Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2018 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2018 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects

  • Authors:
    • Shiqiang Ruan
    • Jiang Deng
    • Ling Yan
    • Wenliang Huang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics Surgery, The First People's Hospital of Zunyi, Zunyi, Guizhou 563003, P.R. China
    Copyright: © Ruan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4569-4577
    |
    Published online on: September 19, 2018
       https://doi.org/10.3892/etm.2018.6776
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Articular cartilage is avascular and aneural, and has limited capacity for self‑regeneration when injured. Tissue engineering has emerged as a promising approach in repairing cartilage defects. To improve the therapy of cartilage healing, the present study investigated the efficacy of the combination of lentivirus‑mediated bone morphogenetic protein‑2 (BMP2) in bone marrow‑derived stromal cells (BMSCs) and platelet‑rich plasma (PRP) on cartilage and bone healing in a cartilage defect model using the rabbit knee. The BMSCs were harvested from New Zealand rabbits and transduced with lentivirus carrying BMP‑2. Standard bone defects were introduced in the femoral groove of patellofemoral joints of 48 New Zealand rabbits. The cartilage defects were subjected to synthetic scaffold mosaicplasty with chitosan/silk fibroin/nanohydroxyapatite particles tri‑component scaffolds soaked in BMSCs and PRP. After 16 weeks, the tissue specimens were assessed by micro‑computed tomography (micro‑CT) and macroscopic examination. The results showed that lentivirus‑mediated BMP‑2 and PRP increased the cell viability of the BMSCs, induced the expression of associated genes and enhanced osteogenic differentiation in vitro. In vivo, the expression of BMP‑2 was observed for 16 weeks. The combination of BMP‑2 and PRP treatment led to optimal results, compared with the other groups on micro‑CT and gross observations. The results of the present study present a novel therapy using the lentivirus‑mediated BMP‑2 gene together with PRP for cartilage healing.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Nehrer S, Chiari C, Domayer S, Barkay H and Yayon A: Results of chondrocyte implantation with a fibrin-hyaluronan matrix: A preliminary study. Clin Orthop Relat Res. 466:1849–1855. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Steadman JR, Rodkey WG and Rodrigo JJ: Microfracture: Surgical technique and rehabilitation to treat chondral defects. Clin Orthop Relat Re S362-S369. 2001. View Article : Google Scholar

3 

Bittberg M: Articular cartilage repair: An update on different clinical repair methods. Ortop Traumatol Rehabil. 3:235–243. 2001.PubMed/NCBI

4 

Mendicino RW, Catanzariti AR and Hallivis R: Mosaicplasty for the treatment of osteochondral defects of the ankle joint. Clin Podiatr Med Surg. 18:495–513. 2001.PubMed/NCBI

5 

Bentley G, Biant LC, Carrington RW, Akmal M, Goldberg A, Williams AM, Skinner JA and Pringle J: A prospective, randomised comparison of autologous chondrocyte implantation versus mosaicplasty for osteochondral defects in the knee. J Bone Joint Surg Br. 85:223–230. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Brittberg M, Tallheden T, Sjogren-Jansson B, Lindahl A and Peterson L: Autologous chondrocytes used for articular cartilage repair: An update. Clin Orthop Relat Res. 391 Suppl:S337–S348. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Hunziker EB: Articular cartilage repair: Basic science and clinical progress. A review of the current status and prospects. Osteoarthritis Cartilage. 10:432–463. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Hangody L, Rathonyi GK, Duska Z, Vasarhelyi G, Fules P and Módis L: Autologous osteochondral mosaicplasty. Surgical technique. J Bone Joint Surg Am. 86-A Suppl 1:S65–S72. 2004. View Article : Google Scholar

9 

Ozturk A, Ozdemir MR and Ozkan Y: Osteochondral autografting (mosaicplasty) in grade IV cartilage defects in the knee joint: 2- to 7-year results. Int Orthop. 30:200–204. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Haklar U, Tuzuner T, Kocaoglu B and Guven O: Mosaicplasty technique in the treatment of osteochondral lesions of the knee. Acta Orthop Traumatol Turc. 42:344–349. 2008.(In Turkish). View Article : Google Scholar : PubMed/NCBI

