo for the repair of goat mandibular condylar cartilage defects. Chondrocytes and BMSCs were isolated from goat auricular cartilage and bone marrow, respectively, and were mixed at a ratio of 3:7. BMSCs were labelled with green fluorescence protein (GFP) using a retrovirus vector for tracing. Mixed cells were re‑suspended in 30% Pluronic F‑127 at a concentration of 5x107 cells/ml to form a gel‑cell complex. The gel‑cell complex was implanted into the temporomandibular joint condylar articular cartilage defects. The whole temporomandibular joint and adjacent tissues were harvested at 4, 8, and 12 weeks after surgery, and gross observation, histology and collagen II expression were evaluated. In the co‑culture group, cartilage‑like tissues were formed, and abundant type II collagen could be detected by immunohistochemistry in the condylar cartilage defects. Confocal microscopy revealed that implanted GFP‑labelled BMSCs were embedded in cartilage‑like tissues. The co‑culture system described herein provides a chondrogenic microenvironment to induce the chondrogenic differentiation of BMSCs in vivo without any additional cellular factors.
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Copy and paste a formatted citation
Spandidos Publications style
Sun H, Huang Y, Zhang L, Li B and Wang X: Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects. Exp Ther Med 16: 2969-2977, 2018.
APA
Sun, H., Huang, Y., Zhang, L., Li, B., & Wang, X. (2018). Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects. Experimental and Therapeutic Medicine, 16, 2969-2977. https://doi.org/10.3892/etm.2018.6551
MLA
Sun, H., Huang, Y., Zhang, L., Li, B., Wang, X."Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects". Experimental and Therapeutic Medicine 16.4 (2018): 2969-2977.
Chicago
Sun, H., Huang, Y., Zhang, L., Li, B., Wang, X."Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2969-2977. https://doi.org/10.3892/etm.2018.6551
Copy and paste a formatted citation
x
Spandidos Publications style
Sun H, Huang Y, Zhang L, Li B and Wang X: Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects. Exp Ther Med 16: 2969-2977, 2018.
APA
Sun, H., Huang, Y., Zhang, L., Li, B., & Wang, X. (2018). Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects. Experimental and Therapeutic Medicine, 16, 2969-2977. https://doi.org/10.3892/etm.2018.6551
MLA
Sun, H., Huang, Y., Zhang, L., Li, B., Wang, X."Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects". Experimental and Therapeutic Medicine 16.4 (2018): 2969-2977.
Chicago
Sun, H., Huang, Y., Zhang, L., Li, B., Wang, X."Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects". Experimental and Therapeutic Medicine 16, no. 4 (2018): 2969-2977. https://doi.org/10.3892/etm.2018.6551
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