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Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation

  • Authors:
    • Yameng Si
    • Hanchen Chu
    • Weiwen Zhu
    • Tao Xiao
    • Xiang Shen
    • Yu Fu
    • Rongyao Xu
    • Hongbing Jiang
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, College of Stomatology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China
    Copyright: © Si et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4595-4601
    |
    Published online on: September 19, 2018
       https://doi.org/10.3892/etm.2018.6782
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Abstract

Rapamycin has been reported to be immunosuppressive and anti-proliferative towards vascular endothelial and smooth muscle cells. The purpose of the present study was to investigate the effects of rapamycin on the biological behaviors of endothelial cells that have been separated from the deformed vein in human venous malformation (VM). Cellular morphology was observed using inverted microscopy. An MTT assay was performed to measure the cell viability at different concentrations of rapamycin and different time points. Cell apoptosis and migration were detected using a terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling assay and a wound‑healing assay, respectively. At 48 and 72 h, rapamycin inhibited proliferation of human VM endothelial cells, with the effects becoming more pronounced with increasing concentration. Only rapamycin at a concentration of 1,000 ng/ml had a significant effect at 24 h in repressing proliferation. At 48 h, compared with the blank group, the majority of cells maintained a clear nuclear boundary and a regular shape following treatment with 1 ng/ml rapamycin; 10 and 100 ng/ml rapamycin caused desquamation and rounded shape; and 1,000 ng/ml rapamycin caused even more marked desquamation, rounded shape and necrosis. Rapamycin at concentrations of 1, 10, 100 and 1,000 ng/ml reduced cell viability, increased the number of apoptotic cells and suppressed the migration capacity of human VM endothelial cells, and the effects became more pronounced with increasing concentration, when compared with the blank group. These findings provide evidence that rapamycin induces apoptosis and inhibits proliferation and migration of human VM endothelial cells in a concentration‑dependent manner.
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1 

Boscolo E, Limaye N, Huang L, Kang KT, Soblet J, Uebelhoer M, Mendola A, Natynki M, Seront E, Dupont S, et al: Rapamycin improves TIE2-mutated venous malformation in murine model and human subjects. J Clin Invest. 125:3491–3504. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Jia Y, Jia J and Zhao Y: Pingyangmycin-regulated expressions of adhesion molecules in human venous malformation endothelial cells. J Huazhong Univ Sci Technolog Med. 32:760–766. 2012. View Article : Google Scholar

3 

Zheng JW, Mai HM, Zhang L, Wang YA, Fan XD, Su LX, Qin ZP, Yang YW, Jiang YH, Zhao YF and Suen JY: Guidelines for the treatment of head and neck venous malformations. Int J Clin Exp Med. 6:377–389. 2013.PubMed/NCBI

4 

Wang Y, Qi F and Gu J: Endothelial cell culture of intramuscular venous malformation and its invasive behavior related to matrix metalloproteinase-9. Plast Reconstr Surg. 123:1419–1430. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Buckmiller LM, Richter GT and Suen JY: Diagnosis and management of hemangiomas and vascular malformations of the head and neck. Oral Dis. 16:405–418. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Legiehn GM and Heran MK: Venous malformations: Classification, development, diagnosis, and interventional radiologic management. Radiol Clin North Am. 46:545–597, vi. 2008. View Article : Google Scholar : PubMed/NCBI

7 

McRae MY, Adams S, Pereira J, Parsi K and Wargon O: Venous malformations: Clinical course and management of vascular birthmark clinic cases. Australas J Dermatol. 54:22–30. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Dompmartin A, Vikkula M and Boon LM: Venous malformation: Update on aetiopathogenesis, diagnosis and management. Phlebology. 25:224–235. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Lackner H, Karastaneva A, Schwinger W, Benesch M, Sovinz P, Seidel M, Sperl D, Lanz S, Haxhija E, Reiterer F, et al: Sirolimus for the treatment of children with various complicated vascular anomalies. Eur J Pediatr. 174:1579–1584. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Glade RS, Richter GT, James CA, Suen JY and Buckmiller LM: Diagnosis and management of pediatric cervicofacial venous malformations: Retrospective review from a vascular anomalies center. Laryngoscope. 120:229–235. 2010.PubMed/NCBI

11 

Lee AB, Laredo J and Neville R: Embryological background of truncular venous malformation in the extracranial venous pathways as the cause of chronic cerebro spinal venous insufficiency. Int Angiol. 29:95–108. 2010.PubMed/NCBI

