Open Access

A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells

  • Authors:
    • Li Huang
    • Chuankong Sun
    • Ruobing Peng
    • Zhiming Liu
  • View Affiliations

  • Published online on: September 25, 2018     https://doi.org/10.3892/etm.2018.6792
  • Pages: 4367-4372
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to explore the mechanism of agonists in regulating transcriptional level of polymeric immunoglobulin receptor (pIgR) in salivary gland epithelial cells, thus revealing the defense effect of salivary immune on bacteria in the oral cavity. Sixty patients with oral bacterial infection and 70 patients suffering from oral diseases without bacterial infection were selected randomly from patients in Renmin Hospital of Wuhan University from April 2015 to April 2017. Ribonucleic acid (RNA) was extracted from salivary gland epithelial cells of all patients. Fluorescent quantitative polymerase chain reaction (FQ-PCR) and western blotting methods were adopted to detect and compare the transcriptional level of pIgR. The salivary gland epithelial cells of the 60 patients with oral bacterial infection were isolated and extracted, and they were divided into two groups (observation group and control group) randomly. Agonists were added to the observation group for acting for 24 h. FQ-PCR and immunofluorescence (IF) were adopted to detect and compare the transcriptional level of pIgR after acting with agonists. The toxicity of agonists on the cells was detected with Cell Counting kit-8 (CCK-8). The isolated salivary gland epithelial cells conformed to the morphology of epithelial cells, and adhered to the wall for growing. The transcriptional level of pIgR in the bacterial infection group was lower than that in the non‑bacterial infection group (p<0.05). The transcriptional level of pIgR in the observation group was higher than that in the control group (p<0.05) after acting with agonists. Agonists can promote the rise of transcriptional level of pIgR in salivary gland epithelial cells, and the increase in pIgR is closely related to the cure of oral bacterial infection. Therefore, agonists can improve the oral immune function by regulating the transcription of pIgR.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Huang L, Sun C, Peng R and Liu Z: A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells. Exp Ther Med 16: 4367-4372, 2018
APA
Huang, L., Sun, C., Peng, R., & Liu, Z. (2018). A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells. Experimental and Therapeutic Medicine, 16, 4367-4372. https://doi.org/10.3892/etm.2018.6792
MLA
Huang, L., Sun, C., Peng, R., Liu, Z."A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells". Experimental and Therapeutic Medicine 16.6 (2018): 4367-4372.
Chicago
Huang, L., Sun, C., Peng, R., Liu, Z."A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4367-4372. https://doi.org/10.3892/etm.2018.6792