MicroRNA‑761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D

Ren,Yupeng; Shi,Gang; Jiang,Peng; Meng,Qingkai

doi:10.3892/etm.2018.7126

March-2019 Volume 17 Issue 3

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March-2019 Volume 17 Issue 3

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Article

MicroRNA‑761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D

Authors:
- Yupeng Ren
- Gang Shi
- Peng Jiang
- Qingkai Meng
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Affiliations: Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China
Pages: 1841-1846
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Published online on: December 21, 2018

https://doi.org/10.3892/etm.2018.7126
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Abstract

The purpose of the present study was to investigate the biological role of microRNA‑761 (miR‑761) in colorectal cancer (CRC) and the underlying mechanisms by which miR‑761 regulates CRC cell proliferation and migration. Quantitative polymerase chain reaction was performed to measure miR‑761 expression in CRC tumor tissues and cell lines. It was demonstrated that miR‑761 expression was dramatically reduced in CRC tumor tissues and cell lines compared with in normal tissues and cell lines. Overexpression of miR‑761 significantly decreased CRC cell growth and migration. Using bioinformatics analysis and luciferase reporter assays, Rab3D was identified as a novel target of miR‑761. In addition, it was demonstrated that Rab3D expression was negatively correlated with miR‑761. Furthermore, overexpression of Rab3D could reverse the inhibitory effects of miR‑761 on cell proliferation and migration. Collectively, the present study demonstrated that miR‑761 overexpression could inhibit the proliferation and migration of CRC cell lines, partly at least, via directly targeting Rab3D.

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1	Goss PE, Strasser-Weippl K, Lee-Bychkovsky BL, Fan L, Li J, Chavarri-Guerra Y, Liedke PE, Pramesh CS, Badovinac-Crnjevic T, Sheikine Y, et al: Challenges to effective cancer control in China, India, and Russia. Lancet Oncol. 15:489–538. 2014. View Article : Google Scholar : PubMed/NCBI
2	Varghese C and Shin HR: Strengthening cancer control in China. Lancet Oncol. 15:484–485. 2014. View Article : Google Scholar : PubMed/NCBI
3	Chakradhar S: Colorectal cancer: 5 big questions. Nature. 521:S162015. View Article : Google Scholar : PubMed/NCBI
4	Brody H: Colorectal cancer. Nature. 521:S12015. View Article : Google Scholar : PubMed/NCBI
5	Ohtsuka M, Ling H, Doki Y, Mori M and Calin GA: MicroRNA processing and human cancer. J Clin Med. 4:1651–1667. 2015. View Article : Google Scholar : PubMed/NCBI
6	Melo SA and Esteller M: Dysregulation of microRNAs in cancer: Playing with fire. FEBS Lett. 585:2087–2099. 2011. View Article : Google Scholar : PubMed/NCBI
7	Moretti F, Thermann R and Hentze MW: Mechanism of translational regulation by miR-2 from sites in the 5′ untranslated region or the open reading frame. RNA. 16:2493–2502. 2010. View Article : Google Scholar : PubMed/NCBI
8	Visone R and Croce CM: MiRNAs and cancer. Am J Pathol. 174:1131–1138. 2009. View Article : Google Scholar : PubMed/NCBI
9	Di Leva G and Croce CM: miRNA profiling of cancer. Curr Opin Genet Dev. 23:3–11. 2013. View Article : Google Scholar : PubMed/NCBI
10	Zhou X, Zhang L, Zheng B, Yan Y, Zhang Y, Xie H, Zhou L, Zheng S and Wang W: MicroRNA-761 is upregulated in hepatocellular carcinoma and regulates tumorigenesis by targeting Mitofusin-2. Cancer Sci. 107:424–432. 2016. View Article : Google Scholar : PubMed/NCBI
11	Yan A, Yang C, Chen Z, Li C and Cai L: MiR-761 promotes progression and metastasis of non-small cell lung cancer by targeting ING4 and TIMP2. Cell Physiol Biochem. 37:55–66. 2015. View Article : Google Scholar : PubMed/NCBI
12	Shi C and Zhang Z: miR-761 inhibits tumor progression by targeting MSI1 in ovarian carcinoma. Tumour Biol. 37:5437–5443. 2016. View Article : Google Scholar : PubMed/NCBI
13	Cao S, Lin L, Xia X and Wu H: MicroRNA-761 promotes the sensitivity of colorectal cancer cells to 5-Fluorouracil through targeting FOXM1. Oncotarget. 9:321–331. 2017.PubMed/NCBI
14	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
15	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI
16	Jiashi W, Chuang Q, Zhenjun Z, Guangbin W, Bin L and Ming H: MicroRNA-506-3p inhibits osteosarcoma cell proliferation and metastasis by suppressing RAB3D expression. Aging (Albany NY). 10:1294–1305. 2018. View Article : Google Scholar : PubMed/NCBI
17	Zhang J, Kong RR and Sun LZ: Silencing of Rab3D suppresses the proliferation and invasion of esophageal squamous cell carcinoma cells. Biomed Pharmacother. 91:402–407. 2017. View Article : Google Scholar : PubMed/NCBI
18	Luo Y, Ye GY, Qin SL, Mu YF, Zhang L, Qi Y, Qiu YE, Yu MH and Zhong M: High expression of Rab3D predicts poor prognosis and associates with tumor progression in colorectal cancer. Int J Biochem Cell Biol. 75:53–62. 2016. View Article : Google Scholar : PubMed/NCBI
19	Yang J, Liu W, Lu X, Fu Y, Li L and Luo Y: High expression of small GTPase Rab3D promotes cancer progression and metastasis. Oncotarget. 6:11125–11138. 2015.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA‑761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D

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