12 

Malagelada C, Jin ZH, Jackson-Lewis V, Przedborski S and Greene LA: Rapamycin protects against neuron death in in vitro and in vivo models of Parkinson's disease. The J Neurosci. 30:1166–1175. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Shafer A, Zhou C, Gehrig PA, Boggess JF and Bae-Jump VL: Rapamycin potentiates the effects of paclitaxel in endometrial cancer cells through inhibition of cell proliferation and induction of apoptosis. Int J Cancer. 126:1144–1154. 2010.PubMed/NCBI

14 

Zhao S, Huang D, Qi H, Wen J and Jia X: Comparative metabolic profiling-based improvement of rapamycin production by Streptomyces hygroscopicus. Appl Microbiol Biotechnol. 97:5329–5341. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Huber TB, Walz G and Kuehn EW: mTOR and rapamycin in the kidney: Signaling and therapeutic implications beyond immunosuppression. Kidney Int. 79:502–511. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Nichols LA, Adang LA and Kedes DH: Rapamycin blocks production of KSHV/HHV8: Insights into the anti-tumor activity of an immunosuppressant drug. PLoS One. 6:e145352011. View Article : Google Scholar : PubMed/NCBI

17 

Su W, Li Z, Jia Y and Zhuo Y: Rapamycin is neuroprotective in a rat chronic hypertensive glaucoma model. PLoS One. 9:e997192014. View Article : Google Scholar : PubMed/NCBI

18 

Lamming DW, Ye L, Katajisto P, Goncalves MD, Saitoh M, Stevens DM, Davis JG, Salmon AB, Richardson A, Ahima RS, et al: Rapamycin-induced insulin resistance is mediated by mTORC2 loss and uncoupled from longevity. Science. 335:1638–1643. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Zheng N, Ding X and Jahan R: Low concentration of rapamycin inhibits hemangioma endothelial cell proliferation, migration, and vascular tumor formation in mice. Curr Ther Res Clin Exp. 76:99–103. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Wallez Y and Huber P: Endothelial adherens and tight junctions in vascular homeostasis, inflammation and angiogenesis. Biochim Biophys Acta. 1778:794–809. 2008. View Article : Google Scholar : PubMed/NCBI

21 

DiStefano PV, Kuebel JM, Sarelius IH and Glading AJ: KRIT1 protein depletion modifies endothelial cell behavior via increased vascular endothelial growth factor (VEGF) signaling. J Biol Chem. 289:33054–33065. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Richter GT and Friedman AB: Hemangiomas and vascular malformations: Current theory and management. Int J Pediatr. 2012:6456782012. View Article : Google Scholar : PubMed/NCBI

23 

Sarbassov DD, Ali SM, Sengupta S, Sheen JH, Hsu PP, Bagley AF, Markhard AL and Sabatini DM: Prolonged rapamycin treatment inhibits mTORC2 assembly and Akt/PKB. Mol Cell. 22:159–168. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Guba M, von Breitenbuch P, Steinbauer M, Koehl G, Flegel S, Hornung M, Bruns CJ, Zuelke C, Farkas S, Anthuber M, et al: Rapamycin inhibits primary and metastatic tumor growth by antiangiogenesis: Involvement of vascular endothelial growth factor. Nat Med. 8:128–135. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Gluhovschi C, Gluhovschi G, Potencz E, Herman D, Trandafirescu V, Petrica L, Velciov S, Bozdog G, Bob F, Vernic C and Cioca D: The endothelial cell markers von Willebrand factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity. Folia Histochem Cytobiol. 48:230–236. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Mohamed AS, Thomson J, McDonald KJ, Hillyard DZ, Mark PB, Elliott HL and Jardine AG: Circulating endothelial cells in renal transplant recipients. Transpl Proc. 37:2387–2390. 2005. View Article : Google Scholar

27 

Humar R, Kiefer FN, Berns H, Resink TJ and Battegay EJ: Hypoxia enhances vascular cell proliferation and angiogenesis in vitro via rapamycin (mTOR)-dependent signaling. FASEB J. 16:771–780. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Marqués L, Núñez-Córdoba JM, Aguado L, Pretel M, Boixeda P, Nagore E, Baselga E and Redondo P: Topical rapamycin combined with pulsed dye laser in the treatment of capillary vascular malformations in Sturge-Weber syndrome: Phase II, randomized, double-blind, intraindividual placebo-controlled clinical trial. J Am Acad Dermatol. 72:151–158.e1. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Zhou C, Gehrig PA, Whang YE and Boggess JF: Rapamycin inhibits telomerase activity by decreasing the hTERT mRNA level in endometrial cancer cells. Mol Cancer Ther. 2:789–795. 2003.PubMed/NCBI

