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3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression

  • Authors:
    • Jun Guo
    • Chunting Zhao
    • Ruyong Yao
    • Aihua Sui
    • Lingjie Sun
    • Xiaodan Liu
    • Shaoling Wu
    • Zhan Su
    • Tianlan Li
    • Shanshan Liu
    • Yan Gao
    • Jiaxiu Liu
    • Xianqi Feng
    • Wei Wang
    • Hongguo Zhao
    • Zhongguang Cui
    • Guanglun Li
    • Fanjun Meng
  • View Affiliations / Copyright

    Affiliations: College of Medicine, Qingdao University, Qingdao, Shandong 266071, P.R. China, Department of Hematology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266555, P.R. China, Central Laboratory, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266035, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1593-1600
    |
    Published online on: January 4, 2019
       https://doi.org/10.3892/etm.2019.7153
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Abstract

Three dimensional (3D) culture has gradually become a research hotspot in the field of drug screening, stem cell research, and tissue engineering due to its more physiological-like morphology and function. In this study, we compared the differences of cell proliferation, population, protein expression and chemoresistance profiles between two dimensional (2D) and 3D culture of acute lymphoblastic leukemia (ALL) Jurkat cell line. Polycaprolactone (PCL) is used for 3D culture owing to its biochemical properties and compatibility. Culturing of ALL Jurkat cell line in collagen type Ⅰ coated polycaprolactone scaffold for 168 h increased cell proliferation, attachment, as well as the drug resistance to cytarabine (Ara-C) and daunorubicin (DNR) without changing the original CD2+CD3+CD4+dimCD8-CD34-CD45+dim phenotype, compared to uncoated PCL scaffold and tissue culture plate systems. Molecularly, increased chemoresistance is associated with the upregulation of discoidin domain receptor 1 (DDR1) and transcription factor STAT3. Inhibition of DDR1 activity by DDR1-specific inhibitor DDR-IN-1 accelerated cell death in the presence of Ara-C, DNR or their combination. These results demonstrated that 3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression. Importantly, the cell adhesion-mediated drug resistance induced by DDR1 in the scaffold was similar to the clinical situation, indicating the 3D culture of cancer cells recapitulate the in vivo tumor environment and this platform can be used as a promising pre-clinic drug-screen system.
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1 

Nair MS, Mony U, Menon D, Koyakutty M, Sidharthan N, Pavithran K, Nair SV and Menon KN: Development and molecular characterization of polymeric micro-nanofibrous scaffold of a defined 3-D niche for in vitro chemosensitivity analysis against acute myeloid leukemia cells. Int J Nanomedicine. 10:3603–3622. 2015.PubMed/NCBI

2 

Funayama K, Murai F, Shimane M, Nomura H and Asano S: Adhesion-induced drug resistance in leukemia stem cells. Pharmacology. 86:79–84. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Wojtkowiak JW, Verduzco D, Schramm KJ and Gillies RJ: Drug resistance and cellular adaptation to tumor acidic pH microenvironment. Mol Pharm. 8:2032–2038. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Wang JY, Yu P, Chen S, Xing H, Chen Y, Wang M, Tang K, Tian Z, Rao Q and Wang J: Activation of Rac1 GTPase promotes leukemia cell chemotherapy resistance, quiescence and niche interaction. Mol Oncol. 7:907–916. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Dumbleton J, Agarwal P, Huang H, Hogrebe N, Han R, Gooch KJ and He X: The effect of RGD peptide on 2D and miniaturized 3D culture of HEPM cells, MSCs, and ADSCs with alginate hydrogel. Cell Mol Bioeng. 9:277–288. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Duval K, Grover H, Han LH, Mou Y, Pegoraro AF, Fredberg J and Chen Z: Modeling physiological events in 2D vs. 3D cell culture. Physiology (Bethesda). 32:266–277. 2017.PubMed/NCBI

7 

Nugraha B, Mohr MA, Ponti A, Emmert MY, Weibel F, Hoerstrup SP, Moll S, Certa U, Prunotto M and Pantazis P: Monitoring and manipulating cellular crosstalk during kidney fibrosis inside a 3D in vitro co-culture. Sci Rep. 7:144902017. View Article : Google Scholar : PubMed/NCBI

8 

Hou J, Hong Z, Feng F, Chai Y, Zhang Y, Jiang Q, Hu Y, Wu S, Wu Y, Gao X, et al: A novel chemotherapeutic sensitivity-testing system based on collagen gel droplet embedded 3D-culture methods for hepatocellular carcinoma. BMC Cancer. 17:7292017. View Article : Google Scholar : PubMed/NCBI

9 

Burton TP, Corcoran A and Callanan A: The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells. Biomed Mater. 13:0150062017. View Article : Google Scholar : PubMed/NCBI

10 

Caicedo-Carvajal CE, Liu Q, Remache Y, Goy A and Suh KS: Cancer tissue engineering: A novel 3D polystyrene scaffold for in vitro isolation and amplification of lymphoma cancer cells from heterogeneous cell mixtures. J Tissue Eng. 2011:3623262011.PubMed/NCBI

