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Article

lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861

  • Authors:
    • Fukun Chen
    • Shuting Yin
    • Jialun Zhu
    • Pengjie Liu
    • Chuanzhou Yang
    • Zhiping Feng
    • Zhiyong Deng
  • View Affiliations / Copyright

    Affiliations: Department of Nuclear Medicine, Yunnan Tumor Hospital, Kunming, Yunnan 650118, P.R. China, Third Ward of The Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650000, P.R. China
  • Pages: 895-900
    |
    Published online on: November 26, 2018
       https://doi.org/10.3892/etm.2018.7012
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Abstract

A vast amount of evidence indicates that long non‑coding RNAs (lncRNAs) are involved in cancer. Previous studies have indicated that lncRNA DiGeorge syndrome critical region gene 5 (DGCR5) is aberrantly expressed in lung cancer, pancreatic ductal adenocarcinoma and hepatocellular carcinoma. However, the role of DGCR5 in papillary thyroid carcinoma (PTC) has remained elusive. In the present study, it was revealed that DGCR5 was significantly downregulated in PTC tissues compared with that in adjacent normal tissues. Through functional experiments, it was demonstrated that ectopic overexpression of DGCR5 markedly suppressed PTC cell growth and invasion. A bioinformatics analysis suggested that DGCR5 binds to microRNA (miR)‑2861. A total of 5 putative binding sites for miR‑2861 were identified in DGCR5, and a luciferase reporter assay confirmed the direct interaction between DGCR5 and miR‑2861. Furthermore, reverse transcription‑quantitative polymerase chain reaction analysis indicated that ectopic overexpression of DGCR5 led to a decreased expression of miR‑2861 in PTC cells and miR‑2861 mimic transfection caused a downregulation of DGCR5. miR‑2861 level was upregulated in PTC tissues compared with adjacent tissues and negatively correlated with DGCR5 level. In addition, rescue experiments indicated that ectopic expression of miR‑2861 reversed the effects of DGCR5 overexpression on PTC cell proliferation and invasion. Taken together, the present results demonstrated that DGCR5 inhibits PTC progression via sponging miR‑2861, indicating DGCR5 may serve as a therapeutic target.
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1 

Chen AY, Jemal A and Ward EM: Increasing incidence of differentiated thyroid cancer in the United States, 1988-2005. Cancer. 115:3801–3807. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Liang L, Xu J, Wang M, Xu G, Zhang N, Wang G and Zhao Y: LncRNA HCP5 promotes follicular thyroid carcinoma progression via miRNAs sponge. Cell Death Dis. 9:3722018. View Article : Google Scholar : PubMed/NCBI

3 

Gimm O: Thyroid cancer. Cancer Lett. 163:143–156. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Hundahl SA, Fleming ID, Fremgen AM and Menck HR: A National Cancer Data Base report on 53,856 cases of thyroid carcinoma treated in the U.S., 1985-1995 [see commetns]. Cancer. 83:2638–2648. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Liu B, Ye B, Yang L, Zhu X, Huang G, Zhu P, Du Y, Wu J, Qin X, Chen R, et al: Long noncoding RNA lncKdm2b is required for ILC3 maintenance by initiation of Zfp292 expression. Nat Immunol. 18:499–508. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Zhu P, Wang Y, Wu J, Huang G, Liu B, Ye B, Du Y, Gao G, Tian Y, He L and Fan Z: LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells. Nat Commun. 7:136082016. View Article : Google Scholar : PubMed/NCBI

7 

Wang Y, Gu J, Lin X, Yan W, Yang W and Wu G: lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway. Oncol Lett. 15:5865–5870. 2018.PubMed/NCBI

8 

Yuan S, Liu Q, Hu Z, Zhou Z, Wang G, Li C, Xie W, Meng G, Xiang Y, Wu N, et al: Long non-coding RNA MUC5B-AS1 promotes metastasis through mutually regulating MUC5B expression in lung adenocarcinoma. Cell Death Dis. 9:4502018. View Article : Google Scholar : PubMed/NCBI

9 

Zeng B, Lin Z, Ye H, Cheng D, Zhang G, Zhou J, Huang Z, Wang M, Cai C, Zeng J, et al: Upregulation of LncDQ is associated with poor prognosis and promotes tumor progression via epigenetic regulation of the EMT pathway in HCC. Cell Physiol Biochem. 46:1122–1133. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Chen X, Li D, Gao Y, Tang W, Iw L, Cao Y and Hao B: Long intergenic noncoding RNA 00152 promotes glioma cell proliferation and invasion by interacting with MiR-16. Cell Physiol Biochem. 46:1055–1064. 2018. View Article : Google Scholar : PubMed/NCBI

11 

Wang R, Dong HX, Zeng J, Pan J and Jin XY: LncRNA DGCR5 contributes to CSC-like properties via modulating miR-330-5p/CD44 in NSCLC. J Cell Physiol. 233:7447–7456. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Yong S, Yabin Y, Bing Z, Chuanrong Z, Dianhua G, Jianhuai Z, Weidong Y, Shuming W and Ling L: Reciprocal regulation of DGCR5 and miR-320a affects the cellular malignant phenotype and 5-FU response in pancreatic ductal adenocarcinoma. Oncotarget. 8:90868–90878. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Huang R, Wang X, Zhang W, Zhangyuan G, Jin K, Yu W, Xie Y, Xu X, Wang H and Sun B: Down-regulation of LncRNA DGCR5 correlates with poor prognosis in hepatocellular carcinoma. Cell Physiol Biochem. 40:707–715. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Ye B, Liu B, Yang L, Zhu X, Zhang D, Wu W, Zhu P, Wang Y, Wang S, Xia P, et al: LncKdm2b controls self-renewal of embryonic stem cells via activating expression of transcription factor Zbtb3. EMBO J. 37:e971742018. View Article : Google Scholar : PubMed/NCBI

