Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects

Sun,Hao; Huang,Yue; Zhang,Lei; Li,Biao; Wang,Xudong

doi:10.3892/etm.2018.6551

Co‑culture of bone marrow stromal cells and chondrocytes in vivo for the repair of the goat condylar cartilage defects

Authors:
- Hao Sun
- Yue Huang
- Lei Zhang
- Biao Li
- Xudong Wang
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Affiliations: Department of Oral and Cranio‑Maxillofacial Surgery, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, P.R. China, Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China
Published online on: August 1, 2018 https://doi.org/10.3892/etm.2018.6551
Pages: 2969-2977
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study explored the feasibility of inducing the differentiation of BMSCs into chondrocytes through co‑culture with chondrocytes in hydrogel constructs (Pluronic F‑127 gel) in vivo for the repair of goat mandibular condylar cartilage defects. Chondrocytes and BMSCs were isolated from goat auricular cartilage and bone marrow, respectively, and were mixed at a ratio of 3:7. BMSCs were labelled with green fluorescence protein (GFP) using a retrovirus vector for tracing. Mixed cells were re‑suspended in 30% Pluronic F‑127 at a concentration of 5x107 cells/ml to form a gel‑cell complex. The gel‑cell complex was implanted into the temporomandibular joint condylar articular cartilage defects. The whole temporomandibular joint and adjacent tissues were harvested at 4, 8, and 12 weeks after surgery, and gross observation, histology and collagen II expression were evaluated. In the co‑culture group, cartilage‑like tissues were formed, and abundant type II collagen could be detected by immunohistochemistry in the condylar cartilage defects. Confocal microscopy revealed that implanted GFP‑labelled BMSCs were embedded in cartilage‑like tissues. The co‑culture system described herein provides a chondrogenic microenvironment to induce the chondrogenic differentiation of BMSCs in vivo without any additional cellular factors.

