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Article

Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells

  • Authors:
    • Shu Yang
    • Hong Jin
    • Zhi‑Gang Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, P.R. China, Disinfection Evaluation Research Center, Institute of Disease Prevention and Control of PLA, Beijing 100071, P.R. China
  • Pages: 2397-2402
    |
    Published online on: January 21, 2019
       https://doi.org/10.3892/etm.2019.7186
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Abstract

Tight junctions (TJs) serve an important role in maintaining the integrity of the blood‑brain barrier (BBB), while neurological disorders, including ischemic stroke, induce TJ disruption and increase BBB permeability; results include edema formation and hemorrhage transformation. Cerebral endothelium protection presents a promising approach in ischemic stroke therapy. In the current study, protective effects of the epidermal growth factor (EGF) on ischemia‑induced disruption of BBB integrity were examined using an oxygen‑glucose deprivation (OGD) model in bEnd3 cells. Expression levels of claudin‑5 and TJ protein‑1 (ZO‑1) were determined by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Cell viability was evaluated by cell counting kit‑8 assay and the endothelial permeability of Lucifer yellow (LY) was assessed using Transwell assays. The results revealed that post‑ischemia administration of EGF (250 ng/ml) significantly attenuated the decrease in mRNA (P<0.05) and protein (P<0.01) expression levels of claudin‑5 and ZO‑1, and the increase in endothelial permeability of LY (P<0.05) induced by 4 h OGD exposure followed by 24 h reoxygenation. In addition, EGF did not significant affect cell viability. The current study suggested a potential of EGF to improve BBB integrity against ischemic injury by upregulating the expression of TJ proteins and reducing endothelial permeability.
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1 

Yang S, Mei S, Jin H, Zhu B, Tian Y, Huo J, Cui X, Guo A and Zhao Z: Identification of two immortalized cell lines, ECV304 and bEnd3, for in vitro permeability studies of blood-brain barrier. PLoS One. 12:e01870172017. View Article : Google Scholar : PubMed/NCBI

2 

Cecchelli R, Berezowski V, Lundquist S, Culot M, Renftel M, Dehouck MP and Fenart L: Modelling of the blood-brain barrier in drug discovery and development. Nat Rev Drug Discov. 6:650–661. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Cardoso FL, Brites D and Brito MA: Looking at the blood-brain barrier: Molecular anatomy and possible investigation approaches. Brain Res Rev. 64:328–363. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Ronaldson PT and Davis TP: Blood-brain barrier integrity and glial support: Mechanisms that can be targeted for novel therapeutic approaches in stroke. Curr Pharm Des. 18:3624–3644. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Liu X, Zhu X, Chen M, Ge Q, Shen Y and Pan S: Resveratrol protects PC12 cells against OGD/R-induced apoptosis via the mitochondrial-mediated signaling pathway. Acta Biochim Biophys Sin (Shanghai). 48:342–353. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Khatri R, McKinney AM, Swenson B and Janardhan V: Blood-brain barrier, reperfusion injury, and hemorrhagic transformation in acute ischemic stroke. Neurology. 79 (13 Suppl 1):S52–S57. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Warach S and Latour LL: Evidence of reperfusion injury, exacerbated by thrombolytic therapy, in human focal brain ischemia using a novel imaging marker of early blood-brain barrier disruption. Stroke. 35 (11 Suppl 1):S2659–S2661. 2004. View Article : Google Scholar

8 

Zhu H, Wang Z, Xing Y, Gao Y, Ma T, Lou L, Lou J, Gao Y, Wang S and Wang Y: Baicalin reduces the permeability of the blood-brain barrier during hypoxia in vitro by increasing the expression of tight junction proteins in brain microvascular endothelial cells. J Ethnopharmacol. 141:714–720. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Xie R, Li X, Ling Y, Shen C, Wu X, Xu W and Gao X: Alpha-lipoic acid pre- and post-treatments provide protection against in vitro ischemia-reperfusion injury in cerebral endothelial cells via Akt/mTOR signaling. Brain Res. 1482:81–90. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Cao G, Jiang N, Hu Y, Zhang Y, Wang G, Yin M, Ma X, Zhou K, Qi J, Yu B and Kou J: Ruscogenin attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP/NLRP3 inflammasome activation and the MAPK pathway. Int J Mol Sci. 17(pii): E14182016. View Article : Google Scholar : PubMed/NCBI

