MicroRNA‑761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D

Ren,Yupeng; Shi,Gang; Jiang,Peng; Meng,Qingkai

doi:10.3892/etm.2018.7126

MicroRNA‑761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D

Authors:
- Yupeng Ren
- Gang Shi
- Peng Jiang
- Qingkai Meng
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Affiliations: Department of Colorectal Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China
Published online on: December 21, 2018 https://doi.org/10.3892/etm.2018.7126
Pages: 1841-1846

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Abstract

The purpose of the present study was to investigate the biological role of microRNA‑761 (miR‑761) in colorectal cancer (CRC) and the underlying mechanisms by which miR‑761 regulates CRC cell proliferation and migration. Quantitative polymerase chain reaction was performed to measure miR‑761 expression in CRC tumor tissues and cell lines. It was demonstrated that miR‑761 expression was dramatically reduced in CRC tumor tissues and cell lines compared with in normal tissues and cell lines. Overexpression of miR‑761 significantly decreased CRC cell growth and migration. Using bioinformatics analysis and luciferase reporter assays, Rab3D was identified as a novel target of miR‑761. In addition, it was demonstrated that Rab3D expression was negatively correlated with miR‑761. Furthermore, overexpression of Rab3D could reverse the inhibitory effects of miR‑761 on cell proliferation and migration. Collectively, the present study demonstrated that miR‑761 overexpression could inhibit the proliferation and migration of CRC cell lines, partly at least, via directly targeting Rab3D.

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