Expression of microRNA-23b in patients with sepsis and its effect on leukocytes and the expression of E-selectin and ICAM-1

Ou,Hao; Xiao,Xianzhong; Jiang,Yu; Peng,Yue; Yang,Mingshi; Gao,Min

doi:10.3892/etm.2018.6759

Expression of microRNA-23b in patients with sepsis and its effect on leukocytes and the expression of E-selectin and ICAM-1

Authors:
- Hao Ou
- Xianzhong Xiao
- Yu Jiang
- Yue Peng
- Mingshi Yang
- Min Gao
View Affiliations

Affiliations: Translational Medicine Center of Sepsis, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Hunan Provincial Institute of Emergency Medicine, Hunan Provincial People's Hospital, Changsha, Hunan 410005, P.R. China
Published online on: September 18, 2018 https://doi.org/10.3892/etm.2018.6759
Pages: 4707-4711
Copyright: © Ou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The expression of microRNA-23b in peripheral blood leukocytes of patients with sepsis was investigated to assess the correlations with leukocyte, E-selectin, ICAM-1 and disease severity. The expression of microRNA-23b in peripheral blood leukocytes from 87 patients with sepsis, 50 patients with systemic inflammatory response syndrome (SIRS) and 50 normal controls were measured by reverse transcription‑quantitative PCR (RT-qPCR), and stability of microRNA-23b was evaluated. Enzyme-linked immunosorbent assay (ELISA) was used to detect E-selectin and ICAM-1. Sequential organ failure assessment (SOFA) scoring system was used to assess the severity of sepsis patients. Correlation analysis was performed between microRNA-23b and the total number of white blood cells (WBC), E-selectin, ICAM-1, and SOFA scores. Compared with the normal control group, the expression level of microRNA-23b in the sepsis group was significantly decreased (P<0.05), and WBC and E-selectin were significantly increased (P<0.05). ICAM-1 level in the sepsis and the SIRS groups was significantly higher than that in the control group (P<0.05), and it is also higher in the sepsis group than in the SIRS group. In the sepsis group, the expression level of microRNA-23b in the death group was significantly lower than that in the survivor group (P<0.05), while the level of E-selectin, ICAM-1, and SOFA scores were significantly higher in the death group than in the survivor group (P<0.05), while there was no significant difference in WBC between the groups (P>0.05). The expression level of microRNA-23b in patients with sepsis was significantly negatively correlated with SOFA scores, E-selectin, and ICAM-1 (r=-0.633, -0.585, and -0.439, respectively, P<0.05). The expression of microRNA‑23b in peripheral blood of patients with sepsis is related to the manifestation of the inflammatory state, and can be used to judge the severity and prognosis of patients with this disease.

