Human amniotic membrane intra‑articular injection prevents cartilage damage in an osteoarthritis model

Marino‑Martínez,I. A.; Martínez‑Castro,A. G.; Peña‑Martínez,V. M.; Acosta‑Olivo,C. A.; Vílchez‑Cavazos,F.; Guzmán‑López,A.; Pérez Rodríguez,Edelmiro; Romero‑Díaz,V. J.; Ortega‑Blanco,J. A.; Lara‑Arias,J.

doi:10.3892/etm.2018.6924

Human amniotic membrane intra‑articular injection prevents cartilage damage in an osteoarthritis model

Authors:
- I. A. Marino‑Martínez
- A. G. Martínez‑Castro
- V. M. Peña‑Martínez
- C. A. Acosta‑Olivo
- F. Vílchez‑Cavazos
- A. Guzmán‑López
- Edelmiro Pérez Rodríguez
- V. J. Romero‑Díaz
- J. A. Ortega‑Blanco
- J. Lara‑Arias
View Affiliations

Affiliations: Pathology Service, Hospital Universitario ‘Dr. José E. González, Universidad Autónoma de Nuevo León, Monterrey, NL 64460, Mexico, Orthopedics and Traumatology Service, Hospital Universitario ‘Dr. José E. González, Universidad Autónoma de Nuevo León, Monterrey, NL 64460, Mexico, Obstetrics and Gynecology Service, Hospital Universitario ‘Dr. José E. González, Universidad Autónoma de Nuevo León, Monterrey, NL 64460, Mexico, Transplants Service, Hospital Universitario ‘Dr. José E. González, Universidad Autónoma de Nuevo León, Monterrey, NL 64460, Mexico, Department of Histology, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, NL 64460, Mexico, Top Health, SAPI de CV, Zapopan, JAL 45070, Mexico
Published online on: November 2, 2018 https://doi.org/10.3892/etm.2018.6924
Pages: 11-16
Copyright: © Marino‑Martínez et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Osteoarthritis (OA) is a degenerative joint disease that affects the soft tissues and bones of involved articulations as a result of deregulation between synthesis and extracellular matrix degradation in articular cartilage. The present study evaluated the effect of intra‑articular injection of human amniotic membrane (AM) as a treatment in an OA animal model in the knee. Chemical OA was developed in the knees of New Zealand rabbits. Once OA was established, the right knees only were treated with an intra‑articular injection of human AM, with the left knees considered as a negative control group. The evaluation was performed at 3 and 6 weeks post‑treatment. At 3 weeks post‑injection, the cartilage exhibited fibrillation, erosion, cracks and cell clusters in the negative control group, but not in the treated group (P=0.028). At 6 weeks post‑injection, the left knees exhibited hypertrophy, cracks, cell clusters, decreased matrix staining and structure loss. However, the right knees exhibited cell clusters without evidence of disruption in cartilage integrity (P=0.015). These results suggested that the intra‑articular injection of human AM delays histological changes of cartilage in OA.