11 

Rose T, Craatz S, Hepp P, Raczynski C, Weiss J, Josten C and Lill H: The autologous osteochondral transplantation of the knee: Clinical results, radiographic findings and histological aspects. Arch Orthop Trauma Surg. 125:628–637. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Hangody L, Vasarhelyi G, Hangody LR, Sukosd Z, Tibay G, Bartha L and Bodó G: Autologous osteochondral grafting-technique and long-term results. Injury. 39 Suppl 1:S32–S39. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Emre TY, Ege T, Kose O, Demircioglu Tekdos D, Seyhan B and Uzun M: Factors affecting the outcome of osteochondral autografting (mosaicplasty) in articular cartilage defects of the knee joint: Retrospective analysis of 152 cases. Arch Orthop Trauma Surg. 133:531–536. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Hangody L, Dobos J, Balo E, Panics G, Hangody LR and Berkes I: Clinical experiences with autologous osteochondral mosaicplasty in an athletic population: A 17-year prospective multicenter study. Am J Sports Med. 38:1125–1133. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Langer R and Vacanti JP: Tissue engineering. Science. 260:920–926. 1993. View Article : Google Scholar : PubMed/NCBI

16 

Bhardwaj N and Kundu SC: Silk fibroin protein and chitosan polyelectrolyte complex porous scaffolds for tissue engineering applications. Carbohydrate Polymers. 85:325–333. 2011. View Article : Google Scholar

17 

Bhardwaj N, Nguyen QT, Chen AC, Kaplan DL, Sah RL and Kundu SC: Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering. Biomaterials. 32:5773–5781. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Ji WC, Zhang XW and Qiu YS: Selected suitable seed cell, scaffold and growth factor could maximize the repair effect using tissue engineering method in spinal cord injury. World J Exp Med. 6:58–62. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Qi XN, Mou ZL, Zhang J and Zhang ZQ: Preparation of chitosan/silk fibroin/hydroxyapatite porous scaffold and its characteristics in comparison to bi-component scaffolds. J Biomed Mater Res A. 102:366–372. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Garg P, Mazur MM, Buck AC, Wandtke ME, Liu J and Ebraheim NA: Prospective Review of Mesenchymal Stem Cells Differentiation into Osteoblasts. Orthop Surg. 9:13–19. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Fellows CR, Matta C, Zakany R, Khan IM and Mobasheri A: Adipose, bone marrow and synovial joint-derived mesenchymal stem cells for cartilage repair. Front Genet. 7:2132016. View Article : Google Scholar : PubMed/NCBI

22 

Yamasaki S, Mera H, Itokazu M, Hashimoto Y and Wakitani S: Cartilage repair with autologous bone marrow mesenchymal stem cell transplantation: Review of preclinical and clinical studies. Cartilage. 5:196–202. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Potier E, Noailly J and Ito K: Directing bone marrow-derived stromal cell function with mechanics. J Biomech. 43:807–817. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Chen Q, Shou P, Zheng C, Jiang M, Cao G, Yang Q, Cao J, Xie N, Velletri T, Zhang X, et al: Fate decision of mesenchymal stem cells: adipocytes or osteoblasts? Cell Death Differ. 23:1128–1139. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Schneider RK, Anraths J, Kramann R, Bornemann J, Bovi M, Knüchel R and Neuss S: The role of biomaterials in the direction of mesenchymal stem cell properties and extracellular matrix remodelling in dermal tissue engineering. Biomaterials. 31:7948–7959. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Abe E: Function of BMPs and BMP antagonists in adult bone. Ann N Y Acad Sci. 1068:41–53. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Lochab AK and Extavour CG: Bone morphogenetic protein (BMP) signaling in animal reproductive system development and function. Dev Biol. 427:258–269. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Zhang Q, He QF, Zhang TH, Yu XL, Liu Q and Deng FL: Improvement in the delivery system of bone morphogenetic protein-2: A new approach to promote bone formation. Biomed Mater. 7:0450022012. View Article : Google Scholar : PubMed/NCBI

29 

Starman JS, Bosse MJ, Cates CA and Norton HJ: Recombinant human bone morphogenetic protein-2 use in the off-label treatment of nonunions and acute fractures: A retrospective review. J Trauma Acute Care Surg. 72:676–681. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Angle SR, Sena K, Sumner DR, Virkus WW and Virdi AS: Healing of rat femoral segmental defect with bone morphogenetic protein-2: A dose response study. J Musculoskelet Neuronal Interact. 12:28–37. 2012.PubMed/NCBI