View Figures

View References

1	Caserta S, Kern F, Cohen J, Drage S, Newbury SF and Llewelyn MJ: Circulating plasma microRNAs can differentiate human sepsis and systemic inflammatory response syndrome (SIRS). Sci Rep. 6:280062016. View Article : Google Scholar : PubMed/NCBI
2	Santana H, Sousa Ítalo P, Marques LM, Figueiredo TB and Silva RAAD: Distinct strains of Staphylococcus aureus lead to different inflammatory response patterns in a murine model of intradermal infection. Acta Sci Biol Sci. 38:457–464. 2016. View Article : Google Scholar
3	Suresh PK, Minal J, Rao PS, Ballal K, Sridevi HB and Padyana M: Volume conductivity and scatter parameters as an indicator of acute bacterial infections by the automated haematology analyser. J Clin Diagn Res. 10:EC01–EC03. 2016.
4	Moitra R, Beal DR, Belikoff BG and Remick DG: Presence of preexisting antibodies mediates survival in sepsis. Shock. 37:56–62. 2012. View Article : Google Scholar : PubMed/NCBI
5	Behbahani GD, Ghahhari NM, Javidi MA, Molan AF, Feizi N and Babashah S: MicroRNA-mediated post-transcriptional regulation of epithelial to mesenchymal transition in cancer. Pathol Oncol Res. 23:1–12. 2017. View Article : Google Scholar : PubMed/NCBI
6	Fukumoto I, Koshizuka K, Hanazawa T, Kikkawa N, Matsushita R, Kurozumi A, Kato M, Okato A, Okamoto Y and Seki N: The tumor-suppressive microRNA-23b/27b cluster regulates the MET oncogene in oral squamous cell carcinoma. Int J Oncol. 49:1119–1129. 2016. View Article : Google Scholar : PubMed/NCBI
7	Goto Y, Kojima S, Nishikawa R, Enokida H, Chiyomaru T, Kinoshita T, Nakagawa M, Naya Y, Ichikawa T and Seki N: The microRNA-23b/27b/24-1 cluster is a disease progression marker and tumor suppressor in prostate cancer. Oncotarget. 5:7748–7759. 2014. View Article : Google Scholar : PubMed/NCBI
8	Chiyomaru T, Seki N, Inoguchi S, Ishihara T, Mataki H, Matsushita R, Goto Y, Nishikawa R, Tatarano S, Itesako T, et al: Dual regulation of receptor tyrosine kinase genes EGFR and c-Met by the tumor-suppressive microRNA-23b/27b cluster in bladder cancer. Int J Oncol. 46:487–496. 2015. View Article : Google Scholar : PubMed/NCBI
9	Enelund L, Nielsen LN and Cirera S: Evaluation of microRNA stability in plasma and serum from healthy dogs. Microrna. 6:42–52. 2017. View Article : Google Scholar : PubMed/NCBI
10	Nassar FJ, Nasr R and Talhouk R: MicroRNAs as biomarkers for early breast cancer diagnosis, prognosis and therapy prediction. Pharmacol Ther. 172:34–49. 2017. View Article : Google Scholar : PubMed/NCBI
11	Chvojka J and Matějovič M: International guidelines for management of severe sepsis and septic shock 2012 - comment. Vnitr Lek. 60:59–67. 2014.(In Czech). PubMed/NCBI
12	Minne L, Abu-Hanna A and de Jonge E: Evaluation of SOFA-based models for predicting mortality in the ICU: A systematic review. Crit Care. 12:R1612008. View Article : Google Scholar : PubMed/NCBI
13	Roderburg C, Luedde M, Cardenas Vargas D, Vucur M, Scholten D, Frey N, Koch A, Trautwein C, Tacke F and Luedde T: Circulating microRNA-150 serum levels predict survival in patients with critical illness and sepsis. PLoS One. 8:e546122013. View Article : Google Scholar : PubMed/NCBI
14	Hervé M and Ibrahim EC: MicroRNA screening identifies a link between NOVA1 expression and a low level of IKAP in familial dysautonomia. Dis Model Mech. 9:899–909. 2016. View Article : Google Scholar : PubMed/NCBI
15	Cheng HS, Sivachandran N, Lau A, Boudreau E, Zhao JL, Baltimore D, Delgado-Olguin P, Cybulsky MI and Fish JE: MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways. EMBO Mol Med. 5:1017–1034. 2013. View Article : Google Scholar : PubMed/NCBI
16	Zhou H, Guo JM, Lou YR, Zhang XJ, Zhong FD, Jiang Z, Cheng J and Xiao BX: Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using microRNA as a marker. J Mol Med (Berl). 88:709–717. 2010. View Article : Google Scholar : PubMed/NCBI
17	Barzago C, Lum J, Cavalcante P, Srinivasan KG, Faggiani E, Camera G, Bonanno S, Andreetta F, Antozzi C, Baggi F, et al: A novel infection- and inflammation-associated molecular signature in peripheral blood of myasthenia gravis patients. Immunobiology. 221:1227–1236. 2016. View Article : Google Scholar : PubMed/NCBI
18	Velázquez F, Grodecki-Pena A, Knapp A, Salvador AM, Nevers T, Croce K and Alcaide P: CD43 functions as an E-Selectin ligand for Th17 cells in vitro and is required for rolling on the vascular endothelium and Th17 cell recruitment during inflammation in vivo. J Immunol. 196:1305–1316. 2016. View Article : Google Scholar : PubMed/NCBI
19	Nimrichter L, Burdick MM, Aoki K, Laroy W, Fierro MA, Hudson SA, Von Seggern CE, Cotter RJ, Bochner BS, Tiemeyer M, et al: E-selectin receptors on human leukocytes. Blood. 112:3744–3752. 2008. View Article : Google Scholar : PubMed/NCBI
20	Kung CT, Hsiao SY, Su CM, Tsai TC, Cheng HH, Tsai NW, Chang WN, Huang CR, Wang HC, Lin WC, et al: Serum adhesion molecules as predictors of bacteremia in adult severe sepsis patients at the emergency department. Clin Chim Acta. 421:116–120. 2013. View Article : Google Scholar : PubMed/NCBI
21	Cahill PA and Redmond EM: Vascular endothelium - Gatekeeper of vessel health. Atherosclerosis. 248:97–109. 2016. View Article : Google Scholar : PubMed/NCBI
22	Raffray L, Giry C, Thirapathi Y, Reboux AH, Jaffar-Bandjee MC and Gasque P: Increased levels of soluble forms of E-selectin and ICAM-1 adhesion molecules during human leptospirosis. PLoS One. 12:e01804742017. View Article : Google Scholar : PubMed/NCBI
23	Figueras-Aloy J, Gómez-López L, Rodríguez-Miguélez JM, Salvia-Roiges MD, Jordán-García I, Ferrer-Codina I, Carbonell-Estrany X and Jiménez-González R: Serum soluble ICAM-1, VCAM-1, L-selectin, and P-selectin levels as markers of infection and their relation to clinical severity in neonatal sepsis. Am J Perinatol. 24:331–338. 2007. View Article : Google Scholar : PubMed/NCBI
24	Bedirli N, Demirtas CY, Akkaya T, Salman B, Alper M, Bedirli A and Pasaoglu H: Volatile anesthetic preconditioning attenuated sepsis induced lung inflammation. J Surg Res. 178:e17–e23. 2012. View Article : Google Scholar : PubMed/NCBI