Curcumin improves perfusion recovery in experimental peripheral arterial disease by upregulating microRNA‑93 expression

Zhang,Jinfeng; Wang,Qiongtao; Rao,Guotao; Qiu,Junying; He,Ronghua

doi:10.3892/etm.2018.7000

Curcumin improves perfusion recovery in experimental peripheral arterial disease by upregulating microRNA‑93 expression

Authors:
- Jinfeng Zhang
- Qiongtao Wang
- Guotao Rao
- Junying Qiu
- Ronghua He
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Affiliations: Department of Cardiology, The Central Hospital of Xiaogan, Wuhan University of Science and Technology, Xiaogan, Hubei 432000, P.R. China
Published online on: November 21, 2018 https://doi.org/10.3892/etm.2018.7000
Pages: 798-802
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In peripheral arterial disease (PAD), angiogenesis is the major process involved in repairing the microvasculature in the ischemic lower limb. Curcumin, a monomer isolated from turmeric roots, has been demonstrated to have pro‑ and anti‑angiogenic effects under different circumstances. Previous studies have indicated that curcumin treatment improves tissue repair and perfusion recovery in a mouse model of diabetic PAD. However, the effects of curcumin on PAD under non‑diabetic conditions has remained elusive, In the present study, mice with PAD and a normal glycaemic profile were treated with curcumin, which improved perfusion recovery, increased capillary density and elevated microRNA (miR)‑93 expression in ischemic muscle tissue. In cultured endothelial cells under simulated ischemia, curcumin improved endothelial cell viability and enhanced tube formation. However, following miR‑93 knockdown using a microRNA inhibitor, endothelial cell tube formation was inhibited. Furthermore, in the presence of the miR‑93 inhibitor, curcumin did not alter endothelial cell viability or tube formation. These results demonstrate that curcumin had beneficial effects in non‑diabetic PAD by improving angiogenesis, which may have been achieved partially via the promotion of miR‑93 expression.