30 

Nadal M, Giraudeau B, Tavernier E, Jonville-Bera AP, Lorette G and Maruani A: Efficacy and safety of mammalian target of rapamycin inhibitors in vascular anomalies: A systematic review. Acta Derm Venereol. 96:448–452. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Shin S, Wolgamott L, Yu Y, Blenis J and Yoon SO: Glycogen synthase kinase (GSK)-3 promotes p70 ribosomal protein S6 kinase (p70S6K) activity and cell proliferation. Proc Natl Acad Sci USA. 108:E1204–E1213. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Wilkinson JE, Burmeister L, Brooks SV, Chan CC, Friedline S, Harrison DE, Hejtmancik JF, Nadon N, Strong R, Wood LK, et al: Rapamycin slows aging in mice. Aging Cell. 11:675–682. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Visner GA, Lu F, Zhou H, Liu J, Kazemfar K and Agarwal A: Rapamycin induces heme oxygenase-1 in human pulmonary vascular cells: Implications in the antiproliferative response to rapamycin. Circulation. 107:911–916. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Liu HT, Li F, Wang WY, Li XJ, Liu YM, Wang RA, Guo WY and Wang HC: Rapamycin inhibits re-endothelialization after percutaneous coronary intervention by impeding the proliferation and migration of endothelial cells and inducing apoptosis of endothelial progenitor cells. Tex Heart Inst J. 37:194–201. 2010.PubMed/NCBI

35 

Moss SC, Lightell DJ Jr, Marx SO, Marks AR and Woods TC: Rapamycin regulates endothelial cell migration through regulation of the cyclin-dependent kinase inhibitor p27Kip1. J Biol Chem. 285:11991–11997. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Shirazi F, Cohen C, Fried L and Arbiser JL: Mammalian target of rapamycin (mTOR) is activated in cutaneous vascular malformations in vivo. Lymphat Res Biol. 5:233–236. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Barilli A, Visigalli R, Sala R, Gazzola GC, Parolari A, Tremoli E, Bonomini S, Simon A, Closs EI, Dall'Asta V and Bussolati O: In human endothelial cells rapamycin causes mTORC2 inhibition and impairs cell viability and function. Cardiovasc Res. 78:563–571. 2008. View Article : Google Scholar : PubMed/NCBI

38 

Zhu W, Masaki T, Cheung AK and Kern SE: In-vitro release of rapamycin from a thermosensitive polymer for the inhibition of vascular smooth muscle cell proliferation. J Bioequiv Availab. 1:3–12. 2009.PubMed/NCBI

39 

Guo N, Chen F, Zhou J, Fang Y, Li H, Luo Y and Zhang Y: Curcumin attenuates rapamycin-induced cell injury of vascular endothelial cells. J Cardiovasc Pharmacol. 66:338–346. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Laurian C, Diner P, Enjolras O, LeMarchand-Venencie F, Herbreteau D and Merland JJ: Surgical treatment of superficial vascular malformations. Indications for tissue expansion. J Des Maladies Vasculaires. 21:31–35. 1996.(In French).

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Copy and paste a formatted citation
Spandidos Publications style
Si Y, Chu H, Zhu W, Xiao T, Shen X, Fu Y, Xu R and Jiang H: Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation. Exp Ther Med 16: 4595-4601, 2018.
APA
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y. ... Jiang, H. (2018). Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation. Experimental and Therapeutic Medicine, 16, 4595-4601. https://doi.org/10.3892/etm.2018.6782
MLA
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y., Xu, R., Jiang, H."Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation". Experimental and Therapeutic Medicine 16.6 (2018): 4595-4601.
Chicago
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y., Xu, R., Jiang, H."Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4595-4601. https://doi.org/10.3892/etm.2018.6782
Copy and paste a formatted citation
x
Spandidos Publications style
Si Y, Chu H, Zhu W, Xiao T, Shen X, Fu Y, Xu R and Jiang H: Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation. Exp Ther Med 16: 4595-4601, 2018.
APA
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y. ... Jiang, H. (2018). Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation. Experimental and Therapeutic Medicine, 16, 4595-4601. https://doi.org/10.3892/etm.2018.6782
MLA
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y., Xu, R., Jiang, H."Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation". Experimental and Therapeutic Medicine 16.6 (2018): 4595-4601.
Chicago
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y., Xu, R., Jiang, H."Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4595-4601. https://doi.org/10.3892/etm.2018.6782
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