Exenatide reduces oxidative stress and cell death in testis in iron overload rat model

Yesil,Suleyman; Sungu,Nuran; Kilicarslan,Aydan; Kuskonmaz,Serife  Mehlika; Kara,Halil; Kucuk,Aysegul; Polat,Fazli; Kavutcu,Mustafa; Arslan,Mustafa

doi:10.3892/etm.2018.6795

Exenatide reduces oxidative stress and cell death in testis in iron overload rat model

Authors:
- Suleyman Yesil
- Nuran Sungu
- Aydan Kilicarslan
- Serife Mehlika Kuskonmaz
- Halil Kara
- Aysegul Kucuk
- Fazli Polat
- Mustafa Kavutcu
- Mustafa Arslan
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Affiliations: Department of Urology, Gazi University Medical Faculty, Ankara 06510, Turkey, Department of Pathology, Yıldırım Beyazıt University Medical Faculty, Ankara 06010, Turkey, Department of Endocrinology and Metabolism, Gazi University Medical Faculty, Ankara 06510, Turkey, Department of Pharmacology, Yıldırım Beyazıt University Medical Faculty, Ankara 06010, Turkey, Department of Physiology, Kütahya Health Sciences University Medical Faculty, Kütahya 43100, Turkey, Department of Biochemistry, Gazi University Medical Faculty, Ankara 06510, Turkey, Department of Anesthesiology and Reanimation, Gazi University Medical Faculty, Ankara 06510, Turkey
Published online on: September 26, 2018 https://doi.org/10.3892/etm.2018.6795
Pages: 4349-4356
Copyright: © Yesil et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glucagon‑like peptide‑1 (GLP‑1) has been demonstrated to affect the oxidative stress status in several in vitro, in vivo and clinical studies. The aim of the present study was to evaluate the effect of a GLP‑1 analogue, exenatide, on oxidative stress parameters and apoptotic markers in testicular cells in an iron overload rat model. To obtain this model, the animals were randomly divided into three groups (n=6/group). Rats in the control group received intraperitoneal injections of saline. Intraperitoneal iron dextran (60 mg/kg/day) was given to Group FE for 5 days a week for 4 weeks. The third group (Group Fe +E) was given subcutaneous injections of 10 µg/kg exenatide in two divided doses for 4 weeks in addition to iron dextran. Testes of all rats were immediately removed for immunohistochemical staining and to measure the malondialdehyde level and superoxide dismutase enzyme activity. A significant reduction was observed in caspase‑8 and ‑3 enzyme staining in testicular stromal and endothelial cells in exenatide injected iron overloaded rats when compared with controls. Oxidative stress markers malondialdehyde levels and superoxide dismutase enzyme activities were also significantly lower in exenatide‑injected rats when compared with controls. These findings indicate that exenatide may be protective against the harmful effects of iron accumulation in testis. Further studies are required to evaluate how exenatide reduces oxidative stress and cell death in iron overloaded testis tissue.

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