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1	Kim HK, Moran ME and Salter RB: The potential for regeneration of articular cartilage in defects created by chondral shaving and subchondral abrasion. An experimental investigation in rabbits. J Bone Joint Surg Am. 73:1301–1315. 1991. View Article : Google Scholar : PubMed/NCBI
2	Convery FR, Akeson WH and Keown GH: The repair of large osteochondral defects. An experimental study in horses. Clin Orthop Relat Res. 82:253–262. 1972. View Article : Google Scholar : PubMed/NCBI
3	Valentini V, Vetrano S, Agrillo A, Torroni A, Fabiani F and Iannetti G: Surgical treatment of TMJ ankylosis: Our experience (60 cases). J Craniofac Surg. 13:59–67. 2002. View Article : Google Scholar : PubMed/NCBI
4	Hikiji H, Takato T, Matsumoto S and Mori Y: Experimental study of reconstruction of the temporomandibular joint using a bone transport technique. J Oral Maxillofac Surg. 58:1270–1277. 2000. View Article : Google Scholar : PubMed/NCBI
5	Görtz S and Bugbee WD: Allografts in articular cartilage repair. J Bone Joint Surg Am. 88:1374–1384. 2006. View Article : Google Scholar : PubMed/NCBI
6	Hangody L, Feczkò P, Bartha L, Bodò G and Kish G: Mosaicplasty for the treatment of articular defects of the knee and ankle. Clin Orthop Relat Res. 391 Suppl:S328–S36. 2001. View Article : Google Scholar : PubMed/NCBI
7	Jamali AA, Emmerson BC, Chung C, Convery FR and Bugbee WD: Fresh osteochondral allografts: Results in the patellofemoral joint. Clin Orthop Relat Res. 176–185. 2005. View Article : Google Scholar : PubMed/NCBI
8	Liechty KW, MacKenzie TC, Shaaban AF, Radu A, Moseley AM, Deans R, Marshak DR and Flake AW: Human mesenchymal stem cells engraft and demonstrate site-specific differentiation after in utero transplantation in sheep. Nat Med. 6:1282–1286. 2000. View Article : Google Scholar : PubMed/NCBI
9	Minguell JJ, Erices A and Conget P: Mesenchymal stem cells. Exp Biol Med (Maywood). 226:507–520. 2001. View Article : Google Scholar : PubMed/NCBI
10	Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI
11	Baksh D, Yao R and Tuan RS: Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells. 25:1384–1392. 2007. View Article : Google Scholar : PubMed/NCBI
12	Li WJ, Tuli R, Huang X, Laquerriere P and Tuan RS: Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials. 26:5158–5166. 2005. View Article : Google Scholar : PubMed/NCBI
13	Yang HN, Park JS, Na K, Woo DG, Kwon YD and Park KH: The use of green fluorescence gene (GFP)-modified rabbit mesenchymal stem cells (rMSCs) co-cultured with chondrocytes in hydrogel constructs to reveal the chondrogenesis of MSCs. Biomaterials. 30:6374–6385. 2009. View Article : Google Scholar : PubMed/NCBI
14	Liu X, Sun H, Yan D, Zhang L, Lv X, Liu T, Zhang W, Liu W, Cao Y and Zhou G: In vivo ectopic chondrogenesis of BMSCs directed by mature chondrocytes. Biomaterials. 31:9406–9414. 2010. View Article : Google Scholar : PubMed/NCBI
15	Bohorquez M, Koch C, Trygstad T and Pandit N: A study of the temperature-dependent micellization of pluronic F127. J Colloid Interface Sci. 216:34–40. 1999. View Article : Google Scholar : PubMed/NCBI
16	Padilla M, Clark GT and Merrill RL: Topical medications for orofacial neuropathic pain: A review. J Am Dent Assoc. 131:184–195. 2000. View Article : Google Scholar : PubMed/NCBI
17	Ved PM and Kim K: Poly(ethylene oxide/propylene oxide) copolymer thermo-reversible gelling system for the enhancement of intranasal zidovudine delivery to the brain. Int J Pharm. 411:1–9. 2011. View Article : Google Scholar : PubMed/NCBI
18	Barichello JM, Morishita M, Takayama K, Chiba Y, Tokiwa S and Nagai T: Enhanced rectal absorption of insulin-loaded Pluronic F-127 gels containing unsaturated fatty acids. Int J Pharm. 183:125–132. 1999. View Article : Google Scholar : PubMed/NCBI
19	Desai SD and Blanchard J: Evaluation of pluronic F127-based sustained-release ocular delivery systems for pilocarpine using the albino rabbit eye model. J Pharm Sci. 87:1190–1195. 1998. View Article : Google Scholar : PubMed/NCBI
20	Wakitani S, Okabe T, Horibe S, Mitsuoka T, Saito M, Koyama T, Nawata M, Tensho K, Kato H, Uematsu K, et al: Safety of autologous bone marrow-derived mesenchymal stem cell transplantation for cartilage repair in 41 patients with 45 joints followed for up to 11 years and 5 months. J Tissue Eng Regen Med. 5:146–150. 2011. View Article : Google Scholar : PubMed/NCBI
21	Hodge WA, Fijan RS, Carlson KL, Burgess RG, Harris WH and Mann RW: Contact pressures in the human hip joint measured in vivo. Proc Natl Acad Sci USA. 83:2879–2883. 1986. View Article : Google Scholar : PubMed/NCBI
22	Madry H, Grün UW and Knutsen G: Cartilage repair and joint preservation: Medical and surgical treatment options. Dtsch Arztebl Int. 108:669–677. 2011.PubMed/NCBI
23	Becerra J, Andrades JA, Guerado E, Zamora-Navas P, Lòpez-Puertas JM and Reddi AH: Articular cartilage: Structure and regeneration. Tissue Eng Part B Rev. 16:617–627. 2010. View Article : Google Scholar : PubMed/NCBI
