Open Access

Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation

  • Authors:
    • Yameng Si
    • Hanchen Chu
    • Weiwen Zhu
    • Tao Xiao
    • Xiang Shen
    • Yu Fu
    • Rongyao Xu
    • Hongbing Jiang
  • View Affiliations

  • Published online on: September 19, 2018     https://doi.org/10.3892/etm.2018.6782
  • Pages: 4595-4601
  • Copyright: © Si et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rapamycin has been reported to be immunosuppressive and anti-proliferative towards vascular endothelial and smooth muscle cells. The purpose of the present study was to investigate the effects of rapamycin on the biological behaviors of endothelial cells that have been separated from the deformed vein in human venous malformation (VM). Cellular morphology was observed using inverted microscopy. An MTT assay was performed to measure the cell viability at different concentrations of rapamycin and different time points. Cell apoptosis and migration were detected using a terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling assay and a wound‑healing assay, respectively. At 48 and 72 h, rapamycin inhibited proliferation of human VM endothelial cells, with the effects becoming more pronounced with increasing concentration. Only rapamycin at a concentration of 1,000 ng/ml had a significant effect at 24 h in repressing proliferation. At 48 h, compared with the blank group, the majority of cells maintained a clear nuclear boundary and a regular shape following treatment with 1 ng/ml rapamycin; 10 and 100 ng/ml rapamycin caused desquamation and rounded shape; and 1,000 ng/ml rapamycin caused even more marked desquamation, rounded shape and necrosis. Rapamycin at concentrations of 1, 10, 100 and 1,000 ng/ml reduced cell viability, increased the number of apoptotic cells and suppressed the migration capacity of human VM endothelial cells, and the effects became more pronounced with increasing concentration, when compared with the blank group. These findings provide evidence that rapamycin induces apoptosis and inhibits proliferation and migration of human VM endothelial cells in a concentration‑dependent manner.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Si Y, Chu H, Zhu W, Xiao T, Shen X, Fu Y, Xu R and Jiang H: Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation. Exp Ther Med 16: 4595-4601, 2018
APA
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y. ... Jiang, H. (2018). Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation. Experimental and Therapeutic Medicine, 16, 4595-4601. https://doi.org/10.3892/etm.2018.6782
MLA
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y., Xu, R., Jiang, H."Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation". Experimental and Therapeutic Medicine 16.6 (2018): 4595-4601.
Chicago
Si, Y., Chu, H., Zhu, W., Xiao, T., Shen, X., Fu, Y., Xu, R., Jiang, H."Concentration‑dependent effects of rapamycin on proliferation, migration and apoptosis of endothelial cells in human venous malformation". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4595-4601. https://doi.org/10.3892/etm.2018.6782