MicroRNA‑34a suppresses breast cancer cell proliferation and invasion by targeting Notch1

Rui,Xiaoping; Zhao,Haibin; Xiao,Xianqiu; Wang,Li; Mo,Lin; Yao,Yao

doi:10.3892/etm.2018.6744

MicroRNA‑34a suppresses breast cancer cell proliferation and invasion by targeting Notch1

Authors:
- Xiaoping Rui
- Haibin Zhao
- Xianqiu Xiao
- Li Wang
- Lin Mo
- Yao Yao
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Affiliations: Department of Thyroid Breast Surgery, Wuxi Taihu Hospital, Wuxi, Jiangsu 214044, P.R. China, Department of Pathology, Wuxi Taihu Hospital, Wuxi, Jiangsu 214044, P.R. China
Published online on: September 17, 2018 https://doi.org/10.3892/etm.2018.6744
Pages: 4387-4392
Copyright: © Rui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRs) have been reported to serve critical roles in the progression of tumors. However, thus far, the role of miR‑34a in breast cancer is largely unknown. Therefore, the present study aimed to investigate the expression and role of miR‑34a in breast cancer, and to further explore the underlying molecular mechanism. Reverse transcription‑quantitative polymerase chain reaction was performed to detect the level of miR‑34a in human breast cancer tissues. In addition, the role of miR‑34a in MCF‑7 breast cancer cells was investigated by performing miR‑34a overexpression or downregulation through transfection with miR‑34a mimic or inhibitor, respectively. Next, the viability and invasion of the MCF‑7 cells were respectively analyzed by MTT assay and transwell assay, while apoptosis and cell cycle progression were examined by flow cytometry. Furthermore, associated protein levels were measured using western blotting. The results demonstrated that miR‑34a was downregulated in human breast cancer tissues in comparison with the adjacent normal tissues. In addition, Notch1 was demonstrated to be a direct target of miR‑34a. miR‑34a mimic transfection inhibited MCF‑7 cell viability, induced cell apoptosis and G1 phase arrest, and prevented cell invasion, while miR‑34a inhibitor transfection resulted in the opposite effects. In conclusion, the presented data indicated that miR‑34a suppressed breast cancer cell proliferation and invasion, and its effect may partly be exerted by targeting Notch1.

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