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Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study

  • Authors:
    • Darian Rusu
    • Marius Boariu
    • Ștefan-Ioan Stratul
    • Florina Bojin
    • Virgil Paunescu
    • Horia Calniceanu
    • Petra Surlin
    • Alexandra Roman
    • Ștefan Milicescu
    • Costin Caruntu
    • Andreea Didilescu
    • Nela-Pusa Gaje
    • Bogdan Calenic
  • View Affiliations / Copyright

    Affiliations: Department of Periodontology, ‘Victor Babes’ University of Medicine and Pharmacy, 300141 Timisoara, Romania, Department of Restorative Dentistry and Endodontics, ‘Victor Babes’ University of Medicine and Pharmacy, 300141 Timisoara, Romania, Department of Physiology, ‘Victor Babes’ University of Medicine and Pharmacy, 300230 Timisoara, Romania, Department of Periodontology, University of Medicine and Pharmacy, 200349 Craiova, Romania, Department of Periodontology, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania, Department of Fixed Prosthodontics, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania, Department of Physiology, Faculty of Midwifery and Nursing, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania, Department of Embryology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania, Department of Histology, ‘Victor Babes’ University of Medicine and Pharmacy, 300230 Timisoara, Romania, Department of Biochemistry, Faculty of Dental Medicine, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania
    Copyright: © Rusu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 990-996
    |
    Published online on: November 16, 2018
       https://doi.org/10.3892/etm.2018.6979
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Abstract

Previous experimental models showed that activation of the immune system, particularly T cells, is required for optimal healing following wounds or surgery in the oral cavity. Therefore, studies to explore the interactions between the immune system and the collagen matrix are mandated. The specific aim of the present study was to analyze the interactions between T lymphocytes and a resorbable three-dimensional (3D) collagen matrix routinely used for soft tissue regeneration during periodontal surgery. Peripheral venous blood samples were collected from five patients. Following Ficoll-Paque separation, mononuclear cells were grown on fully resorbable 3D collagen matrices for 5 days. Lymphocytes were analyzed by flow cytometry for different surface markers, including CD4, CD8, CD38 and CD69. Cell viability and late apoptosis/necrosis were assessed in each group using an apoptosis assay based on Annexin V/propidium iodide staining. After 5 days in contact with the collagen matrix, the T cells expressed different surface markers. The overall T cell population increased significantly in the collagen matrix group compared to the respective controls (31.9±6.5 vs. 38.7±3.8%). CD8 and CD69 also increased significantly compared to their controls (CD69: 19.7±3.0 vs. 27.1±4.5% for collagen vs. control groups). At the same time, CD4 and CD38 expression was similar in both groups. Viability and apoptosis/necrosis were also identical in the samples and controls. These results show that the interaction between the collagen matrix and the immune cells stimulated activation of T cells and did not impair the healing process.
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1 

Nevins M, Nevins ML, Kim SW, Schupbach P and Kim DM: The use of mucograft collagen matrix to augment the zone of keratinized tissue around teeth: A pilot study. Int J Periodontics Restorative Dent. 31:367–373. 2011.PubMed/NCBI

2 

Lin GH, Chan HL and Wang HL: The significance of keratinized mucosa on implant health: A systematic review. J Periodontol. 84:1755–1767. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Hall WB and Lundergan WP: Free gingival grafts. Current indications and techniques. Dent Clin North Am. 37:227–242. 1993.PubMed/NCBI

4 

Tonetti MS and Jepsen S: Working Group 2 of the European Workshop on Periodontology: Clinical efficacy of periodontal plastic surgery procedures: Consensus report of Group 2 of the 10th European Workshop on Periodontology. J Clin Periodontol. 41 Suppl 15:S36–S43. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Zuhr O, Bäumer D and Hürzeler M: The addition of soft tissue replacement grafts in plastic periodontal and implant surgery: Critical elements in design and execution. J Clin Periodontol. 41 Suppl 15:S123–S142. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Scarano A, Barros RR, Iezzi G, Piattelli A and Novaes AB Jr: Acellular dermal matrix graft for gingival augmentation: A preliminary clinical, histologic, and ultrastructural evaluation. J Periodontol. 80:253–259. 2009. View Article : Google Scholar : PubMed/NCBI

