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Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway

  • Authors:
    • Ganxian Wang
    • Guangcheng Sun
    • Yi Wang
    • Pei Yu
    • Xue Wang
    • Birong Zhou
    • Huaqing Zhu
  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, Anhui 230032, P.R. China, Department of Cardiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 107-114
    |
    Published online on: October 30, 2018
       https://doi.org/10.3892/etm.2018.6903
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Abstract

Atherosclerosis is caused by various factors, and Glabridin may have protective effects on the cardiovascular system. The purpose of the present study was to evaluate the effects of Glabridin on atherosclerosis and evaluate whether Glabridin attenuates arteriosclerosis and endothelial permeability by suppressing the myosin light chain (MLC) kinase (MLCK)/phosphorylated (p)‑MLC system via the mitogen activated protein kinase (MAPK) signaling pathway. Male New Zealand rabbits were randomly divided into 3 groups: The control group was administered an ordinary diet, whereas the high fat group and the Glabridin (2 mg/kg/d) intervention group were administered a high fat diet. Following 12 weeks, the blood lipid levels of rabbits, the morphological structure of the arterial wall, the arterial intimal permeability, the endothelial function and the mRNA levels of MLCK were measured. Western blot analysis was used to detect the levels of MLCK, p‑c‑Jun N‑terminal kinase (JNK), p‑extracellular signal regulated kinase (ERK), and p‑p38. The high‑fat diet group exhibited significantly increased total cholesterol and triglycerides, and endothelial dysfunction, which were attenuated by Glabridin treatment. Notably, the aortic endothelial permeability was increased in the high‑fat diet group but was ameliorated in the Glabridin treatment group. Hyperlipidemia enhanced the expression of p‑MLC and MLCK, which were associated with the increased phosphorylation of ERK, p38 and JNK. These changes were also ameliorated by Glabridin. In conclusion, the results of the present study suggested that atherosclerosis may be associated with upregulated MLCK expression and activity, which was downregulated by Glabridin. The mechanism of action of Glabridin was thought to proceed through modulating MAPK pathway signal transduction. However, further studies are required to adequately illuminate the exact regulatory mechanisms involved.
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Copy and paste a formatted citation
Spandidos Publications style
Wang G, Sun G, Wang Y, Yu P, Wang X, Zhou B and Zhu H: Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway. Exp Ther Med 17: 107-114, 2019.
APA
Wang, G., Sun, G., Wang, Y., Yu, P., Wang, X., Zhou, B., & Zhu, H. (2019). Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway. Experimental and Therapeutic Medicine, 17, 107-114. https://doi.org/10.3892/etm.2018.6903
MLA
Wang, G., Sun, G., Wang, Y., Yu, P., Wang, X., Zhou, B., Zhu, H."Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway". Experimental and Therapeutic Medicine 17.1 (2019): 107-114.
Chicago
Wang, G., Sun, G., Wang, Y., Yu, P., Wang, X., Zhou, B., Zhu, H."Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway". Experimental and Therapeutic Medicine 17, no. 1 (2019): 107-114. https://doi.org/10.3892/etm.2018.6903
Copy and paste a formatted citation
x
Spandidos Publications style
Wang G, Sun G, Wang Y, Yu P, Wang X, Zhou B and Zhu H: Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway. Exp Ther Med 17: 107-114, 2019.
APA
Wang, G., Sun, G., Wang, Y., Yu, P., Wang, X., Zhou, B., & Zhu, H. (2019). Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway. Experimental and Therapeutic Medicine, 17, 107-114. https://doi.org/10.3892/etm.2018.6903
MLA
Wang, G., Sun, G., Wang, Y., Yu, P., Wang, X., Zhou, B., Zhu, H."Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway". Experimental and Therapeutic Medicine 17.1 (2019): 107-114.
Chicago
Wang, G., Sun, G., Wang, Y., Yu, P., Wang, X., Zhou, B., Zhu, H."Glabridin attenuates endothelial dysfunction and permeability, possibly via the MLCK/p‑MLC signaling pathway". Experimental and Therapeutic Medicine 17, no. 1 (2019): 107-114. https://doi.org/10.3892/etm.2018.6903
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