Anti‑bacterial activity of mutant chensinin‑1 peptide against multidrug‑resistant Pseudomonas aeruginosa and its effects on biofilm‑associated gene expression

Yu,Zhiyuan; Kong,Yi; Luo,Zhenqin; Liu,Tongtong; Lin,Jinguan

doi:10.3892/etm.2019.7182

Anti‑bacterial activity of mutant chensinin‑1 peptide against multidrug‑resistant Pseudomonas aeruginosa and its effects on biofilm‑associated gene expression

Authors:
- Zhiyuan Yu
- Yi Kong
- Zhenqin Luo
- Tongtong Liu
- Jinguan Lin
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Affiliations: Department of Comprehensive Chemotherapy, Hunan Cancer Hospital, Affiliated Cancer Hospital of Xiangya Medical School of Central South University, Changsha, Hunan 410006, P.R. China, Department of Biotechnology, School of Life Science, Liaoning Normal University, Dalian, Liaoning 116081, P.R. China
Published online on: January 17, 2019 https://doi.org/10.3892/etm.2019.7182
Pages: 2031-2038
Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Nosocomial infections with Pseudomonas aeruginosa (PA) are difficult to treat due to the low outer membrane permeability of the bacterium and the development of resistance. In the present study, the anti‑microbial peptide (AMP) mutant chensinin‑1 (MC1) was revealed to exhibit anti‑bacterial activity against a multidrug‑resistant PA (MRPA) strain in vitro, and the minimum inhibitory concentration was 25 µM, which was 4‑fold higher than that of the native strain. MC1 was able to disrupt the integrity of the cytoplasmic membrane in the native PA strain and MRPA and had a similar membrane depolarization ability in these strains, but the outer membrane permeability of MRPA cells was lower than that of native PA cells, as determined by a 1‑N‑phenylnaphthylamine assay. In addition, the abundance of the gene Psl encoding for biofilm‑associated polysaccharides was detected using Congo red, and a high concentration of MC1 inhibited the formation of MRPA biofilms. Furthermore, the expression levels of biofilm‑associated genes affected by the AMP, MC1, were quantified by polymerase chain reaction analysis. The results indicated that MC1 induced biofilm inhibition by downregulating the relative expression of specific biofilm polysaccharide‑associated genes, including pelA, algD and pslA. The present results indicated that the AMP MC1 may be an effective antibiotic against MRPA strains.

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