Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis

Liu,Guitao; Yu,Junfeng; Li,Cuidong; Zhou,Xiaoyun; Nie,Liping; Wei,Yanhua; Wang,Wenyu; Zhang,Ying; Nusilaiti,Nuziguli; Hua,Ping; Wang,Xiaohua; Wei,Wenlong; Li,Xinyan

doi:10.3892/etm.2019.7166

Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis

Authors:
- Guitao Liu
- Junfeng Yu
- Cuidong Li
- Xiaoyun Zhou
- Liping Nie
- Yanhua Wei
- Wenyu Wang
- Ying Zhang
- Nuziguli Nusilaiti
- Ping Hua
- Xiaohua Wang
- Wenlong Wei
- Xinyan Li
View Affiliations

Affiliations: Department of Occupational Disease Prevention and Control, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Dermatological Department, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Respiratory Diseases, The Fifth Affiliated Clinical Medical College of Xinjiang Medical University, Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China, Endocrinology Department, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China
Published online on: January 11, 2019 https://doi.org/10.3892/etm.2019.7166
Pages: 2172-2184
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study aimed to observe the identification of biomarkers of silicosis based on the differentially expressed serum proteins between normal healthy individuals and patients with silicosis fibrosis. A total number of 20 patients with clinically diagnosed silicosis were screened, which were designated as the foundation treatment group. In addition, 20 age‑matched healthy patients attending a check‑up at the physical examination department were selected. Serum samples were obtained and a combined protein chip with surface‑enhanced laser desorption ionization flight mass spectrometry was applied to perform serum analysis. Data preprocessing, screening differences in peak, hierarchical cluster analysis, Principal Component Analysis, construction of a decision tree model, and prediction based on the differences between peaks corresponding to proteins were performed to analyze the data. The results revealed differences in the proteins in serum between the normal group and the group prior to foundation treatment prediction. The corresponding names of the protein peak, predicted protein, and gene name were as follows: M1948_00, complement c3 frag, C3; M2017_02, amyloid‑βa4 protein, APP; and M2879_56, hepcidin, HAMP. Differentially expressed serum proteins in the normal group and the basis treatment group were predicted, including M2017_02, amyloid‑βa4 protein, APP; M2879_56, hepcidin, HAMP; and M3224_97, fibrinogen‑α chain frags, FGA. The differentially expressed serum proteins in the group prior to basis treatment and the group following basis treatment were predicted, including M2001_69, amyloid‑βa4 protein, APP; M2017_02, amyloid‑βa4 protein, APP, M4144_81, plasma protease c1 inhibitor frag, and SERPING1. In conclusion, there were differences in the proteins in serum between the patients with silicosis fibrosis and healthy individuals.