11 

Hur H, Ham IH, Lee D, Jin H, Aguilera KY, Oh HJ, Han SU, Kwon JE, Kim YB, Ding K, et al: Discoidin domain receptor 1 activity drives an aggressive phenotype in gastric carcinoma. BMC Cancer. 17:872017. View Article : Google Scholar : PubMed/NCBI

12 

Moreau V and Saltel F: Type I collagen fibrils and discoidin domain receptor 1 set invadosomes straight. Mol Cell Oncol. 2:e10049632015. View Article : Google Scholar : PubMed/NCBI

13 

Stępnik M, Spryszyńska S, Gorzkiewicz A and Ferlińska M: Cytotoxicity of anticancer drugs and PJ-34 (poly(ADP-ribose)polymerase-1 (PARP-1) inhibitor) on HL-60 and Jurkat cells. Adv Clin Exp Med. 26:379–385. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Blanco TM, Mantalaris A, Bismarck A and Panoskaltsis N: The development of a three-dimensional scaffold for ex vivo biomimicry of human acute myeloid leukaemia. Biomaterials. 31:2243–2251. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Bao M, Xie J, Piruska A and Huck WTS: 3D microniches reveal the importance of cell size and shape. Nat Commun. 8:19622017. View Article : Google Scholar : PubMed/NCBI

16 

Bell CC, Dankers ACA, Lauschke VM, Sison-Young R, Jenkins R, Rowe C, Goldring CE, Park K, Regan SL, Walker T, et al: Comparison of hepatic 2D sandwich cultures and 3D spheroids for long-term toxicity applications: A multi-center study. Toxicol Sci. 162:655–666. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Xue R, Qian Y, Li L, Yao G, Yang L and Sun Y: Polycaprolactone nanofiber scaffold enhances the osteogenic differentiation potency of various human tissue-derived mesenchymal stem cells. Stem Cell Res Ther. 8:1482017. View Article : Google Scholar : PubMed/NCBI

18 

Yildirim ED, Besunder R, Pappas D, Allen F, Güçeri S and Sun W: Accelerated differentiation of osteoblast cells on polycaprolactone scaffolds driven by a combined effect of protein coating and plasma modification. Biofabrication. 2:0141092010. View Article : Google Scholar : PubMed/NCBI

19 

Das S, Ongusaha PP, Yang YS, Park JM, Aaronson SA and Lee SW: Discoidin domain receptor 1 receptor tyrosine kinase induces cyclooxygenase-2 and promotes chemoresistance through nuclear factor-kappaB pathway activation. Cancer Res. 66:8123–8130. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Canning P, Tan L, Chu K, Lee SW, Gray NS and Bullock AN: Structural mechanisms determining inhibition of the collagen receptor DDR1 by selective and multi-targeted type II kinase inhibitors. J Mol Biol. 426:2457–2470. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Kim HG, Tan L, Weisberg EL, Liu F, Canning P, Choi HG, Ezell SA, Wu H, Zhao Z, Wang J, et al: Discovery of a potent and selective DDR1 receptor tyrosine kinase inhibitor. ACS Chem Biol. 8:2145–2150. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Fang Y, Wang B, Zhao Y, Xiao Z, Li J, Cui Y, Han S, Wei J, Chen B, Han J, et al: Collagen scaffold microenvironments modulate cell lineage commitment for differentiation of bone marrow cells into regulatory dendritic cells. Sci Rep. 7:420492017. View Article : Google Scholar : PubMed/NCBI

23 

Lin CH, Chiang MC and Chen YJ: STAT3 mediates resistance to anoikis and promotes invasiveness of nasopharyngeal cancer cells. Int J Mol Med. 40:1549–1556. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Yang JC, Zhang Y, He SJ, Li MM, Cai XL, Wang H, Xu LM and Cao J: TM4SF1 promotes metastasis of pancreatic cancer via regulating the expression of DDR1. Sci Rep. 7:458952017. View Article : Google Scholar : PubMed/NCBI

25 

Gao H, Chakraborty G, Zhang Z, Akalay I, Gadiya M, Gao Y, Sinha S, Hu J, Jiang C, Akram M, et al: Multi-organ site metastatic reactivation mediated by non-canonical discoidin domain receptor 1 signaling. Cell. 166:47–62. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Wang Z, Sun X, Bao Y, Mo J, Du H, Hu J and Zhang X: E2F1 silencing inhibits migration and invasion of osteosarcoma cells via regulating DDR1 expression. Int J Oncol. 51:1639–1650. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Yang C and Zhang X: Incidence survey of leukemia in China. Chin Med Sci J. 6:65–70. 1991.PubMed/NCBI

28 

Broxterman HJ and Georgopapadakou NH: Anticancer therapeutics: ‘Addictive’ targets, multi-targeted drugs, new drug combinations. Drug Resist Updat. 8:183–197. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Da Costa EM, McInnes G, Beaudry A and Raynal NJ: DNA methylation-targeted drugs. Cancer J. 23:270–276. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Jamison C, Nelson D, Eren M, Gauchan D, Ramaekers R, Norvell M and Copur MS: What is the optimal dose and schedule for dasatinib in chronic myeloid leukemia: Two case reports and review of the literature. Oncol Res. 23:1–5. 2015. View Article : Google Scholar