View Figures

View References

1	Felson DT, Lawrence RC, Dieppe PA, Hirsch R, Helmick CG, Jordan JM, Kington RS, Lane NE, Nevitt MC, Zhang Y, et al: Osteoarthritis: New insights. Part 1 The disease and its risk factors. Ann Intern Med. 133:635–646. 2000. View Article : Google Scholar : PubMed/NCBI
2	Giménez Basallote S, Pulido Morillo FJ and Trigueros Carrero JA: Definición, etiopatogenia, factores de riesgo y pronóstico. In: Guía de Buena Práctica Clínica en Artrosis. 2nd. International Marketing & Communication, SA (IM&C); Madrid, Spain: pp. 11–17. 2008, (In Spanish).
3	Ajadi RA, Otesile EB and Kasali OB: Short-term changes in lipid profile following experimental osteoarthritis in dogs. Bulgarian J Veterinary Med. 15:166–171. 2012.
4	Anitua E, Sanchez M, Nurden AT, Zalduendo MM, de la Fuente M, Azofra J and Andía I: Platelet-released growth factors enhance the secretion of hyaluronic acid and induce hepatocyte growth factor production by synovial fibroblasts from arthritic patients. Rheumatology (Oxford). 46:1769–1772. 2007. View Article : Google Scholar : PubMed/NCBI
5	Gajiwala K and Gajiwala AL: Evaluation of lyophilized, gamma-irradiated amnion as a biological dressing. Cell Tissue Bank. 5:73–80. 2004. View Article : Google Scholar : PubMed/NCBI
6	Gruss JS and Jirsch DW: Human amniotic membrane: A versatile wound dressing. Can Med Assoc J. 118:1237–1246. 1978.PubMed/NCBI
7	Subrahmanyam M: Amniotic membrane as a cover for microskin grafts. Br J Plast Surg. 48:477–478. 1995. View Article : Google Scholar : PubMed/NCBI
8	Ward DJ, Bennett JP, Burgos H and Fabre J: The healing of chronic venous leg ulcers with prepared human amnion. Br J Plast Surg. 42:463–467. 1989. View Article : Google Scholar : PubMed/NCBI
9	Adinolfi M, Akle CA, McColl I, Fensom AH, Tansley L, Connolly P, Hsi BL, Faulk WP, Travers P and Bodmer WF: Expression of HLA antigens, beta 2-microglobulin and enzymes by human amniotic epithelial cells. Nature. 295:325–327. 1982. View Article : Google Scholar : PubMed/NCBI
10	Akle CA, Adinolfi M, Welsh KI, Leibowitz S and McColl I: Immunogenicity of human amniotic epithelial cells after transplantation into volunteers. Lancet. 2:1003–1005. 1981. View Article : Google Scholar : PubMed/NCBI
11	Faulk WP, Matthews R, Stevens PJ, Bennett JP, Burgos H and His BL: Human amnion as an adjunct in wound healing. Lancet. 1:1156–1158. 1980. View Article : Google Scholar : PubMed/NCBI
12	Kim JC and Tseng SC: Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea. 14:473–484. 1995. View Article : Google Scholar : PubMed/NCBI
13	Zohar Y, Talmi YP, Finkelstein Y, Shvili Y, Sadov R and Laurian N: Use of human amniotic membrane in otolaryngologic practice. Laryngoscope. 97:978–980. 1987. View Article : Google Scholar : PubMed/NCBI
14	Rennekampff HO, Dohrmann P, Föry R and Fändrich F: Evaluation of amniotic membrane as adhesion prophylaxis in a novel surgical gastroschisis model. J Invest Surg. 7:187–193. 1994. View Article : Google Scholar : PubMed/NCBI
15	He Q, Li Q, Chen B and Wang Z: Repair of flexor tendon defects of rabbit with tissue engineering method. Chin J Traumatol. 5:200–208. 2002.PubMed/NCBI
16	Mligiliche N, Endo K, Okamoto K, Fujimoto E and Ide C: Extracellular matrix of human amnion manufactured into tubes as conduits for peripheral nerve regeneration. J Biomed Mater Res. 63:591–600. 2002. View Article : Google Scholar : PubMed/NCBI
17	Parolini O, Alviano F, Bagnara GP, Bilic G, Buhring HJ, Evangelista M, Hennerbichler S, Liu B, Magatti M, Mao N, et al: Concise review: Isolation and characterization of cells from human term placenta: Outcome of the first international workshop on placenta derived stem cells. Stem Cells. 26:300–311. 2008. View Article : Google Scholar : PubMed/NCBI
18	Miki T, Lehmann T, Cai H, Stolz DB and Strom SC: Stem cell characteristics of amniotic epithelial cells. Stem Cells. 23:1549–1559. 2005. View Article : Google Scholar : PubMed/NCBI