View Figures

View References

1	Jp NA, Imanaka M and Suganuma N: Japanese workplace health management in pneumoconiosis prevention. J Occup Health. 59:91–103. 2017. View Article : Google Scholar : PubMed/NCBI
2	Liu H, Tang Z, Yang Y, Weng D, Sun G, Duan Z and Chen J: Identification and classification of high risk groups for coal workers' Pneumoconiosis using an artificial neural network based on occupational histories: A retrospective cohort study. BMC Public Health. 9:3662009. View Article : Google Scholar : PubMed/NCBI
3	Fichtner-Feigl S, Fuss IJ, Young CA, Watanabe T, Geissler EK, Schlitt HJ, Kitani A and Strober W: Induction of IL-13 triggers TGF-beta1-dependent tissue fibrosis in chronic 2,4,6-trinitrobenzene sulfonic acid colitis. J Immunol. 178:5859–5870. 2007. View Article : Google Scholar : PubMed/NCBI
4	Mandal AK, Zhang Z, Ray R, Choi MS, Chowdhury B, Pattabiraman N and Mukherjee AB: Uteroglobin represses allergen-induced inflammatory response by blockingPGD2 receptor-mediated functions. J Exp Med. 199:1317–1330. 2004. View Article : Google Scholar : PubMed/NCBI
5	Ernst H, Rittinghausen S, Bartsch W, Creutzenberg O, Dasenbrock C, Görlitz BD, Hecht M, Kairies U, Muhle H, Müller M, et al: Pulmonary inflammation in rats after intratracheal instillation of quartz, amorphous SiO2, carbon black, and coal dust and the influence of poly-2-vinylpyridine-N-oxide (PVNO). Exp Toxicol Pathol. 54:109–126. 2002. View Article : Google Scholar : PubMed/NCBI
6	Jiang PR, Cao Z, Qiu ZL, Pan JW, Zhang N and Wu YF: Plasma levels of TNF-α and MMP-9 in patients with silicosis. Eur Rev Med Pharmacol Sci. 19:1716–1720. 2015.PubMed/NCBI
7	Lustgarten JL, Kimmel C, Ryberg H and Hogan W: EPO-KB: A searchable knowledge base of biomarker to protein links. Bioinformatics. 24:1418–1419. 2008. View Article : Google Scholar : PubMed/NCBI
8	Mahavadi P, Korfei M, Henneke I, Liebisch G, Schmitz G, Gochuico BR, Markart P, Bellusci S, Seeger W, Ruppert C and Guenther A: Epithelial stress and apoptosis underlie Hermansky-Pudlak syndrome-associated interstitial pneumonia. Am J Respir Crit Care Med. 182:207–219. 2010. View Article : Google Scholar : PubMed/NCBI
9	Carneiro PJ, Clevelario AL, Padilha GA, Silva JD, Kitoko JZ, Olsen PC, Capelozzi VL, Rocco PR and Cruz FF: Cruz bosutinib therapy ameliorates lung inflammation and fibrosis in experimental silicosis. Front Physiol. 8:1592017. View Article : Google Scholar : PubMed/NCBI
10	Liu H, Cheng Y, Yang J, Wang W, Fang S, Zhang W, Han B, Zhou Z, Yao H, Chao J and Liao H: BBC3 in macrophages promoted pulmonary fibrosis development through inducing autophagy during silicosis. Cell Death Dis. 8:e26572017. View Article : Google Scholar : PubMed/NCBI
11	Chen Y, Li C, Lu Y, Zhuang H, Gu W, Liu B, Liu F, Sun J, Yan B, Weng D and Chen J: IL-10-Producing CD1d hi CD5+ regulatory B cells may play a critical role in modulating immune homeostasis in silicosis patients. Front Immunol. 8:1102017. View Article : Google Scholar : PubMed/NCBI
12	Bhattacharya S, Dey A, Pal A, Kar S and Saha S: Silicosis in the form of progressive massive fibrosis: A diagnostic challenge. Indian J Occup Environ Med. 20:114–117. 2016. View Article : Google Scholar : PubMed/NCBI
13	Rosengarten D, Fox BD, Fireman E, Blanc PD, Rusanov V, Fruchter O, Raviv Y, Shtraichman O, Saute M and Kramer MR: Survival following lung transplantation for artificial stone silicosis relative to idiopathic pulmonary fibrosis. Am J Ind Med. 60:248–254. 2017. View Article : Google Scholar : PubMed/NCBI
14	Mouratis MA and Aidinis V: Modeling pulmonary fibrosis with bleomycin. Curr Opin Pulm Med. 17:355–361. 2011. View Article : Google Scholar : PubMed/NCBI
15	Chiba Y, Kurotani R, Kusakabe T, Miura T, Link BW, Misawa M and Kimura S: Uteroglobin-related protein 1 expression suppresses allergic airway inflammation in mice. Am J Respir Crit Care Med. 173:958–964. 2006. View Article : Google Scholar : PubMed/NCBI
16	Liu P, Wang SX, Chen L, Wei MT, Liang XC, Wang YF and Tu ZG: Changes of Clara cell protein and surfactant protein-D in serum of patients with silicosis. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 25:18–21. 2007.(In Chinese). PubMed/NCBI
17	Anlar HG, Bacanli M, İritaş S, Bal C, Kurt T, Tutkun E, Hinc Yilmaz O and Basaran N: Effects of occupational silica exposure on OXIDATIVE stress and immune system parameters in ceramic workers in TURKEY. J Toxicol Environ Health A. 80:688–696. 2017. View Article : Google Scholar : PubMed/NCBI
18	Zhu Z, Yang G, Wang Y, Yang J, Gao A, Niu P and Tian L: Suppression of thioredoxin system contributes to silica-induced oxidative stress and pulmonary fibrogenesis in rats. Toxicol Lett. 222:289–294. 2013. View Article : Google Scholar : PubMed/NCBI
19	Liu H, Fang S, Wang W, Cheng Y, Zhang Y, Liao H, Yao H and Chao J: Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy. Part Fibre Toxicol. 13:552016. View Article : Google Scholar : PubMed/NCBI
20	Fang SC, Zhang HT, Wang CY and Zhang YM: Serum CA125 and NSE: Biomarkers of disease severity in patients with silicosis. Clin Chim Acta. 433:123–127. 2014. View Article : Google Scholar : PubMed/NCBI
21	Lü GC, Yao JM and Zhang JW: Significance of detection of serum oxidant function in patients with silicosis. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 28:52–53. 2010.(In Chinese). PubMed/NCBI
22	Idec-Sadkowska I, Andrzejak R, Antonowicz-Juchniewicz J and Kaczmarek-Wdowiak B: Trials of casual treatment of silicosis. Med Pr. 57:271–280. 2006.(In Polish). PubMed/NCBI