7 

McGuire MK, Scheyer ET, Nunn ME and Lavin PT: A pilot study to evaluate a tissue-engineered bilayered cell therapy as an alternative to tissue from the palate. J Periodontol. 79:1847–1856. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Sanz M, Lorenzo R, Aranda JJ, Martin C and Orsini M: Clinical evaluation of a new collagen matrix (Mucograft prototype) to enhance the width of keratinized tissue in patients with fixed prosthetic restorations: A randomized prospective clinical trial. J Clin Periodontol. 36:868–876. 2009. View Article : Google Scholar : PubMed/NCBI

9 

McGuire MK and Scheyer ET: Randomized, controlled clinical trial to evaluate a xenogeneic collagen matrix as an alternative to free gingival grafting for oral soft tissue augmentation. J Periodontol. 85:1333–1341. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Teller P and White TK: The physiology of wound healing: Injury through maturation. Surg Clin North Am. 89:599–610. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Portou MJ, Baker D, Abraham D and Tsui J: The innate immune system, Toll-like receptors and dermal wound healing: A review. Vascul Pharmacol. 71:31–36. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Dasu MR and Isseroff RR: Toll-like receptors in wound healing: Location, accessibility, and timing. J Invest Dermatol. 132:1955–1958. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Sorg H, Tilkorn DJ, Hager S, Hauser J and Mirastschijski U: Skin wound healing: An update on the current knowledge and concepts. Eur Surg Res. 58:81–94. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Sun BK, Siprashvili Z and Khavari PA: Advances in skin grafting and treatment of cutaneous wounds. Science. 346:941–945. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Rusu D, Stratul SI, Festila D, Surlin P, Kasaj A, Baderca F, Boariu M, Jentsch H, Locovei C and Calenic B: Histology and surface ultrastructure during early healing after gingival augmentation with a three-dimensional collagen matrix: A report of six cases. Quintessence Int. 48:57–67. 2017.PubMed/NCBI

16 

Martin P and Nunan R: Cellular and molecular mechanisms of repair in acute and chronic wound healing. Br J Dermatol. 173:370–378. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Kabashima K: Basic and clinical sciences in skin immune responses. Immunology of the Skin. 1st. Springer; Japan: pp. 95–111. 2016

18 

Zhang C, Xiao C, Dang E, Cao J, Zhu Z, Fu M, Yao X, Liu Y, Jin B, Wang G, et al: CD100-Plexin-B2 promotes the inflammation in psoriasis by activating NF-κB and inflammasome in keratinocytes. J Invest Dermatol. 138:375–383. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Chen L and Flies DB: Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat Rev Immunol. 13:227–242. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Sena LA, Li S, Jairaman A, Prakriya M, Ezponda T, Hildeman DA, Wang CR, Schumacker PT, Licht JD, Perlman H, et al: Mitochondria are required for antigen-specific T cell activation through reactive oxygen species signaling. Immunity. 38:225–236. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Mackay LK, Braun A, Macleod BL, Collins N, Tebartz C, Bedoui S, Carbone FR and Gebhardt T: Cutting edge: CD69 interference with sphingosine-1-phosphate receptor function regulates peripheral T cell retention. J Immunol. 194:2059–2063. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Etich J, Bergmeier V, Frie C, Kreft S, Bengestrate L, Eming S, Mauch C, Eckes B, Ulus H, Lund FE, et al: PECAM1(+)/Sca1(+)/CD38(+) vascular cells transform into myofibroblast-like cells in skin wound repair. PLoS One. 8:e532622013. View Article : Google Scholar : PubMed/NCBI

23 

De Obaldia ME and Bhandoola A: Transcriptional regulation of innate and adaptive lymphocyte lineages. Annu Rev Immunol. 33:607–642. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Chen L, Mehta ND, Zhao Y and DiPietro LA: Absence of CD4 or CD8 lymphocytes changes infiltration of inflammatory cells and profiles of cytokine expression in skin wounds, but does not impair healing. Exp Dermatol. 23:189–194. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Nosbaum A, Prevel N, Truong HA, Mehta P, Ettinger M, Scharschmidt TC, Ali NH, Pauli ML, Abbas AK and Rosenblum MD: Cutting edge: Regulatory T cells facilitate cutaneous wound healing. J Immunol. 196:2010–2014. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Sugimoto MA, Vago JP, Teixeira MM and Sousa LP: Annexin A1 and the resolution of inflammation: Modulation of neutrophil recruitment, apoptosis, and clearance. J Immunol Res. 2016.82392582016.doi: 10.1155/2016/8239258. PubMed/NCBI

