Fluid resuscitation via the rectum ameliorates hemodynamic disorders through adjusting aquaporin expression in an experimental severe acute pancreatitis model

Xie,Rongli; Wang,Jinlong; Yao,Yi; Qi,Mengzhi; Huang,Shunwei; Zhao,Zhifeng; Chen,Ying; Yang,Zhitao; Sheng,Huiqiu; Fei,Jian; Mao,Enqiang; Chen,Erzhen

doi:10.3892/etm.2018.6934

Fluid resuscitation via the rectum ameliorates hemodynamic disorders through adjusting aquaporin expression in an experimental severe acute pancreatitis model

Authors:
- Rongli Xie
- Jinlong Wang
- Yi Yao
- Mengzhi Qi
- Shunwei Huang
- Zhifeng Zhao
- Ying Chen
- Zhitao Yang
- Huiqiu Sheng
- Jian Fei
- Enqiang Mao
- Erzhen Chen
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Affiliations: Department of General Surgery, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, Emergency Center of The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China, Department of Emergency, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China
Published online on: November 6, 2018 https://doi.org/10.3892/etm.2018.6934
Pages: 437-443

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Abstract

Acute pancreatitis is an acute abdominal disease, with 10‑20% of the cases deteriorating rapidly, accompanied by persistent organ failure and further development into severe acute pancreatitis (SAP). The aim of the present study was to investigate the mechanism of fluid resuscitation via the rectum in the early stages of SAP and the role of aquaporins (AQPs). An SAP model was constructed by injection of 5% sterile sodium taurocholate into the biliopancreatic duct of Sprague Dawley rats, and the mean arterial pressure (MAP) was continuously monitored via femoral artery catheterization. At 30 min after the construction of the SAP model, the rats in the fluid resuscitation groups were resuscitated with normal saline at a rate of 4 ml/kg/h through the venous or the rectal route. The AQP and Na+‑K+‑ATPase levels, and the correlation of the MAP and colon AQPs at the early stages of SAP were analyzed. The results demonstrated that the mRNA level of AQP‑3 and AQP‑4 in the distal colon decreased significantly in the group subjected to fluid resuscitation via the rectum, while no significant differences were identified in the Na+‑K+‑ATPase levels of the colon in that group. Furthermore, a negative correlation was identified between the expression of AQPs and the MAP (P<0.01). Thus, fluid resuscitation via the rectum appears to ameliorate hemodynamic disorders through adjusting the expression of AQP‑3 and AQP‑4 in the distal colon in an experimental SAP model.

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