16 

Zhu P, Wang Y, Huang G, Ye B, Liu B, Wu J, Du Y, He L and Fan Z: lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal. Nat Struct Mol Biol. 23:631–639. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Lan X, Zhang H, Wang Z, Dong W, Sun W, Shao L, Zhang T and Zhang D: Genome-wide analysis of long noncoding RNA expression profile in papillary thyroid carcinoma. Gene. 569:109–117. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Wang C, Yan G, Zhang Y, Jia X and Bu P: Long non-coding RNA MEG3 suppresses migration and invasion of thyroid carcinoma by targeting of Rac1. Neoplasma. 62:541–549. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Gou Q, Gao L, Nie X, Pu W, Zhu J, Wang Y, Liu X, Tan S, Zhou JK, Gong Y, et al: Long noncoding RNA AB074169 inhibits cell proliferation via modulation of KHSRP-mediated p21 expression in papillary thyroid carcinoma. Cancer Res. 78:4163–4174. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Ding S, Qu W, Jiao Y, Zhang J, Zhang C and Dang S: LncRNA SNHG12 promotes the proliferation and metastasis of papillary thyroid carcinoma cells through regulating wnt/β-catenin signaling pathway. Cancer Biomark. 22:217–226. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Wang X, Lu X, Geng Z, Yang G and Shi Y: LncRNA PTCSC3/miR-574-5p governs cell proliferation and migration of papillary thyroid carcinoma via Wnt/β-catenin signaling. J Cell Biochem. 118:4745–4752. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Li T, Yang XD, Ye CX, Shen ZL, Yang Y, Wang B, Guo P, Gao ZD, Ye YJ, Jiang KW and Wang S: Long noncoding RNA HIT000218960 promotes papillary thyroid cancer oncogenesis and tumor progression by upregulating the expression of high mobility group AT-hook 2 (HMGA2) gene. Cell Cycle. 16:224–231. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Sun W, Lan X, Wang Z, Dong W, He L, Zhang T and Zhang H: Overexpression of long non-coding RNA NR_036575.1 contributes to the proliferation and migration of papillary thyroid cancer. Med Oncol. 33:1022016. View Article : Google Scholar : PubMed/NCBI

25 

Chen EG, Zhang JS, Xu S, Zhu XJ and Hu HH: Long non-coding RNA DGCR5 is involved in the regulation of proliferation, migration and invasion of lung cancer by targeting miR-1180. Am J Cancer Res. 7:1463–1475. 2017.PubMed/NCBI

26 

Wang Y, Zeng X, Wang N, Zhao W, Zhang X, Teng S, Zhang Y and Lu Z: Long noncoding RNA DANCR, working as a competitive endogenous RNA, promotes ROCK1-mediated proliferation and metastasis via decoying of miR-335-5p and miR-1972 in osteosarcoma. Mol Cancer. 17:892018. View Article : Google Scholar : PubMed/NCBI

27 

Wang Z, Zhang H, Zhang P, Li J, Shan Z and Teng W: Upregulation of miR-2861 and miR-451 expression in papillary thyroid carcinoma with lymph node metastasis. Med Oncol. 30:5772013. View Article : Google Scholar : PubMed/NCBI

28 

Zhao M, Li L, Zhou J, Cui X, Tian Q, Jin Y and Zhu Y: MiR-2861 behaves as a biomarker of lung cancer stem cells and regulates the HDAC5-ERK system genes. Cell Reprogram. 20:99–106. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Xu J, Wan X, Chen X, Fang Y, Cheng X, Xie X and Lu W: miR-2861 acts as a tumor suppressor via targeting EGFR/AKT2/CCND1 pathway in cervical cancer induced by human papillomavirus virus 16 E6. Sci Rep. 6:289682016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen F, Yin S, Zhu J, Liu P, Yang C, Feng Z and Deng Z: lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861. Exp Ther Med 17: 895-900, 2019.
APA
Chen, F., Yin, S., Zhu, J., Liu, P., Yang, C., Feng, Z., & Deng, Z. (2019). lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861. Experimental and Therapeutic Medicine, 17, 895-900. https://doi.org/10.3892/etm.2018.7012
MLA
Chen, F., Yin, S., Zhu, J., Liu, P., Yang, C., Feng, Z., Deng, Z."lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861". Experimental and Therapeutic Medicine 17.1 (2019): 895-900.
Chicago
Chen, F., Yin, S., Zhu, J., Liu, P., Yang, C., Feng, Z., Deng, Z."lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861". Experimental and Therapeutic Medicine 17, no. 1 (2019): 895-900. https://doi.org/10.3892/etm.2018.7012
Copy and paste a formatted citation
x
Spandidos Publications style
Chen F, Yin S, Zhu J, Liu P, Yang C, Feng Z and Deng Z: lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861. Exp Ther Med 17: 895-900, 2019.
APA
Chen, F., Yin, S., Zhu, J., Liu, P., Yang, C., Feng, Z., & Deng, Z. (2019). lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861. Experimental and Therapeutic Medicine, 17, 895-900. https://doi.org/10.3892/etm.2018.7012
MLA
Chen, F., Yin, S., Zhu, J., Liu, P., Yang, C., Feng, Z., Deng, Z."lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861". Experimental and Therapeutic Medicine 17.1 (2019): 895-900.
Chicago
Chen, F., Yin, S., Zhu, J., Liu, P., Yang, C., Feng, Z., Deng, Z."lncRNA DGCR5 acts as a tumor suppressor in papillary thyroid carcinoma via sequestering miR‑2861". Experimental and Therapeutic Medicine 17, no. 1 (2019): 895-900. https://doi.org/10.3892/etm.2018.7012
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