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Article

The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family

  • Authors:
    • Lin Lin
    • Peng Cui
    • Zhipeng Qiu
    • Min Wang
    • Yingchao Yu
    • Jing Wang
    • Qian Sun
    • Hairong Zhao
  • View Affiliations / Copyright

    Affiliations: Health Examination Department, Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Multidisciplinary Consultation Center, Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Emergency Department, Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Joint Surgery, Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of General Medicine, Affiliated Qingdao Hiser Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China
  • Pages: 1855-1862
    |
    Published online on: December 28, 2018
       https://doi.org/10.3892/etm.2018.7143
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Abstract

Hypertension is a very common cardiovascular disorder, however, the molecular mechanism underlying this disease remains poorly understood. Recently, an increasing number of studies have demonstrated that mitochondrial (mt)DNA mutations serve important roles in the pathogenesis of hypertension. The current study reported the clinical and molecular characterization of a Chinese family with maternally inherited hypertension (the penetrance of hypertension was 71.4%). In addition, the entire mitochondrial transfer (mt‑t)RNA genomes was amplified using a polymerase chain reaction (PCR) and identified through direct Sanger sequencing. Additionally, the mtDNA copy number in matrilineal relatives in this family was evaluated using quantitative PCR. The sequence analysis of the 22 mt‑tRNA genes led to the identification of tRNAAla 5587T>C (thymine to cytosine) and tRNALeu(CUN) 12280A>G (adenine to guanine) mutations. Notably, the heteroplasmic 5587T>C mutation was located at the 3' end of tRNAAla (position 73), which is highly conserved from bacteria to human mitochondria. In addition, the 12280A>G mutation was revealed to occurs at the dihydrouridine loop of tRNALeu(CUN) (position 15) and may decrease the steady‑state level of mt‑tRNA. As a result, 5587T>C and 12280A>G mutations may lead to the failure of tRNAs metabolism and subsequently cause mitochondrial protein synthesis defects. Molecular analysis revealed that patients carrying the 5587T>C and 12280A>G mutations had a lower copy number of mtDNA compared with a control with hypertension, but without the mutations, suggesting that these mutations may cause mitochondrial dysfunctions that are responsible for hypertension. Therefore, mt‑tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be involved in the pathogenesis of hypertension in this family.
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Copy and paste a formatted citation
Spandidos Publications style
Lin L, Cui P, Qiu Z, Wang M, Yu Y, Wang J, Sun Q and Zhao H: The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family. Exp Ther Med 17: 1855-1862, 2019.
APA
Lin, L., Cui, P., Qiu, Z., Wang, M., Yu, Y., Wang, J. ... Zhao, H. (2019). The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family. Experimental and Therapeutic Medicine, 17, 1855-1862. https://doi.org/10.3892/etm.2018.7143
MLA
Lin, L., Cui, P., Qiu, Z., Wang, M., Yu, Y., Wang, J., Sun, Q., Zhao, H."The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family". Experimental and Therapeutic Medicine 17.3 (2019): 1855-1862.
Chicago
Lin, L., Cui, P., Qiu, Z., Wang, M., Yu, Y., Wang, J., Sun, Q., Zhao, H."The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1855-1862. https://doi.org/10.3892/etm.2018.7143
Copy and paste a formatted citation
x
Spandidos Publications style
Lin L, Cui P, Qiu Z, Wang M, Yu Y, Wang J, Sun Q and Zhao H: The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family. Exp Ther Med 17: 1855-1862, 2019.
APA
Lin, L., Cui, P., Qiu, Z., Wang, M., Yu, Y., Wang, J. ... Zhao, H. (2019). The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family. Experimental and Therapeutic Medicine, 17, 1855-1862. https://doi.org/10.3892/etm.2018.7143
MLA
Lin, L., Cui, P., Qiu, Z., Wang, M., Yu, Y., Wang, J., Sun, Q., Zhao, H."The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family". Experimental and Therapeutic Medicine 17.3 (2019): 1855-1862.
Chicago
Lin, L., Cui, P., Qiu, Z., Wang, M., Yu, Y., Wang, J., Sun, Q., Zhao, H."The mitochondrial tRNAAla 5587T>C and tRNALeu(CUN) 12280A>G mutations may be associated with hypertension in a Chinese family". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1855-1862. https://doi.org/10.3892/etm.2018.7143
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