Open Access

Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats

  • Authors:
    • Jiahui Zheng
    • Shufeng Zhang
    • Tingting Wang
  • View Affiliations

  • Published online on: December 21, 2018     https://doi.org/10.3892/etm.2018.7123
  • Pages: 1587-1592
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expression changes of Notch-1 and nuclear factor-κB (NF-κB) in cardiomyocytes from coronary heart disease (CHD) rats were investigated. The CHD model established by high-fat diet in 48 clean SD rats was set as the observation group, and another 48 healthy rats routinely fed were the control group. Rats were sacrificed on the 1st, 3rd, 7th, and 14th days after successful modeling. The rat myocardial cells were harvested to examine the changes of Notch-1 and NF-κB using immunoblot (western blot analysis) and TUNEL assay. Cardiomyocyte apoptotic rate, by Pearson's correlation test was used to analyze the correlation between Notch-1 and NF-κB. With the prolongation of the course of CHD in rats, the expression levels of Notch-1 and NF-κB proteins gradually increased, and the expression in the observation group was significantly higher than that in the control group (P<0.01). On the seventh day, the expression levels of Notch-1 and NF-κB protein in the rats in the observation group showed significant difference from those on day 1 and day 3 (P<0.05), and they were significantly different on the 7th and 14th days (P<0.05). There was no correlation between the expression level of the two proteins with the age and sex of the rats. Pearson's correlation analysis showed that Notch-1 was positively correlated with NF-κB protein (r=0.745, P=0.005). The results of myocardial apoptosis test showed that with the prolongation of the CHD course in the rats of the observation group, the cardiomyocyte apoptosis rate did not differ from the control group. The expression of Notch-1 and NF-κB protein is increased in cardiomyocytes of CHD rats. Notch-1 and NF-κB participate in the occurrence and development of CHD.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zheng J, Zhang S and Wang T: Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats. Exp Ther Med 17: 1587-1592, 2019
APA
Zheng, J., Zhang, S., & Wang, T. (2019). Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats. Experimental and Therapeutic Medicine, 17, 1587-1592. https://doi.org/10.3892/etm.2018.7123
MLA
Zheng, J., Zhang, S., Wang, T."Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats". Experimental and Therapeutic Medicine 17.3 (2019): 1587-1592.
Chicago
Zheng, J., Zhang, S., Wang, T."Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1587-1592. https://doi.org/10.3892/etm.2018.7123