miR‑96 promotes collagen deposition in keloids by targeting Smad7

Chao,Li; Hua‑Yu,Zhu; Wen‑Dong,Bai; Mei,Song; Bin,Xiao; Da‑Hai,Hu; Yi,Liu

doi:10.3892/etm.2018.7008

miR‑96 promotes collagen deposition in keloids by targeting Smad7

Authors:
- Li Chao
- Zhu Hua‑Yu
- Bai Wen‑Dong
- Song Mei
- Xiao Bin
- Hu Da‑Hai
- Liu Yi
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Affiliations: Burns and Plastic Surgery Center of People's Liberation Army, Lanzhou General Hospital of Chinese People's Liberation Army, Lanzhou, Gansu 730050, P.R. China, Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Hematology, Urumqi General Hospital of Chinese People's Liberation Army, Urumqi, Xinjiang 830000, P.R. China
Published online on: November 23, 2018 https://doi.org/10.3892/etm.2018.7008
Pages: 773-781
Copyright: © Chao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The abnormal upregulation of transforming growth factor‑β (TGF‑β) signaling has been demonstrated to initiate keloid formation and progression. Keloid is a type of benign skin tumor that may occur following sustaining skin injury. microRNA‑96 (miR‑96) serves an important role in the progression of various malignant diseases. Using reverse transcription quantitative polymerase chain reaction (RT‑qPCR), the present study demonstrated that miR‑96 was overexpressed in keloid‑derived fibroblasts (KFs). Luciferase reporter assay revealed mothers against decapentaplegic homolog (Smad)7, which is one of the important inhibitory factors in the TGF‑β pathway, as a direct target of miR‑96. miR‑96 was initially observed to be correlated with the deposition of type I collagen in KFs in vitro. The miR‑96 antagomir, was directly added into the keloid organ culture (OC) to find its significant antifibrotic potential, such as keloid OC shrinkage, exhibited by its dry weight loss and improved dermis architecture, exhibited by Masson's staining. Following miR‑96 antagomir treatment, a reduction in the mRNA and protein expression levels of collagen type I α 1 chain and collagen type 3 α 1 chain within keloid OC tissues was observed. The present study revealed that miR‑96 serves an important role in pathogenic keloid formation, suggesting that miR‑96 antagomir has the potential to prevent keloid progression.

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