Open Access

Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study

  • Authors:
    • Darian Rusu
    • Marius Boariu
    • Ștefan-Ioan Stratul
    • Florina Bojin
    • Virgil Paunescu
    • Horia Calniceanu
    • Petra Surlin
    • Alexandra Roman
    • Ștefan Milicescu
    • Costin Caruntu
    • Andreea Didilescu
    • Nela-Pusa Gaje
    • Bogdan Calenic
  • View Affiliations

  • Published online on: November 16, 2018     https://doi.org/10.3892/etm.2018.6979
  • Pages: 990-996
  • Copyright: © Rusu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous experimental models showed that activation of the immune system, particularly T cells, is required for optimal healing following wounds or surgery in the oral cavity. Therefore, studies to explore the interactions between the immune system and the collagen matrix are mandated. The specific aim of the present study was to analyze the interactions between T lymphocytes and a resorbable three-dimensional (3D) collagen matrix routinely used for soft tissue regeneration during periodontal surgery. Peripheral venous blood samples were collected from five patients. Following Ficoll-Paque separation, mononuclear cells were grown on fully resorbable 3D collagen matrices for 5 days. Lymphocytes were analyzed by flow cytometry for different surface markers, including CD4, CD8, CD38 and CD69. Cell viability and late apoptosis/necrosis were assessed in each group using an apoptosis assay based on Annexin V/propidium iodide staining. After 5 days in contact with the collagen matrix, the T cells expressed different surface markers. The overall T cell population increased significantly in the collagen matrix group compared to the respective controls (31.9±6.5 vs. 38.7±3.8%). CD8 and CD69 also increased significantly compared to their controls (CD69: 19.7±3.0 vs. 27.1±4.5% for collagen vs. control groups). At the same time, CD4 and CD38 expression was similar in both groups. Viability and apoptosis/necrosis were also identical in the samples and controls. These results show that the interaction between the collagen matrix and the immune cells stimulated activation of T cells and did not impair the healing process.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Rusu D, Boariu M, Stratul Ș, Bojin F, Paunescu V, Calniceanu H, Surlin P, Roman A, Milicescu Ș, Caruntu C, Caruntu C, et al: Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study. Exp Ther Med 17: 990-996, 2019
APA
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H. ... Calenic, B. (2019). Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study. Experimental and Therapeutic Medicine, 17, 990-996. https://doi.org/10.3892/etm.2018.6979
MLA
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H., Surlin, P., Roman, A., Milicescu, Ș., Caruntu, C., Didilescu, A., Gaje, N., Calenic, B."Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study". Experimental and Therapeutic Medicine 17.2 (2019): 990-996.
Chicago
Rusu, D., Boariu, M., Stratul, Ș., Bojin, F., Paunescu, V., Calniceanu, H., Surlin, P., Roman, A., Milicescu, Ș., Caruntu, C., Didilescu, A., Gaje, N., Calenic, B."Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study". Experimental and Therapeutic Medicine 17, no. 2 (2019): 990-996. https://doi.org/10.3892/etm.2018.6979