MicroRNA‑106b promotes the proliferation, migration and invasion of retinoblastoma cells by inhibiting the expression of ZBTB4 protein

Bu,Wenjuan; Wang,Yanhui; Min,Xiangrong

doi:10.3892/etm.2018.6811

MicroRNA‑106b promotes the proliferation, migration and invasion of retinoblastoma cells by inhibiting the expression of ZBTB4 protein

Authors:
- Wenjuan Bu
- Yanhui Wang
- Xiangrong Min
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Affiliations: Department of Ophthalmology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Fundus Surgery, Hebei Province Eye Hospital, Xingtai, Hebei 054000, P.R. China
Published online on: October 1, 2018 https://doi.org/10.3892/etm.2018.6811
Pages: 4537-4545
Copyright: © Bu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the function of microRNA (miR)‑106b in the proliferation, migration and invasion of retinoblastoma (RB) cells, and aimed to elucidate the underlying mechanism. A total of 56 patients with RB were enrolled in the present study. The expression of miR‑106b in RB tissues was measured by reverse transcription quantitative polymerase chain reaction. After transfection with miR‑106b mimics or miR‑106b inhibitor, a Cell‑Counting kit‑8 assay was used to determine the proliferation of WERI‑Rb‑1 cells and a Transwell assay was employed to measure the migration and invasion of the cells. Western blot analysis was performed to determine the expression of zinc finger and BTB domain containing 4 (ZBTB4) protein. By silencing or overexpression of ZBTB4 protein, the biological functions of ZBTB4 in WERI‑Rb‑1 cells were studied. A dual luciferase reporter assay was performed to test whether ZBTB4 was a target gene of miR‑106b. The expression of miR‑106b in RB tissues was elevated and closely associated with the severity of the disease. Overexpression of miR‑106b increased but inhibition of miR‑106b expression decreased the proliferation, migration and invasion abilities of WERI‑Rb‑1 cells. In addition, overexpression of miR‑106b decreased but inhibition of miR‑106b expression increased ZBTB4 protein expression in WERI‑Rb‑1 cells. Similarly, overexpression of ZBTB4 reduced but inhibition of ZBTB4 expression promoted the proliferation, migration and invasion of WERI‑Rb‑1 cells. Finally, miR‑106b regulated the expression of ZBTB4 by binding to the 3'‑untranslated region of the ZBTB4 gene. The present study demonstrated that increased expression of miR‑106b in RB tissues is positively associated with the metastasis and differentiation of RB cells. As an oncogene, miR‑106b promotes the proliferation, migration and invasion of WERI‑Rb‑1 cells by inhibiting the expression of ZBTB4 protein.

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