Open Access

Protective effect of Xin‑Ji‑Er‑Kang on cardiovascular remodeling in high salt‑induced hypertensive mice

  • Authors:
    • Guangyao Huang
    • Pan Cheng
    • Ling Ding
    • Li Wang
    • Juan Hu
    • Yongxue Zhang
    • Guowei Cai
    • Meiling Chen
    • Aizong Shen
    • Shan Gao
  • View Affiliations

  • Published online on: December 17, 2018     https://doi.org/10.3892/etm.2018.7105
  • Pages: 1551-1562
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effects of Xin‑Ji‑Er‑Kang (XJEK) on high salt‑induced hypertensive mice. Mice with high‑salt diet‑induced hypertension were divided into four groups: Control (standard diet alone for 8 weeks), model (diet containing 8% NaCl for 8 weeks and intragastric administration of distilled water for the last 4 weeks), XJEK + high‑salt‑treated (diet containing 8% NaCl for 8 weeks and intragastric administration of XJEK for the last 4 weeks) and irbesartan + high‑salt‑treated (diet containing 8% NaCl for 8 weeks with intragastric administration of irbesartan for the last 4 weeks). The hemodynamic index and cardiac pathological changes in the hypertensive mice were then examined. An aortic ring apparatus was used to detect acetylcholine‑dependent endothelium relaxation function. Colorimetric analysis was applied to determine serum nitric oxide (NO), superoxide dismutase activity and malondialdehyde content; ELISA was employed to measure brain natriuretic peptide, serum angiotensin II (Ang II), endothelin‑1 content and aldosterone; and immunohistochemistry was used to detect the expression of endothelial nitric oxide synthase (eNOS), interleukin (IL)‑1β, IL‑10 and tumor necrosis factor (TNF)‑α in cardiac tissues. XJEK improved the heart systolic and diastolic function, ameliorated hemodynamic parameters and cardiovascular remodeling indices, blunted the cardiac pathological changes and improved endothelial dysfunction (ED) via boosting eNOS activity, promoting NO bioavailability and decreasing serum Ang II content. Furthermore, treatment with XJEK inhibited the increase of IL‑1β and TNF‑α expression and the decrease of IL‑10 expression in cardiac tissues, and ameliorated oxidative stress status. Therefore, XJEK exerted protective effects against high salt‑induced hypertension and cardiovascular remodeling in mice via improving ED, restoring pro‑ and anti‑inflammatory factor balance and decreasing oxidative stress.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Huang G, Cheng P, Ding L, Wang L, Hu J, Zhang Y, Cai G, Chen M, Shen A, Gao S, Gao S, et al: Protective effect of Xin‑Ji‑Er‑Kang on cardiovascular remodeling in high salt‑induced hypertensive mice. Exp Ther Med 17: 1551-1562, 2019
APA
Huang, G., Cheng, P., Ding, L., Wang, L., Hu, J., Zhang, Y. ... Gao, S. (2019). Protective effect of Xin‑Ji‑Er‑Kang on cardiovascular remodeling in high salt‑induced hypertensive mice. Experimental and Therapeutic Medicine, 17, 1551-1562. https://doi.org/10.3892/etm.2018.7105
MLA
Huang, G., Cheng, P., Ding, L., Wang, L., Hu, J., Zhang, Y., Cai, G., Chen, M., Shen, A., Gao, S."Protective effect of Xin‑Ji‑Er‑Kang on cardiovascular remodeling in high salt‑induced hypertensive mice". Experimental and Therapeutic Medicine 17.3 (2019): 1551-1562.
Chicago
Huang, G., Cheng, P., Ding, L., Wang, L., Hu, J., Zhang, Y., Cai, G., Chen, M., Shen, A., Gao, S."Protective effect of Xin‑Ji‑Er‑Kang on cardiovascular remodeling in high salt‑induced hypertensive mice". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1551-1562. https://doi.org/10.3892/etm.2018.7105