Protective role of propofol in endometriosis and its mechanism

Feng,Shuo; Sun,Yingui

doi:10.3892/etm.2018.6648

Protective role of propofol in endometriosis and its mechanism

Authors:
- Shuo Feng
- Yingui Sun
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Affiliations: Department of Gynecology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261053, P.R. China, Department of Anesthesiology, Weifang Medical University, Weifang, Shandong 261053, P.R. China
Published online on: August 22, 2018 https://doi.org/10.3892/etm.2018.6648
Pages: 3646-3650

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Abstract

Endometriosis (EM) is a common benign gynecological disorder. The present study aimed to investigate the potential role of propofol, a commonly used intravenous anesthetic agent, in the pathogenesis of EM. The EM cell line CRL‑7566 was used in the present study. CRL‑7566 cells were first treated with various concentrations of propofol (0, 1, 5 or 10 µg/ml) for specific duration, and the cell viability and apoptotic rate were determined by performing an MTT and a flow cytometric cell apoptosis assay, respectively. The protein and mRNA levels of cell proliferation‑ and apoptosis‑associated genes were detected by western blot and reverse‑transcription quantitative polymerase chain reaction, respectively. The results demonstrated that propofol inhibited CRL‑7566 cell proliferation in a dose‑ and time‑dependent manner. CRL‑7566 cell apoptosis was dose‑dependently induced by propofol treatment. In addition, propofol treatment significantly increased the levels of forkhead box (FOX)O1, FOXO3, Bim, pro‑caspase‑3, active caspase‑3, p53 and p21. In conclusion, the present study suggested that propofol inhibited the proliferation and induced apoptosis of EM cells via inducing the expression/activation of multiple associated genes/proteins, indicating a protective role of propofol in EM.

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