24	Redman SN, Oldfield SF and Archer CW: Current strategies for articular cartilage repair. Eur Cell Mater. 9:23–32. 2005. View Article : Google Scholar : PubMed/NCBI
25	Kim HK, Moran ME and Salter RB: The potential for regeneration of articular cartilage in defects created by chondral shaving and subchondral abrasion. An experimental investigation in rabbits. J Bone Joint Surg Am. 73:1301–1315. 1991. View Article : Google Scholar : PubMed/NCBI
26	Kelly DJ and Prendergast PJ: Mechano-regulation of stem cell differentiation and tissue regeneration in osteochondral defects. J Biomech. 38:1413–1422. 2005. View Article : Google Scholar : PubMed/NCBI
27	Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O and Peterson L: Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med. 331:889–895. 1994. View Article : Google Scholar : PubMed/NCBI
28	Brittberg M: Cell carriers as the next generation of cell therapy for cartilage repair: A review of the matrix-induced autologous chondrocyte implantation procedure. Am J Sports Med. 38:1259–1271. 2010. View Article : Google Scholar : PubMed/NCBI
29	Fischer J, Dickhut A, Rickert M and Richter W: Human articular chondrocytes secrete parathyroid hormone-related protein and inhibit hypertrophy of mesenchymal stem cells in coculture during chondrogenesis. Arthritis Rheum. 62:2696–2706. 2010. View Article : Google Scholar : PubMed/NCBI
30	Weiss S, Hennig T, Bock R, Steck E and Richter W: Impact of growth factors and PTHrP on early and late chondrogenic differentiation of human mesenchymal stem cells. J Cell Physiol. 223:84–93. 2010.PubMed/NCBI
31	Mueller MB and Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells. Arthritis Rheum. 58:1377–1388. 2008. View Article : Google Scholar : PubMed/NCBI
32	Williams R, Khan IM, Richardson K, Nelson L, McCarthy HE, Analbelsi T, Singhrao SK, Dowthwaite GP, Jones RE, Baird DM, et al: Identification and clonal characterisation of a progenitor cell sub-population in normal human articular cartilage. PLoS One. 5:e132462010. View Article : Google Scholar : PubMed/NCBI
33	Caplan AI: Why are MSCs therapeutic? New data: New insight. J Pathol. 217:318–324. 2009. View Article : Google Scholar : PubMed/NCBI
34	Johnstone B, Hering TM, Caplan AI, Goldberg VM and Yoo JU: In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res. 238:265–272. 1998. View Article : Google Scholar : PubMed/NCBI
35	Pelttari K, Steck E and Richter W: The use of mesenchymal stem cells for chondrogenesis. Injury. 39 Suppl 1:S58–S65. 2008. View Article : Google Scholar : PubMed/NCBI
36	Banfi A, Muraglia A, Dozin B, Mastrogiacomo M, Cancedda R and Quarto R: Proliferation kinetics and differentiation potential of ex vivo expanded human bone marrow stromal cells: Implications for their use in cell therapy. Exp Hematol. 28:707–715. 2000. View Article : Google Scholar : PubMed/NCBI
37	Zhou GD, Miao CL, Wang XY, Liu TY, Cui L, Liu W and Cao YL: Experimental study of in vitro chondrogenesis by co-culture of bone marrow stromal cells and chondrocytes. Zhonghua Yi Xue Za Zhi. 84:1716–1720. 2004.(In Chinese). PubMed/NCBI
38	Gardner OF, Archer CW, Alini M and Stoddart MJ: Chondrogenesis of mesenchymal stem cells for cartilage tissue engineering. Histol Histopathol. 28:23–42. 2013.PubMed/NCBI
39	Park SS, Jin HR, Chi DH and Taylor RS: Characteristics of tissue-engineered cartilage from human auricular chondrocytes. Biomaterials. 25:2363–2369. 2004. View Article : Google Scholar : PubMed/NCBI
40	Smeriglio P, Lai JH, Yang F and Bhutani N: 3D Hydrogel Scaffolds for articular chondrocyte culture and cartilage generation. J Vis Exp. Oct 7–2015.doi: 10.3791/53085. View Article : Google Scholar : PubMed/NCBI
41	Raghunath J, Rollo J, Sales KM, Butler PE and Seifalian AM: Biomaterials and scaffold design: Key to tissue-engineering cartilage. Biotechnol Appl Biochem. 46:73–84. 2007. View Article : Google Scholar : PubMed/NCBI
42	Kisiday J, Jin M, Kurz B, Hung H, Semino C, Zhang S and Grodzinsky AJ: Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: Implications for cartilage tissue repair. Proc Natl Acad Sci USA. 99:9996–10001. 2002. View Article : Google Scholar : PubMed/NCBI
43	Fisher JP, Jo S, Mikos AG and Reddi AH: Thermoreversible hydrogel scaffolds for articular cartilage engineering. J Biomed Mater Res A. 71:268–274. 2004. View Article : Google Scholar : PubMed/NCBI
44	Yasuda A, Kojima K, Tinsley KW, Yoshioka H, Mori Y and Vacanti CA: In vitro culture of chondrocytes in a novel thermoreversible gelation polymer scaffold containing growth factors. Tissue Eng. 12:1237–1245. 2006. View Article : Google Scholar : PubMed/NCBI
45	Kaneko Y, Nakamura S, Sakai K, Kikuchi A, Aoyagi T, Sakurai Y and Okano T: Synthesis and swelling-deswelling kinetics of poly(N-isopropylacrylamide) hydrogels grafted with LCST modulated polymers. J Biomater Sci Polym Ed. 10:1079–1091. 1999. View Article : Google Scholar : PubMed/NCBI