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Article

Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells

  • Authors:
    • Jian Wang
    • Yingjie Jin
    • Jianchun Li
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China, Department of Anesthesiology, Shengze Hospital of Jiangsu, Suzhou, Jiangsu 215228, P.R. China
  • Pages: 3740-3744
    |
    Published online on: August 9, 2018
       https://doi.org/10.3892/etm.2018.6590
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Abstract

Neurosurgery always results in neuroinflammation, which may activate microglial cells. Previous studies have demonstrated that fentanyl could be used for the induction or maintenance of anesthesia prior to surgery. However, it is unknown if fentanyl attenuates neuroinflammation prophylactically. Cell viability in groups that were treated with different concentrations of fentanyl (0.01, 0.1, 1 or 5 µmol/l) was analyzed by an MTT assay. BV‑2 microglial cells were treated with lipopolysaccharide (LPS) at a concentration of 1 µg/ml to mimic neuroinflammation in vitro. BV‑2 cells were pretreated with 5 µmol/l fentanyl prior to stimulation by LPS. The protein levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑10 in the culture medium were assessed by ELISA. The mRNA level of toll‑like receptor (TLR)4 was evaluated by reverse transcription‑quantitative polymerase chain reaction analysis. The protein levels of TLR4, glycogen synthase kinase (GSK)‑3β and phosphorylated (p)‑GSK‑3β in BV‑2 cells were assessed by western blot analysis. The MTT assay demonstrated that low concentrations of fentanyl (0.01, 0.1 or 1 µmol/l) did not affect the cell viability of BV‑2 cells, while 5 µmol/l fentanyl significantly reduced BV‑2 cell viability. The results of ELISA revealed that LPS significantly upregulated the release of TNF‑α, IL‑1β and IL‑10, which were repressed by fentanyl pretreatment. Fentanyl pretreatment significantly reduced the LPS‑induced elevation of TLR4 at mRNA and protein levels as well as p‑GSK‑3β protein levels in BV‑2 cells. In conclusion, fentanyl pretreatment protects BV‑2 cells from LPS‑induced neuroinflammation by inhibiting TLR4 expression and GSK‑3β activation. Neuroinflammation induced by surgery serves an important role in the development of postoperative cognitive dysfunction (POCD) and targeting the TLR4 and GSK‑3β signaling pathway may provide a novel therapeutic approach for the treatment of POCD.
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1 

Allan SM and Rothwell NJ: Inflammation in central nervous system injury. Phil Trans R Soc Lond B Biol Sci. 358:1669–1677. 2003. View Article : Google Scholar

2 

Yan A, Zhang Y, Lin J, Song L, Wang X and Liu Z: Partial depletion of peripheral M1 macrophages reverses motor deficits in MPTP-treated mouse by suppressing neuroinflammation and dopaminergic neurodegeneration. Front Aging Neurosci. 10:1602018. View Article : Google Scholar : PubMed/NCBI

3 

Baltuch GS: Microglia as mediators of inflammatory and degenerative diseases. Annu Rev Neurosci. 22:219–240. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Lucas SM, Rothwell NJ and Gibson RM: The role of inflammation in CNS injury and disease. Br J Pharmacol. 147 Suppl 1:S232–S240. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Theoharides TC, Asadi S and Patel AB: Focal brain inflammation and autism. Neuroinflamm. 10:462013. View Article : Google Scholar

6 

Auriel E, Regev K and Korczyn AD: Nonsteroidal anti-inflammatory drugs exposure and the central nervous system. Handb Clin Neurol. 119:577–584. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Bacchi S, Palumbo P, Sponta A and Coppolino MF: Clinical pharmacology of non-steroidal anti-inflammatory drugs: A review. Antiinflamm Antiallergy Agents Med Chem. 11:52–64. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Qin L, Wu X, Block ML, Liu Y, Breese GR, Hong JS, Knapp DJ and Crews FT: Systemic LPS causes chronic neuroinflammation and progressive neurodegeneration. Glia. 55:453–462. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Wilms H, Sievers J, Rickert U, Rostami-Yazdi M, Mrowietz U and Lucius R: Dimethylfumarate inhibits microglial and astrocytic inflammation by suppressing the synthesis of nitric oxide, IL-1beta, TNF-alpha and IL-6 in an in-vitro model of brain inflammation. J Neuroinflamm. 7:302010. View Article : Google Scholar

10 

Choi Y, Lee MK, Lim SY, Sung SH and Kim YC: Inhibition of inducible NO synthase, cyclooxygenase-2 and interleukin-1beta by torilin is mediated by mitogen-activated protein kinases in microglial BV2 cells. British J Phamrmacol. 156:933–940. 2009. View Article : Google Scholar

11 

Blasi E, Barluzzi R, Bocchini V, Mazzolla R and Bistoni F: Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J Neuroimmunol. 27:229–237. 1990. View Article : Google Scholar : PubMed/NCBI

12 

Ooi YY, Ramasamy R, Rahmat Z, Subramaiam H, Tan SW, Abdullah M, Israf DA and Vidyadaran S: Bone marrow derived mesenchymal stem cells modulate BV2 microglia responses to lipopolysaccharide. Int Immunopharmacol. 10:1532–1540. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Nelson L and Schwaner R: Transdermal fentanyl: Pharmacology and toxicology. J Med Toxicol. 5:230–241. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Johnston KD: The potential for mu-opioid receptor agonists to be anti-emetic in humans: A review of clinical data. Acta Anaesthesiol Scand. 54:132–140. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Dahan A, Sarton E, Teppema L and Olievier C: Sex-related differences in the influence of morphine on ventilatory control in humans. Anesthesiology. 88:903–913. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Sarton E, Teppema L and Dahan A: Sex differences in morphine-induced ventilatory depression reside within the peripheral chemoreflex loop. Anesthesiology. 90:1329–1338. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Stein C, Clark JD, Oh U, Vasko MR, Wilcox GL, Overland AC, Vanderah TW and Spencer RH: Peripheral mechanisms of pain and analgesia. Brain Res Rev. 60:90–113. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Watkins LR, Maier SF and Goehler LE: Immune activation: The role of pro-inflammatory cytokines in inflammation, illness responses and pathological pain states. Pain. 63:289–302. 1995. View Article : Google Scholar : PubMed/NCBI

