Open Access

Epigallocatechin gallate inhibits cell growth and regulates miRNA expression in cervical carcinoma cell lines infected with different high‑risk human papillomavirus subtypes

  • Authors:
    • Yu Zhu
    • Yongfang Huang
    • Mingmin Liu
    • Qi Yan
    • Wanhong Zhao
    • Ping Yang
    • Qin Gao
    • Juanjuan Wei
    • Wenxia Zhao
    • Lishan Ma
  • View Affiliations

  • Published online on: December 24, 2018     https://doi.org/10.3892/etm.2018.7131
  • Pages: 1742-1748
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the inhibitory effects of the polyphenol epigallocatechin‑3‑gallate (EGCG) on the growth of cervical carcinoma cell lines infected with different high‑risk human papillomavirus (HPV) subtypes, as well as the associated regulation of microRNA (miR) expression. Cell proliferation was measured using an MTT assay. The effects of 7 different concentrations of EGCG (100, 80, 60, 40, 20, 10 and 0 µg/ml) on HeLa cell proliferation were assessed. HeLa cell growth was significantly inhibited by EGCG in a dose‑ and time‑dependent manner (P<0.05), and the IC50 was 90.74 and 72.74 µg/ml at 24 and 48 h, respectively. The expression of miR‑210, miR‑29a, miR‑203 and miR‑125b in HeLa (HPV16/18+), SiHa (HPV16+), CaSki (HPV16+) and C33A (HPV‑) cell lines was measured using quantitative polymerase chain reaction analysis. In CA33 cells, miR‑203 (all P<0.001) and miR‑125b (P<0.01 and <0.0001) were significantly downregulated by EGCG, and miR‑210 was significantly upregulated with 40 and 60 µg/ml EGCG (P<0.0001). miR‑125b was significantly downregulated (P<0.001 and <0.0001), and miR‑210 and miR‑29 were significantly upregulated by ≤80 µg/ml EGCG in HeLa cells (all P<0.0001). In CaSki cells, miR‑210, miR‑29a (all P<0.001) and miR‑125b (P<0.01‑0.0001) were significantly upregulated by EGCG. In SiHa cells, miR‑125b (both P<0.001) and miR‑203 (P<0.01 and <0.0001) were significantly upregulated by EGCG. In conclusion, the results of the present study suggest that EGCG suppresses cervical carcinoma cell growth, possibly via regulating the expression of miRs, suggesting their potential as therapeutic targets for the control and prevention of cervical cancer. Additionally, EGCG may be considered a novel anti‑cervical cancer drug in the future.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
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Spandidos Publications style
Zhu Y, Huang Y, Liu M, Yan Q, Zhao W, Yang P, Gao Q, Wei J, Zhao W, Ma L, Ma L, et al: Epigallocatechin gallate inhibits cell growth and regulates miRNA expression in cervical carcinoma cell lines infected with different high‑risk human papillomavirus subtypes. Exp Ther Med 17: 1742-1748, 2019.
APA
Zhu, Y., Huang, Y., Liu, M., Yan, Q., Zhao, W., Yang, P. ... Ma, L. (2019). Epigallocatechin gallate inhibits cell growth and regulates miRNA expression in cervical carcinoma cell lines infected with different high‑risk human papillomavirus subtypes. Experimental and Therapeutic Medicine, 17, 1742-1748. https://doi.org/10.3892/etm.2018.7131
MLA
Zhu, Y., Huang, Y., Liu, M., Yan, Q., Zhao, W., Yang, P., Gao, Q., Wei, J., Zhao, W., Ma, L."Epigallocatechin gallate inhibits cell growth and regulates miRNA expression in cervical carcinoma cell lines infected with different high‑risk human papillomavirus subtypes". Experimental and Therapeutic Medicine 17.3 (2019): 1742-1748.
Chicago
Zhu, Y., Huang, Y., Liu, M., Yan, Q., Zhao, W., Yang, P., Gao, Q., Wei, J., Zhao, W., Ma, L."Epigallocatechin gallate inhibits cell growth and regulates miRNA expression in cervical carcinoma cell lines infected with different high‑risk human papillomavirus subtypes". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1742-1748. https://doi.org/10.3892/etm.2018.7131