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Article

Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus

  • Authors:
    • Hui Wang
    • Zhengxia Wang
    • Qingbin Tang
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China, Clinical Skills Center, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China, Emergency Medicine Department, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China
  • Pages: 3639-3645
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    Published online on: August 24, 2018
       https://doi.org/10.3892/etm.2018.6655
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Abstract

The aim of the present study was to investigate the function and mechanism of action of microRNA (miRNA or miR)‑199a‑3p in vascular endothelial cell injury induced by type 2 diabetes mellitus (T2DM). A total of 36 patients with T2DM (26 males and 10 females; mean age, 52.5±7.0 years) and 20 healthy subjects (10 males and 10 females; mean age, 55.6±4.5 years) were included in the present study. Peripheral blood samples were obtained from all participants and total RNA was extracted Reverse transcription‑quantitative polymerase chain reaction was performed to determine the expression of miR‑199a‑3p. Following the transfection of human umbilical vein endothelial cells (HUVECs) with a negative control (NC) miRNA or miR‑199a‑3p mimics, cell proliferation was assessed using a Cell Counting kit‑8 assay. Cell migration was investigated using Transwell assays and flow cytometry was performed to detect the apoptosis of HUVECs. HUVECs were infected with Ad‑GFP‑LC3B and laser‑scanning confocal microscopy was performed to observe autophagosomes in HUVECs. Western blotting was used to measure the expression of proteins associated with autophagy and the phosphatidylinositol 3‑kinase (PI3K)/protein kinase B (AKT)/nuclear factor (NF)‑κB signaling pathway. MiR‑199a‑3p was downregulated in peripheral blood from patients with T2DM compared with healthy subjects. Transfection with miR‑199a‑3p mimics promoted the proliferation and migration of HUVECs. However, miR‑199a‑3p overexpression inhibited the apoptosis of HUVECs. MiR‑199a‑3p facilitated HUVEC autophagy by affecting autophagy‑associated signaling pathways. Furthermore, miR‑199a‑3p regulated the biological functions of HUVECs via the PI3K/AKT/NF‑κB signaling pathway. The results of the present study suggest that miR‑199a‑3p expression was reduced in patients with T2DM compared with healthy subjects and may be associated with vascular endothelial cell injury. In addition, miR‑199a‑3p promoted the proliferation, migration and autophagy of HUVECs, potentially by regulating the PI3K/AKT/NF‑κB signaling pathway. Therefore, miR‑199a‑3p may function as protector of vascular endothelia.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Wang Z and Tang Q: Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus. Exp Ther Med 16: 3639-3645, 2018.
APA
Wang, H., Wang, Z., & Tang, Q. (2018). Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus. Experimental and Therapeutic Medicine, 16, 3639-3645. https://doi.org/10.3892/etm.2018.6655
MLA
Wang, H., Wang, Z., Tang, Q."Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus". Experimental and Therapeutic Medicine 16.4 (2018): 3639-3645.
Chicago
Wang, H., Wang, Z., Tang, Q."Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3639-3645. https://doi.org/10.3892/etm.2018.6655
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Wang Z and Tang Q: Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus. Exp Ther Med 16: 3639-3645, 2018.
APA
Wang, H., Wang, Z., & Tang, Q. (2018). Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus. Experimental and Therapeutic Medicine, 16, 3639-3645. https://doi.org/10.3892/etm.2018.6655
MLA
Wang, H., Wang, Z., Tang, Q."Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus". Experimental and Therapeutic Medicine 16.4 (2018): 3639-3645.
Chicago
Wang, H., Wang, Z., Tang, Q."Reduced expression of microRNA‑199a‑3p is associated with vascular endothelial cell injury induced by type 2 diabetes mellitus". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3639-3645. https://doi.org/10.3892/etm.2018.6655
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