Open Access

Effect of autophagy‑associated proteins on the arecoline‑induced liver injury in mice

  • Authors:
    • Xia Wang
    • Xinhong Song
    • Youjiao Si
    • Jikai Xia
    • Bin Wang
    • Peiyuan Wang
  • View Affiliations

  • Published online on: August 2, 2018     https://doi.org/10.3892/etm.2018.6564
  • Pages: 3041-3049
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Arecoline can be used to treat diseases including glaucoma and tapeworm infection, however, long‑term administration can cause severe adverse effects, including oral submucosal fibrosis, oral cancer, hepatic injury and liver cancer. Autophagy serves a role in these injuries. The present study established a mouse model of arecoline‑induced hepatic injury and investigated the role of autophagy‑associated proteins in this injury. The results indicated that the expression levels of the autophagy marker protein microtubule associated protein 1 light chain 3 B (MAP1LC3B) and autophagy‑promoting protein beclin 1 were elevated in the injured hepatic cells, while the expression levels of a well‑known negative regulator of autophagy, mammalian target of rapamycin (mTOR), were reduced. Following treatment of the hepatic injury with glutathione, the liver function improved and liver damage was reduced effectively. Compared with the control group, the expression levels of both MAP1LC3B and beclin 1 were significantly upregulated in the glutathione‑treated mice, but the expression of mTOR was significantly downregulated. It may be concluded that in the process of protecting against arecoline‑induced hepatic injury, glutathione cooperates with mTOR and beclin 1 to accelerate autophagy, maintaining stable cell morphology and cellular functions.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Wang X, Song X, Si Y, Xia J, Wang B and Wang P: Effect of autophagy‑associated proteins on the arecoline‑induced liver injury in mice. Exp Ther Med 16: 3041-3049, 2018
APA
Wang, X., Song, X., Si, Y., Xia, J., Wang, B., & Wang, P. (2018). Effect of autophagy‑associated proteins on the arecoline‑induced liver injury in mice. Experimental and Therapeutic Medicine, 16, 3041-3049. https://doi.org/10.3892/etm.2018.6564
MLA
Wang, X., Song, X., Si, Y., Xia, J., Wang, B., Wang, P."Effect of autophagy‑associated proteins on the arecoline‑induced liver injury in mice". Experimental and Therapeutic Medicine 16.4 (2018): 3041-3049.
Chicago
Wang, X., Song, X., Si, Y., Xia, J., Wang, B., Wang, P."Effect of autophagy‑associated proteins on the arecoline‑induced liver injury in mice". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3041-3049. https://doi.org/10.3892/etm.2018.6564