31 

Hsu WK, Sugiyama O, Park SH, Conduah A, Feeley BT, Liu NQ, Krenek L, Virk MS, An DS, Chen IS and Lieberman JR: Lentiviral-mediated BMP-2 gene transfer enhances healing of segmental femoral defects in rats. Bone. 40:931–938. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Miyazaki M, Sugiyama O, Tow B, Zou J, Morishita Y, Wei F, Napoli A, Sintuu C, Lieberman JR and Wang JC: The effects of lentiviral gene therapy with bone morphogenetic protein-2-producing bone marrow cells on spinal fusion in rats. J Spinal Disord Tech. 21:372–379. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Jiang X, Zhao J, Wang S, Sun X, Zhang X, Chen J, Kaplan DL and Zhang Z: Mandibular repair in rats with premineralized silk scaffolds and BMP-2-modified bMSCs. Biomaterials. 30:4522–4532. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Xu XL, Tang T, Dai K, Zhu Z, Guo XE, Yu C and Lou J: Immune response and effect of adenovirus-mediated human BMP-2 gene transfer on the repair of segmental tibial bone defects in goats. Acta Orthop. 76:637–646. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Xiao C, Zhou H, Ge S, Tang T, Hou H, Luo M and Fan X: Repair of orbital wall defects using biocoral scaffolds combined with bone marrow stem cells enhanced by human bone morphogenetic protein-2 in a canine model. Int J Mol Med. 26:517–525. 2010.PubMed/NCBI

36 

Wozney JM and Rosen V: Bone morphogenetic protein and bone morphogenetic protein gene family in bone formation and repair. Clin Orthop Relat Res. 1–37. 1998.PubMed/NCBI

37 

Chen Y, Luk KD, Cheung KM, Xu R, Lin MC, Lu WW, Leong JC and Kung HF: Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors. Gene Ther. 10:1345–1353. 2003. View Article : Google Scholar : PubMed/NCBI

38 

Park J, Ries J, Gelse K, Kloss F, von der Mark K, Wiltfang J, Neukam FW and Schneider H: Bone regeneration in critical size defects by cell-mediated BMP-2 gene transfer: A comparison of adenoviral vectors and liposomes. Gene Ther. 10:1089–1098. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Ramezani A, Hawley TS and Hawley RG: Lentiviral vectors for enhanced gene expression in human hematopoietic cells. Mol Ther. 2:458–469. 2000. View Article : Google Scholar : PubMed/NCBI

40 

Sugiyama O, An DS, Kung SP, Feeley BT, Gamradt S, Liu NQ, Chen IS and Lieberman JR: Lentivirus-mediated gene transfer induces long-term transgene expression of BMP-2 in vitro and new bone formation in vivo. Mol Ther. 11:390–398. 2005. View Article : Google Scholar : PubMed/NCBI

41 

Virk MS, Conduah A, Park SH, Liu N, Sugiyama O, Cuomo A, Kang C and Lieberman JR: Influence of short-term adenoviral vector and prolonged lentiviral vector mediated bone morphogenetic protein-2 expression on the quality of bone repair in a rat femoral defect model. Bone. 42:921–931. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Mehta S and Watson JT: Platelet rich concentrate: Basic science and current clinical applications. J Orthop Trauma. 22:432–438. 2008. View Article : Google Scholar : PubMed/NCBI

43 

Yamada Y, Ueda M, Naiki T, Takahashi M, Hata K and Nagasaka T: Autogenous injectable bone for regeneration with mesenchymal stem cells and platelet-rich plasma: Tissue-engineered bone regeneration. Tissue Eng. 10:955–964. 2004. View Article : Google Scholar : PubMed/NCBI

44 

Oliva A, Passaro I, Di Pasquale R, Di Feo A, Criscuolo M, Zappia V, Della Ragione F, D'Amato S, Annunziata M and Guida L: Ex vivo expansion of bone marrow stromal cells by platelet-rich plasma: A promising strategy in maxillo-facial surgery. Int J Immunopathol Pharmacol. 18:47–53. 2005.PubMed/NCBI

45 

Park CG, Joo MW, Jeong J, Kang YK and Lee DR: Evaluation of the effects of the combination of autologous mesenchymal stem cells and platelet-rich plasma on structural bone allograft healing. Cell Tissue Bank. 18:229–238. 2017. View Article : Google Scholar : PubMed/NCBI

46 

Du X, Huang F, Zhang S, Yao Y, Chen Y, Huang H and Bai B: Carboxymethylcellulose with phenolic hydroxyl microcapsules enclosinggene-modified BMSCs for controlled BMP-2 release in vitro. Artif Cells Nanomed Biotechnol. 45:1–14. 2017. View Article : Google Scholar

47 

Wang SJ, Jiang D, Zhang ZZ, Huang AB, Qi YS, Wang HJ, Zhang JY and Yu JK: Chondrogenic potential of peripheral blood derived mesenchymal stem cells seeded on demineralized cancellous bone scaffolds. Sci Rep. 6:364002016. View Article : Google Scholar : PubMed/NCBI

48 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

49 

Miller L: Analyzing gels and western blots with Image. J. Lukemiller. org. Miscellaneous Topics Vaguely Related to Science¸. 2010.