31 

Wang JZ, Zhu YX, Ma HC, Chen SN, Chao JY, Ruan WD, Wang D, Du FG and Meng YZ: Developing multi-cellular tumor spheroid model (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. Mater Sci Eng C. 62:215–225. 2016. View Article : Google Scholar

32 

Poulos MG, Gars EJ, Gutkin MC, Kloss CC, Ginsberg M, Scandura JM, Rafii S and Butler JM: Activation of the vascular niche supports leukemic progression and resistance to chemotherapy. Exp Hematol. 42:976–986.e3. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Prieto-Vila M, Takahashi RU, Usuba W, Kohama I and Ochiya T: Drug resistance driven by cancer stem cells and their niche. Int J Mol Sci. 18:25742017. View Article : Google Scholar

34 

Feng Q, Chai C, Jiang XS, Leong KW and Mao HQ: Expansion of engrafting human hematopoietic stem/progenitor cells in three-dimensional scaffolds with surface-immobilized fibronectin. J Biomed Mater Res A. 78:781–791. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Deng Y, Zhao F, Hui L, Li X, Zhang D, Lin W, Chen Z and Ning Y: Suppressing miR-199a-3p by promoter methylation contributes to tumor aggressiveness and cisplatin resistance of ovarian cancer through promoting DDR1 expression. J Ovarian Res. 10:502017. View Article : Google Scholar : PubMed/NCBI

36 

Carbone A and Gloghini A: Activated DDR1 increases RS cell survival. Blood. 122:4152–4154. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Kim MK, Kim Y, Choo H and Chong Y: Quercetin-glutamic acid conjugate with a non-hydrolysable linker; a novel scaffold for multidrug resistance reversal agents through inhibition of P-glycoprotein. Bioorg Med Chem. 25:1219–1226. 2017. View Article : Google Scholar : PubMed/NCBI

38 

Yang Z and Zhao X: A 3D model of ovarian cancer cell lines on peptide nanofiber scaffold to explore the cell-scaffold interaction and chemotherapeutic resistance of anticancer drugs. Int J Nanomedicine. 6:303–310. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guo J, Zhao C, Yao R, Sui A, Sun L, Liu X, Wu S, Su Z, Li T, Liu S, Liu S, et al: 3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression. Exp Ther Med 17: 1593-1600, 2019.
APA
Guo, J., Zhao, C., Yao, R., Sui, A., Sun, L., Liu, X. ... Meng, F. (2019). 3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression. Experimental and Therapeutic Medicine, 17, 1593-1600. https://doi.org/10.3892/etm.2019.7153
MLA
Guo, J., Zhao, C., Yao, R., Sui, A., Sun, L., Liu, X., Wu, S., Su, Z., Li, T., Liu, S., Gao, Y., Liu, J., Feng, X., Wang, W., Zhao, H., Cui, Z., Li, G., Meng, F."3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression". Experimental and Therapeutic Medicine 17.3 (2019): 1593-1600.
Chicago
Guo, J., Zhao, C., Yao, R., Sui, A., Sun, L., Liu, X., Wu, S., Su, Z., Li, T., Liu, S., Gao, Y., Liu, J., Feng, X., Wang, W., Zhao, H., Cui, Z., Li, G., Meng, F."3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1593-1600. https://doi.org/10.3892/etm.2019.7153
Copy and paste a formatted citation
x
Spandidos Publications style
Guo J, Zhao C, Yao R, Sui A, Sun L, Liu X, Wu S, Su Z, Li T, Liu S, Liu S, et al: 3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression. Exp Ther Med 17: 1593-1600, 2019.
APA
Guo, J., Zhao, C., Yao, R., Sui, A., Sun, L., Liu, X. ... Meng, F. (2019). 3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression. Experimental and Therapeutic Medicine, 17, 1593-1600. https://doi.org/10.3892/etm.2019.7153
MLA
Guo, J., Zhao, C., Yao, R., Sui, A., Sun, L., Liu, X., Wu, S., Su, Z., Li, T., Liu, S., Gao, Y., Liu, J., Feng, X., Wang, W., Zhao, H., Cui, Z., Li, G., Meng, F."3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression". Experimental and Therapeutic Medicine 17.3 (2019): 1593-1600.
Chicago
Guo, J., Zhao, C., Yao, R., Sui, A., Sun, L., Liu, X., Wu, S., Su, Z., Li, T., Liu, S., Gao, Y., Liu, J., Feng, X., Wang, W., Zhao, H., Cui, Z., Li, G., Meng, F."3D culture enhances chemoresistance of ALL Jurkat cell line by increasing DDR1 expression". Experimental and Therapeutic Medicine 17, no. 3 (2019): 1593-1600. https://doi.org/10.3892/etm.2019.7153
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