27 

Yeh SH, Kong FL and Lin MH: Visualization of apoptosis: Annexin V imaging. Personalized Pathway-Activated Systems Imaging in Oncology. Inoue T, Yang D and Huang G: Springer; Singapore: pp. 233–243. 2017, View Article : Google Scholar

28 

Pietkiewicz S, Schmidt JH and Lavrik IN: Quantification of apoptosis and necroptosis at the single cell level by a combination of imaging flow cytometry with classical Annexin V/propidium iodide staining. J Immunol Methods. 423:99–103. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Weyd H, Abeler-Dörner L, Linke B, Mahr A, Jahndel V, Pfrang S, Schnölzer M, Falk CS and Krammer PH: Annexin A1 on the surface of early apoptotic cells suppresses CD8+ T cell immunity. PLoS One. 8:e624492013. View Article : Google Scholar : PubMed/NCBI

30 

Boda D: Cellomics as integrative omics for cancer. Curr Proteomics. 10:237–245. 2013. View Article : Google Scholar

31 

Caruntu C, Boda D, Constantin C, Caruntu A and Neagu M: Catecholamines increase in vitro proliferation of murine B16F10 melanoma cells. Acta Endocrinol (Copenh). 10:545–558. 2014.

32 

Lupu M, Caruntu A, Caruntu C, Papagheorghe LM, Ilie MA, Voiculescu V, Boda D, Constantin C, Tanase C, Sifaki M, et al: Neuroendocrine factors: The missing link in non-melanoma skin cancer (Review). Oncol Rep. 38:1327–1340. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Ion A, Popa IM, Papagheorghe LM, Lisievici C, Lupu M, Voiculescu V, Caruntu C and Boda D: Proteomic approaches to biomarker discovery in cutaneous T-cell lymphoma. Dis Markers. 2016.96024722016.doi: 10.1155/2016/9602472. PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Rusu D, Boariu M, Stratul Ș, Bojin F, Paunescu V, Calniceanu H, Surlin P, Roman A, Milicescu Ș, Caruntu C, Caruntu C, et al: Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study. Exp Ther Med 17: 990-996, 2019.
APA
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H. ... Calenic, B. (2019). Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study. Experimental and Therapeutic Medicine, 17, 990-996. https://doi.org/10.3892/etm.2018.6979
MLA
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H., Surlin, P., Roman, A., Milicescu, Ș., Caruntu, C., Didilescu, A., Gaje, N., Calenic, B."Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study". Experimental and Therapeutic Medicine 17.2 (2019): 990-996.
Chicago
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H., Surlin, P., Roman, A., Milicescu, Ș., Caruntu, C., Didilescu, A., Gaje, N., Calenic, B."Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study". Experimental and Therapeutic Medicine 17, no. 2 (2019): 990-996. https://doi.org/10.3892/etm.2018.6979
Copy and paste a formatted citation
x
Spandidos Publications style
Rusu D, Boariu M, Stratul Ș, Bojin F, Paunescu V, Calniceanu H, Surlin P, Roman A, Milicescu Ș, Caruntu C, Caruntu C, et al: Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study. Exp Ther Med 17: 990-996, 2019.
APA
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H. ... Calenic, B. (2019). Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study. Experimental and Therapeutic Medicine, 17, 990-996. https://doi.org/10.3892/etm.2018.6979
MLA
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H., Surlin, P., Roman, A., Milicescu, Ș., Caruntu, C., Didilescu, A., Gaje, N., Calenic, B."Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study". Experimental and Therapeutic Medicine 17.2 (2019): 990-996.
Chicago
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H., Surlin, P., Roman, A., Milicescu, Ș., Caruntu, C., Didilescu, A., Gaje, N., Calenic, B."Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study". Experimental and Therapeutic Medicine 17, no. 2 (2019): 990-996. https://doi.org/10.3892/etm.2018.6979
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