50 

Chiu LH, Lai WF, Chang SF, Wong CC, Fan CY, Fang CL and Tsai YH: The effect of type II collagen on MSC osteogenic differentiation and bone defect repair. Biomaterials. 35:2680–2691. 2014. View Article : Google Scholar : PubMed/NCBI

51 

Hu Q, Li B, Wang M and Shen J: Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in situ hybridization: A potential material as internal fixation of bone fracture. Biomaterials. 25:779–785. 2004. View Article : Google Scholar : PubMed/NCBI

52 

Frohbergh ME, Katsman A, Botta GP, Lazarovici P, Schauer CL, Wegst UG and Lelkes PI: Electrospun hydroxyapatite-containing chitosan nanofibers crosslinked with genipin for bone tissue engineering. Biomaterials. 33:9167–9178. 2012. View Article : Google Scholar : PubMed/NCBI

53 

Eppley BL, Woodell JE and Higgins J: Platelet quantification and growth factor analysis from platelet-rich plasma: Implications for wound healing. Plast Reconstr Surg. 114:1502–1508. 2004. View Article : Google Scholar : PubMed/NCBI

54 

Bahmanpour SP, Ghasemi MP, Sadeghi-Naini MM and Kashani IRP: Effects of platelet-rich plasma & platelet-rich fibrin with and without stromal cell-derived factor-1 on repairing full-thickness cartilage defects in knees of rabbits. Iran J Med Sci. 41:507–517. 2016.PubMed/NCBI

55 

Lian Z, Yin X, Li H, Jia L, He X, Yan Y, Liu N, Wan K, Li X and Lin S: Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma in streptozotocin-induced diabetic rats. Ann Dermatol. 26:1–10. 2014. View Article : Google Scholar : PubMed/NCBI

56 

Dong Y, Zhang Q, Li Y, Jiang J and Chen S: Enhancement of tendon-bone healing for anterior cruciate ligament (ACL) reconstruction using bone marrow-derived mesenchymal stem cells infected with BMP-2. Int J Mol Sci. 13:13605–13620. 2012. View Article : Google Scholar : PubMed/NCBI

57 

Itoi T, Harada Y, Irie H, Sakamoto M, Tamura K, Yogo T, Soeta S, Amasaki H, Hara Y and Tagawa M: Escherichia coli-derived recombinant human bone morphogenetic protein-2 combined with bone marrow-derived mesenchymal stromal cells improves bone regeneration in canine segmental ulnar defects. BMC Vet Res. 12:2012016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ruan S, Deng J, Yan L and Huang W: Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects. Exp Ther Med 16: 4569-4577, 2018.
APA
Ruan, S., Deng, J., Yan, L., & Huang, W. (2018). Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects. Experimental and Therapeutic Medicine, 16, 4569-4577. https://doi.org/10.3892/etm.2018.6776
MLA
Ruan, S., Deng, J., Yan, L., Huang, W."Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects". Experimental and Therapeutic Medicine 16.6 (2018): 4569-4577.
Chicago
Ruan, S., Deng, J., Yan, L., Huang, W."Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4569-4577. https://doi.org/10.3892/etm.2018.6776
Copy and paste a formatted citation
x
Spandidos Publications style
Ruan S, Deng J, Yan L and Huang W: Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects. Exp Ther Med 16: 4569-4577, 2018.
APA
Ruan, S., Deng, J., Yan, L., & Huang, W. (2018). Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects. Experimental and Therapeutic Medicine, 16, 4569-4577. https://doi.org/10.3892/etm.2018.6776
MLA
Ruan, S., Deng, J., Yan, L., Huang, W."Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects". Experimental and Therapeutic Medicine 16.6 (2018): 4569-4577.
Chicago
Ruan, S., Deng, J., Yan, L., Huang, W."Evaluation of the effects of the combination of BMP‑2‑modified BMSCs and PRP on cartilage defects". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4569-4577. https://doi.org/10.3892/etm.2018.6776
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team