Discrimination of Burkholderia gladioli pv.alliicola and B. cepacia complex using the gyrB gene of B. gladioli pv. alliicola

Zhang,Lun; Gao,Wenna; Yin,Youping; Wang,Zhongkang

doi:10.3892/etm.2018.7137

Discrimination of Burkholderia gladioli pv.alliicola and B. cepacia complex using the gyrB gene of B. gladioli pv. alliicola

Authors:
- Lun Zhang
- Wenna Gao
- Youping Yin
- Zhongkang Wang
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Affiliations: School of Life Sciences, Chongqing University, Shapingba, Chongqing 400030, P.R. China, Beijing Entry‑Exit Inspection and Quarantine Bureau, Beijing 100026, P.R. China
Published online on: December 28, 2018 https://doi.org/10.3892/etm.2018.7137
Pages: 1870-1876

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Abstract

The aim of the present study was to investigate the efficiency of the gyrB gene derived from Burkholderia gladioli pv.Alliicola (Bga) on the identification of Bga from the B. cepacia complex (Bcc) based on the COnsensus‑DEgenerate Hybrid Oligonucleotide Primer (CODEHOP) strategy. A set of primers used for the specific amplification of the gyrB gene in Bga were designed according to the CODEHOP principle. A total of 1,644 bp of the gyrB gene sequence of Bga were acquired by CODEHOP amplification. The sequence was blasted in GenBank and it revealed an average of 86% similarity with the gyrB gene of nine genomovars of Bcc. A phylogenetic tree was constructed using the gyrB gene sequences. The microarray method was adopted to discriminate Bga from Bcc based on the specific probes designed upon the gyrB gene, and five genomovars of Bcc demonstrated a good discrimination from Bga on the microarray chip. CODEHOP strategy succeeded in amplification of the gyrB gene of Bga, which made it possible for the identification of Bga from five genomovars of Bcc.

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