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Article

Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats

  • Authors:
    • Xiangwan Sun
    • Ying Gao
    • Xin Wang
    • Ge Hu
    • Yun Wang
    • Bo Feng
    • Yanxin Hu
    • Xiang Mu
    • Ying Zhang
    • Hong Dong
  • View Affiliations / Copyright

    Affiliations: Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R. China, Department of Biology, Tennessee Center for Botanical Medicine Research, Middle Tennessee State University, Murfreesboro, TN 37132‑0001, USA, College of Veterinary Medicine, China Agricultural University, Beijing 100083, P.R. China, School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China
  • Pages: 824-834
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    Published online on: November 20, 2018
       https://doi.org/10.3892/etm.2018.6997
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Abstract

Enterotoxigenic Escherichia coli (ETEC)‑induced diarrhea is a devastating disease and one of the third leading causes of infectious disease‑associated mortalities worldwide. Despite recent advances in the identification of the association between gut microbiota and diarrhea, a lack of understanding exists on the status of gut microbiota in rats treated with ETEC. In the present study, a rat model of Escherichia (E.) coli O101‑induced diarrhea was established. The diarrhea incidence and index, as well as histological changes, were assessed. In addition, Illumina MiSeq sequencing of V3‑V4 hypervariable regions of 16S ribosomal RNA was employed to investigate the changes in the gut microbiota profiles in the feces of the diarrhea rats. The results indicated that E. coli O101 increased the diarrhea index and injury in the intestinal tissues, whereas it decreased the bacterial richness and shifted the distribution pattern of the bacterial communities in the phylum, order and genus levels in the fecal samples. Notably, the proportion of bacteria Prevotella, Enterococcus and Akkermansia was significantly decreased, while the pathogenic bacteria Escherichia/Shigella were significantly increased in diarrhea rats. Taken together, the gut microbiota is closely associated with E. coli O101‑induced diarrhea in lower microbial diversity and dysbiosis of gut microbiota at different taxonomical levels.
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Copy and paste a formatted citation
Spandidos Publications style
Sun X, Gao Y, Wang X, Hu G, Wang Y, Feng B, Hu Y, Mu X, Zhang Y, Dong H, Dong H, et al: Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats. Exp Ther Med 17: 824-834, 2019.
APA
Sun, X., Gao, Y., Wang, X., Hu, G., Wang, Y., Feng, B. ... Dong, H. (2019). Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats. Experimental and Therapeutic Medicine, 17, 824-834. https://doi.org/10.3892/etm.2018.6997
MLA
Sun, X., Gao, Y., Wang, X., Hu, G., Wang, Y., Feng, B., Hu, Y., Mu, X., Zhang, Y., Dong, H."Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats". Experimental and Therapeutic Medicine 17.1 (2019): 824-834.
Chicago
Sun, X., Gao, Y., Wang, X., Hu, G., Wang, Y., Feng, B., Hu, Y., Mu, X., Zhang, Y., Dong, H."Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats". Experimental and Therapeutic Medicine 17, no. 1 (2019): 824-834. https://doi.org/10.3892/etm.2018.6997
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Spandidos Publications style
Sun X, Gao Y, Wang X, Hu G, Wang Y, Feng B, Hu Y, Mu X, Zhang Y, Dong H, Dong H, et al: Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats. Exp Ther Med 17: 824-834, 2019.
APA
Sun, X., Gao, Y., Wang, X., Hu, G., Wang, Y., Feng, B. ... Dong, H. (2019). Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats. Experimental and Therapeutic Medicine, 17, 824-834. https://doi.org/10.3892/etm.2018.6997
MLA
Sun, X., Gao, Y., Wang, X., Hu, G., Wang, Y., Feng, B., Hu, Y., Mu, X., Zhang, Y., Dong, H."Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats". Experimental and Therapeutic Medicine 17.1 (2019): 824-834.
Chicago
Sun, X., Gao, Y., Wang, X., Hu, G., Wang, Y., Feng, B., Hu, Y., Mu, X., Zhang, Y., Dong, H."Escherichia coli O101‑induced diarrhea develops gut microbial dysbiosis in rats". Experimental and Therapeutic Medicine 17, no. 1 (2019): 824-834. https://doi.org/10.3892/etm.2018.6997
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