19	Portmann Lanz CB, Schoeberlein A, Huber A, Sager R, Malek A, Holzgreve W and Surbek DV: Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuro regeneration. Am J Obstet Gynecol. 194:664–673. 2006. View Article : Google Scholar : PubMed/NCBI
20	Sakuragawa N, Kakinuma K, Kikuchi A, Okano H, Uchida S, Kamo I, Kobayashi M and Yokoyama Y: Human amnion mesenchyme cells express phenotypes of neuroglial progenitor cells. J Neurosci Res. 78:208–214. 2004. View Article : Google Scholar : PubMed/NCBI
21	Wolbank S, Peterbauer A, Fahrner M, Hennerbichler S, van Griensven M, Stadler G, Redl H and Gabriel C: Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: A comparison with human mesenchymal stem cells from adipose tissue. Tissue Eng. 13:1173–1183. 2007. View Article : Google Scholar : PubMed/NCBI
22	Zhao P, Ise H, Hongo M, Ota M, Konishi I and Nikaido T: Human amniotic mesenchymal cells have some characteristics of cardiomyocytes. Transplantation. 79:528–535. 2005. View Article : Google Scholar : PubMed/NCBI
23	Stadler G, Hennerbichler S, Lindenmair A, Peterbauer A, Hofer K, van Griensven M, Gabriel C, Redl H and Wolbank S: Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitro. Cytotherapy. 10:743–752. 2008. View Article : Google Scholar : PubMed/NCBI
24	Lindenmair A, Wolbank S, Stadler G, Meinl A, Peterbauer-Scherb A, Eibl J, Polin H, Gabriel C, van Griensven M and Redl H: Osteogenic differentiation of intact human amniotic membrane. Biomaterials. 31:8659–8665. 2010. View Article : Google Scholar : PubMed/NCBI
25	Kikuchi T, Sakuta T and Yamaguchi T: Intra-articular injection of collagenase induces experimental osteoarthritis in mature rabbits. Osteoarthritis Cartilage. 6:177–186. 1998. View Article : Google Scholar : PubMed/NCBI
26	Yoshioka M, Coutts RD, Amiel D and Hacker SA: Characterization of a model of osteoarthritis in the rabbit knee. Osteoarthritis Cartilage. 4:87–98. 1996. View Article : Google Scholar : PubMed/NCBI
27	Mankin HJ, Dorfman H, Lippiello L and Zarins A: Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg Am. 53:523–537. 1971. View Article : Google Scholar : PubMed/NCBI
28	Buckland J: Osteoarthritis: Blocking cartilage damage in a rat model of OA by intra-articular injection of an amniotic membrane allograft. Nat Rev Rheumatol. 10:1982014. View Article : Google Scholar : PubMed/NCBI
29	Willett NJ, Thote T, Lin AS, Moran S, Raji Y, Sridaran S, Stevens HY and Guldberg RE: Intraarticular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development. Arthritis Res Ther. 16:R472014. View Article : Google Scholar : PubMed/NCBI
30	Raines AL, Shih MS, Chua L, Su CW, Tseng SC and O'Connell J: Efficacy of particulate amniotic membrane and umbilical cord tissues in attenuating cartilage destruction in an osteoarthritis model. Tissue Eng Part A. 23:12–19. 2017. View Article : Google Scholar : PubMed/NCBI
31	Vines JB, Aliprantis AO, Gomoll AH and Farr J: Cryopreserved amniotic suspension for the treatment of knee osteoarthritis. J Knee Surg. 29:443–450. 2016. View Article : Google Scholar : PubMed/NCBI
32	Díaz-Prado S, Rendal-Vázquez ME, Muiños-López E, Hermida-Gómez T, Rodríguez-Cabarcos M, Fuentes-Boquete I, de Toro FJ and Blanco FJ: Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair. Cell Tissue Bank. 11:183–195. 2010. View Article : Google Scholar : PubMed/NCBI
33	Lucero JM, Castiglioni AE, Hovanyecz P, Gorla A, Berasategui O, Fedrigo GV and Lorenti AS: Culture of chondrocytes on an acellular matrix derived from amniochorionic membrane. Rev Asoc Argent Ortop Traumatol. 77:207–212. 2012.(In Spanish). View Article : Google Scholar
34	Bischoff M, Stachon T, Seitz B, Huber M, Zawada M, Langenbucher A and Szentmáry N: Growth factor and interleukin concentrations in amniotic membrane-conditioned medium. Curr Eye Res. 42:174–180. 2017. View Article : Google Scholar : PubMed/NCBI
35	Kim YI, Ryu JS, Yeo JE, Choi YJ, Kim YS, Ko K and Koh YG: Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells. Biochem Biophys Res Commun. 450:1593–1599. 2014. View Article : Google Scholar : PubMed/NCBI