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Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis

  • Authors:
    • Guitao Liu
    • Junfeng Yu
    • Cuidong Li
    • Xiaoyun Zhou
    • Liping Nie
    • Yanhua Wei
    • Wenyu Wang
    • Ying Zhang
    • Nuziguli Nusilaiti
    • Ping Hua
    • Xiaohua Wang
    • Wenlong Wei
    • Xinyan Li
  • View Affiliations / Copyright

    Affiliations: Department of Occupational Disease Prevention and Control, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Dermatological Department, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Respiratory Diseases, The Fifth Affiliated Clinical Medical College of Xinjiang Medical University, Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China, Endocrinology Department, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2172-2184
    |
    Published online on: January 11, 2019
       https://doi.org/10.3892/etm.2019.7166
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Abstract

The present study aimed to observe the identification of biomarkers of silicosis based on the differentially expressed serum proteins between normal healthy individuals and patients with silicosis fibrosis. A total number of 20 patients with clinically diagnosed silicosis were screened, which were designated as the foundation treatment group. In addition, 20 age‑matched healthy patients attending a check‑up at the physical examination department were selected. Serum samples were obtained and a combined protein chip with surface‑enhanced laser desorption ionization flight mass spectrometry was applied to perform serum analysis. Data preprocessing, screening differences in peak, hierarchical cluster analysis, Principal Component Analysis, construction of a decision tree model, and prediction based on the differences between peaks corresponding to proteins were performed to analyze the data. The results revealed differences in the proteins in serum between the normal group and the group prior to foundation treatment prediction. The corresponding names of the protein peak, predicted protein, and gene name were as follows: M1948_00, complement c3 frag, C3; M2017_02, amyloid‑βa4 protein, APP; and M2879_56, hepcidin, HAMP. Differentially expressed serum proteins in the normal group and the basis treatment group were predicted, including M2017_02, amyloid‑βa4 protein, APP; M2879_56, hepcidin, HAMP; and M3224_97, fibrinogen‑α chain frags, FGA. The differentially expressed serum proteins in the group prior to basis treatment and the group following basis treatment were predicted, including M2001_69, amyloid‑βa4 protein, APP; M2017_02, amyloid‑βa4 protein, APP, M4144_81, plasma protease c1 inhibitor frag, and SERPING1. In conclusion, there were differences in the proteins in serum between the patients with silicosis fibrosis and healthy individuals.
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Copy and paste a formatted citation
Spandidos Publications style
Liu G, Yu J, Li C, Zhou X, Nie L, Wei Y, Wang W, Zhang Y, Nusilaiti N, Hua P, Hua P, et al: Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis. Exp Ther Med 17: 2172-2184, 2019.
APA
Liu, G., Yu, J., Li, C., Zhou, X., Nie, L., Wei, Y. ... Li, X. (2019). Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis. Experimental and Therapeutic Medicine, 17, 2172-2184. https://doi.org/10.3892/etm.2019.7166
MLA
Liu, G., Yu, J., Li, C., Zhou, X., Nie, L., Wei, Y., Wang, W., Zhang, Y., Nusilaiti, N., Hua, P., Wang, X., Wei, W., Li, X."Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis". Experimental and Therapeutic Medicine 17.3 (2019): 2172-2184.
Chicago
Liu, G., Yu, J., Li, C., Zhou, X., Nie, L., Wei, Y., Wang, W., Zhang, Y., Nusilaiti, N., Hua, P., Wang, X., Wei, W., Li, X."Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2172-2184. https://doi.org/10.3892/etm.2019.7166
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G, Yu J, Li C, Zhou X, Nie L, Wei Y, Wang W, Zhang Y, Nusilaiti N, Hua P, Hua P, et al: Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis. Exp Ther Med 17: 2172-2184, 2019.
APA
Liu, G., Yu, J., Li, C., Zhou, X., Nie, L., Wei, Y. ... Li, X. (2019). Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis. Experimental and Therapeutic Medicine, 17, 2172-2184. https://doi.org/10.3892/etm.2019.7166
MLA
Liu, G., Yu, J., Li, C., Zhou, X., Nie, L., Wei, Y., Wang, W., Zhang, Y., Nusilaiti, N., Hua, P., Wang, X., Wei, W., Li, X."Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis". Experimental and Therapeutic Medicine 17.3 (2019): 2172-2184.
Chicago
Liu, G., Yu, J., Li, C., Zhou, X., Nie, L., Wei, Y., Wang, W., Zhang, Y., Nusilaiti, N., Hua, P., Wang, X., Wei, W., Li, X."Application of protein chip combined with SELDI‑TOF‑MS detection to investigate serum protein expression in patients with silicosis fibrosis". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2172-2184. https://doi.org/10.3892/etm.2019.7166
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