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Effects of paraquat on IL‑6 and TNF‑α in macrophages

  • Authors:
    • Jie Huang
    • Ning Ning
    • Weiwei Zhang

  • View Affiliations

  • Published online on: December 14, 2018     https://doi.org/10.3892/etm.2018.7099
  • Pages: 1783-1789

  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effects of paraquat (PQ) on interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α) in macrophages were investigated. Different concentrations of PQ were added to mouse macrophage RAW264.7 for culture. According to different concentrations of PQ, mice were divided into micro concentration (0.01 mmol/l), low concentration (0.1 mmol/l), medium concentration (1 mmol/l), high concentration (10 mmol/l), and control groups without PQ. Trypan blue solution was used for detecting cell viability, a microplate reader for detecting the fluorescence intensity of reactive oxygen species (ROS), ELISA for detecting the expression levels of IL‑6 and TNF‑α. The medium concentration and the high concentration groups had significantly lower cell viability than the other three groups (P<0.050). The high concentration group had significantly lower cell viability than the medium concentration group (P<0.050). At 1, 4 and 8 h, respectively, the medium and the high concentration groups had significantly higher ROS fluorescence intensity than the other three groups (P<0.050). The high concentration group had significantly higher ROS fluorescence intensity than the medium concentration group (P<0.050). There were significant differences in the expression levels of IL‑6 and TNF‑α at the 1st, 4th and 8th hour among the five groups (P<0.050). In the micro, the low, the medium and high concentration groups, the expression levels of IL‑6 and TNF‑α were the lowest at 1 h and the highest at 8 h, which were higher at 4 h than those at 1 h (P<0.050). PQ at a concentration of 1 mmol/l can produce toxicity to macrophages, and greatly increase the ROS fluorescence intensity, the expression levels of IL‑6 and TNF‑α. PQ poisoning is expected to be treated though IL‑6 and TNF‑α in the future.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
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Spandidos Publications style
Huang J, Ning N and Zhang W: Effects of paraquat on IL‑6 and TNF‑α in macrophages. Exp Ther Med 17: 1783-1789, 2019.
APA
Huang, J., Ning, N., & Zhang, W. (2019). Effects of paraquat on IL‑6 and TNF‑α in macrophages. Experimental and Therapeutic Medicine, 17, 1783-1789. https://doi.org/10.3892/etm.2018.7099
MLA
Huang, J., Ning, N., Zhang, W."Effects of paraquat on IL‑6 and TNF‑α in macrophages". Experimental and Therapeutic Medicine 17.3 (2019): 1783-1789.
Chicago
Huang, J., Ning, N., Zhang, W."Effects of paraquat on IL‑6 and TNF‑α in macrophages". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1783-1789. https://doi.org/10.3892/etm.2018.7099