19 

McNally L, Bhagwagar Z and Hannestad J: Inflammation, glutamate, and glia in depression: A literature review. CNS Spectr. 13:501–510. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Tan SW, Ramasamy R, Abdullah M and Vidyadaran S: Inhibitory effects of palm α-, γ- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia. Cell Immunol. 271:205–209. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Łabuzek K, Liber S, Gabryel B and Okopień B: AICAR (5-aminoimidazole-4-carboxamide-1-beta-4-ribofuranoside) increases the production of toxic molecules and affects the profile of cytokines release in LPS-stimulated rat primary microglial cultures. Neurotoxicology. 31:134–146. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Gui B, Su M, Chen J, Jin L, Wan R and Qian Y: Neuroprotective effects of pretreatment with propofol in LPS induced BV-2 microglia cells: Role of TLR4 and GSK-3β. Inflammation. 35:1632–1640. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Crozier TA, Müller JE, Quittkatt D, Weyland W, Sydow M, Wuttke W and Kettler D: Interleukin-1 beta and interleukin-6-plasma concentrations in laparotomies. Interaction with neuroendocrine secretion and postoperative temperature regulation? Anaesthetist. 42:343–349. 1993.(In German).

25 

Grivennikov SI, Tumanov AV, Liepinsh DJ, Kruglov AA, Marakusha BI, Shakhov AN, Murakami T, Drutskaya LN, Förster I, Clausen BE, et al: Distinct and nonredundant in vivo functions of TNF produced by t cells and macrophages/neutrophils: Protective and deleterious effects. Immunity. 22:93–104. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Marie C, Pitton C, Fitting C and Cavaillon JM: IL-10 and IL-4 synergize with TNF-alpha to induce IL-1ra production by human neutrophils. Cytokine. 8:147–151. 1996. View Article : Google Scholar : PubMed/NCBI

27 

Owens T, Babcock AA, Millward JM and Toft-Hansen H: Cytokine and chemokine inter-regulation in the inflamed or injured CNS. Brain Res Brain Res Rev. 48:178–184. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Morzaev D, Nicholson JD, Caspi T, Weiss S, Hochhauser E and Goldenberg-Cohen N: Toll-like receptor-4 knockout mice are more resistant to optic nerve crush damage than wild-type mice. Clin Exp Ophthalmol. 43:655–665. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Lehnardt S, Lachance C, Patrizi S, Lefebvre S, Follett PL, Jensen FE, Rosenberg PA, Volpe JJ and Vartanian T: The toll-like receptor TLR4 is necessary for lipopolysaccharide-induced oligodendrocyte injury in the CNS. J Neurosci. 22:2478–2486. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Hwang D: Modulation of the expression of cyclooxygenase-2 by fatty acids mediated through toll-like receptor 4-derived signaling pathways. FASEB J. 15:2556–2564. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Willis LM, Bielinski DF, Fisher DR, Matthan NR and Joseph JA: Walnut extract inhibits LPS-induced activation of BV-2 microglia via internalization of TLR4: Possible involvement of phospholipase D2. Inflamm. 33:325–333. 2010. View Article : Google Scholar

32 

Barsness KA, Arcaroli J, Harken AH, Abraham E, Banerjee A, Reznikov L and McIntyre RC: Hemorrhage-induced acute lung injury is TLR-4 dependent. Am J Physiol Regul Integr Comp Physiol. 287:R592–R599. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Hu X, Paik PK, Chen J, Yarilina A, Kockeritz L, Lu TT, Woodgett JR and Ivashkiv LB: IFN-gamma suppresses IL-10 production and synergizes with TLR2 by regulating GSK3 and CREB/AP-1 proteins. Immunity. 24:563–574. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Rodionova E, Conzelmann M, Maraskovsky E, Hess M, Kirsch M, Giese T, Ho AD, Zöller M, Dreger P and Luft T: GSK-3 mediates differentiation and activation of proinflammatory dendritic cells. Blood. 109:1584–1592. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Jin Y and Li J: Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells. Exp Ther Med 16: 3740-3744, 2018.
APA
Wang, J., Jin, Y., & Li, J. (2018). Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells. Experimental and Therapeutic Medicine, 16, 3740-3744. https://doi.org/10.3892/etm.2018.6590
MLA
Wang, J., Jin, Y., Li, J."Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells". Experimental and Therapeutic Medicine 16.4 (2018): 3740-3744.
Chicago
Wang, J., Jin, Y., Li, J."Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3740-3744. https://doi.org/10.3892/etm.2018.6590
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Jin Y and Li J: Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells. Exp Ther Med 16: 3740-3744, 2018.
APA
Wang, J., Jin, Y., & Li, J. (2018). Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells. Experimental and Therapeutic Medicine, 16, 3740-3744. https://doi.org/10.3892/etm.2018.6590
MLA
Wang, J., Jin, Y., Li, J."Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells". Experimental and Therapeutic Medicine 16.4 (2018): 3740-3744.
Chicago
Wang, J., Jin, Y., Li, J."Protective role of fentanyl in lipopolysaccharide‑induced neuroinflammation in BV‑2 cells". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3740-3744. https://doi.org/10.3892/etm.2018.6590
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