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1	Guerchet M, Aboyans V, Mbelesso P, Mouanga AM, Salazar J, Bandzouzi B, Tabo A, Clément JP, Preux PM and Lacroix P: Epidemiology of peripheral artery disease in elder general population of two cities of Central Africa: Bangui and Brazzaville. Eur J Vasc Endovasc Surg. 44:164–169. 2012. View Article : Google Scholar : PubMed/NCBI
2	Criqui MH and Aboyans V: Epidemiology of peripheral artery disease. Circ Res. 116:1509–1526. 2015. View Article : Google Scholar : PubMed/NCBI
3	Fowkes FG, Aboyans V, Fowkes FJ, McDermott MM, Sampson UK and Criqui MH: Peripheral artery disease: Epidemiology and global perspectives. Nat Rev Cardiol. 14:156–170. 2017. View Article : Google Scholar : PubMed/NCBI
4	Espinola-Klein C and Savvidis S: Peripheral arterial disease. Epidemiology, symptoms and diagnosis. Internist (Berl). 50:919–926. 2009.(In German). View Article : Google Scholar : PubMed/NCBI
5	Grundmann S, Piek JJ, Pasterkamp G and Hoefer IE: Arteriogenesis: Basic mechanisms and therapeutic stimulation. Eur J Clin Invest. 37:755–766. 2007. View Article : Google Scholar : PubMed/NCBI
6	Hirsch AT: Treatment of peripheral arterial disease-extending ‘intervention’ to ‘therapeutic choice’. N Engl J Med. 354:1944–1947. 2006. View Article : Google Scholar : PubMed/NCBI
7	Joh JH, Joo SH and Park HC: Simultaneous hybrid revascularization for symptomatic lower extremity arterial occlusive disease. Exp Ther Med. 7:804–810. 2014. View Article : Google Scholar : PubMed/NCBI
8	Aviles RJ, Annex BH and Lederman RJ: Testing clinical therapeutic angiogenesis using basic fibroblast growth factor (FGF-2). Brit J Pharmacol. 140:637–646. 2003. View Article : Google Scholar
9	Owens CD and Conte MS: Medical management of peripheral arterial disease: Bridging the ‘Gap’? Circulation. 126:1319–1321. 2012. View Article : Google Scholar : PubMed/NCBI
10	Annex BH: Therapeutic angiogenesis for critical limb ischaemia. Nat Rev Cardiol. 10:387–396. 2013. View Article : Google Scholar : PubMed/NCBI
11	Allegra A, Innao V, Russo S, Gerace D, Alonci A and Musolino C: Anticancer activity of curcumin and its analogues: Preclinical and clinical studies. Cancer Invest. 35:1–22. 2017. View Article : Google Scholar : PubMed/NCBI
12	Goozee KG, Shah TM, Sohrabi HR, Rainey-Smith SR, Brown B, Verdile G and Martins RN: Examining the potential clinical value of curcumin in the prevention and diagnosis of Alzheimer's disease. Br J Nutr. 115:449–465. 2016. View Article : Google Scholar : PubMed/NCBI
13	Jiménez-Osorio AS, Monroy A and Alavez S: Curcumin and insulin resistance-Molecular targets and clinical evidences. Biofactors. 42:561–580. 2016. View Article : Google Scholar : PubMed/NCBI
14	You J, Sun J, Ma T, Yang Z, Wang X, Zhang Z, Li J, Wang L, Ii M, Yang J and Shen Z: Curcumin induces therapeutic angiogenesis in a diabetic mouse hindlimb ischemia model via modulating the function of endothelial progenitor cells. Stem Cell Res Ther. 8:1822017. View Article : Google Scholar : PubMed/NCBI
15	Barrett PM, Wall CAM and Stack AG: Peripheral artery disease prevalence and mortality trends of United States dialysis population: 1995–2005. Irish J Med Sci. 179:S409–S410. 2010.
16	Waters RE, Terjung RL, Peters KG and Annex BH: Preclinical models of human peripheral arterial occlusive disease: Implications for investigation of therapeutic agents. J Appl Physiol (1985). 97:773–780. 2004. View Article : Google Scholar : PubMed/NCBI
17	Albadawi H, Oklu R, Cormier NR, O'Keefe RM, Heaton JT, Kobler JB, Austen WG and Watkins MT: Hind limb ischemia-reperfusion injury in diet-induced obese mice. J Surg Res. 190:683–691. 2014. View Article : Google Scholar : PubMed/NCBI
18	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
19	Baudin B, Bruneel A, Bosselut N and Vaubourdolle M: A protocol for isolation and culture of human umbilical vein endothelial cells. Nat Protoc. 2:481–485. 2007. View Article : Google Scholar : PubMed/NCBI
20	Hazarika S, Farber CR, Dokun AO, Pitsillides AN, Wang T, Lye RJ and Annex BH: MicroRNA-93 controls perfusion recovery after hindlimb ischemia by modulating expression of multiple genes in the cell cycle pathway. Circulation. 127:1818–1828. 2013. View Article : Google Scholar : PubMed/NCBI
21	Elavarasu S, Suthanthiran T, Thangavelu A, Alex S, Palanisamy VK and Kumar TS: Evaluation of superoxide dismutase levels in local drug delivery system containing 0.2% curcumin strip as an adjunct to scaling and root planing in chronic periodontitis: A clinical and biochemical study. J Pharm Bioallied Sci. 8 Suppl 1:S48–S52. 2016.PubMed/NCBI
22	Huang F, Yao Y, Wu J, Liu Q, Zhang J, Pu X, Zhang Q and Xia L: Curcumin inhibits gastric cancer-derived mesenchymal stem cells mediated angiogenesis by regulating NF-kB/VEGF signaling. Am J Transl Res. 9:5538–5547. 2017.PubMed/NCBI
23	He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI
24	González-Duarte RJ, Cázares-Ordoñez V and Ávila-Chávez E: The microRNA biogenesis machinery: Regulation by steroid hormones and alterations in cancer. Rev Invest Clin. 66:460–464. 2014.PubMed/NCBI
25	Ha M and Kim VN: Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol. 15:509–524. 2014. View Article : Google Scholar : PubMed/NCBI
26	Jonas S and Izaurralde E: Towards a molecular understanding of microRNA-mediated gene silencing. Nat Rev Genet. 16:421–433. 2015. View Article : Google Scholar : PubMed/NCBI
27	Zhou X, Yuan P and He Y: Role of microRNAs in peripheral artery disease (review). Mol Med Rep. 6:695–700. 2012. View Article : Google Scholar : PubMed/NCBI
28	Ganta VC, Choi MH, Kutateladze A, Fox TE, Farber CR and Annex BH: A MicroRNA93-interferon regulatory factor-9-immunoresponsive gene-1-itaconic acid pathway modulatesM2-like macrophage polarization to revascularize ischemic muscle. Circulation. 135:2403–2425. 2017. View Article : Google Scholar : PubMed/NCBI