11 

Fu S, Gu Y, Jiang JQ, Chen X, Xu M, Chen X and Shen J: Calycosin-7-O-β-D-glucoside regulates nitric oxide/caveolin-1/matrix metalloproteinases pathway and protects blood-brain barrier integrity in experimental cerebral ischemia-reperfusion injury. J Ethnopharmacol. 155:692–701. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Pillai DR, Shanbhag NC, Dittmar MS, Boqdahn U and Schlachetzki F: Neurovascular protection by targeting early blood-brain barrier disruption with neurotrophic factors after ischemia-reperfusion in rats*. J Cereb Blood Flow Metab. 33:557–566. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Plata-Salamán CR: Epidermal growth factor and the nervous system. Peptides. 12:653–663. 1991. View Article : Google Scholar : PubMed/NCBI

14 

Wang YF, Cooke MJ, Sachewsky N, Morshead CM and Shoichet MS: Bioengineered sequential growth factor delivery stimulates brain tissue regeneration after stroke. J Control Release. 172:1–11. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Liu W, Wang P, Shang C, Chen L, Cai H, Ma J, Yao Y, Shang X and Xue Y: Endophilin-1 regulates blood-brain barrier permeability by controlling ZO-1 and occludin expression via the EGFR-ERK1/2 pathway. Brain Res. 1573:17–26. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Chen L, Liu W, Wang P, Xue Y, Su Q, Zeng C and Shang X: Endophilin-1 regulates blood-brain barrier permeability via EGFR-JNK signaling pathway. Brain Res. 1606:44–53. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Ronaldson PT and Davis TP: Targeting blood-brain barrier changes during inflammatory pain: An opportunity for optimizing CNS drug delivery. Ther Deliv. 2:1015–1041. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Wilhelm I and Krizbai IA: In vitro models of the blood-brain barrier for the study of drug delivery to the brain. Mol Pharm. 11:1949–1963. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Choi KH, Kim HS, Park MS, Kim JT, Kim JH, Cho KA, Lee MC, Lee HJ and Cho KH: Regulation of caveolin-1 expression determines early brain edema after experimental focal cerebral ischemia. Stroke. 47:1336–1343. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Guo J, Krause DN, Horne J, Weiss JH, Li X and Duckles SP: Estrogen-receptor-mediated protection of cerebral endothelial cell viability and mitochondrial function after ischemic insult in vitro. J Cereb Blood Flow Metab. 30:545–554. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Liu J, Jin X, Liu KJ and Liu W: Matrix metalloproteinase-2-mediated occludin degradation and caveolin-1-mediated claudin-5 redistribution contribute to blood-brain barrier damage in early ischemic stroke stage. J Neurosci. 32:3044–3057. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Rahman NA, Rasil ANHM, Meyding-Lamade U, Craemer EM, Diah S, Tuah AA and Muharram SH: Immortalized endothelial cell lines for in vitro blood-brain barrier models: A systematic review. Brain Res. 1642:532–545. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Ku JM, Taher M, Chin KY, Grace M, McIntyre P and Miller AA: Characterization of a mouse cerebral microvascular endothelial cell line (bEnd.3) after oxygen glucose deprivation and reoxygenation. Clin Exp Pharmacol Physiol. 43:777–786. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Igarashi Y, Utsumi H, Chiba H, Yamada-Sasamori Y, Tobioka H, Kamimura Y, Furuuchi K, Kokai Y, Nakagawa T, Mori M and Sawada N: Glial cell line-derived neurotrophic factor induces barrier function of endothelial cells forming the blood-brain barrier. Biochem Biophys Res Commun. 261:108–112. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Abbott NJ, Rönnbäck L and Hansson E: Astrocyte-endothelial interactions at the blood-brain barrier. Nat Rev Neurosci. 7:41–53. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Basuroy S, Seth A, Elias B, Naren AP and Rao R: MAPK interacts with occludin and mediates EGF-induced prevention of tight junction disruption by hydrogen peroxide. Biochem J. 393:69–77. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Park JH and Han HJ: Caveolin-1 plays important role in EGF-induced migration and proliferation of mouse embryonic stem cells: Involvement of PI3K/Akt and ERK. Am J Physiol Cell Physiol. 297:C935–C944. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Campbell M, Collery R, McEvoy A, Gardiner TA, Stitt AW and Brankin B: Involvement of MAPKs in endostatin-mediated regulation of blood-retinal barrier function. Curr Eye Res. 31:1033–1045. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Sumanasekera WK, Sumanasekera GU, Mattingly KA, Dougherty SM, Keynton RS and Klinge CM: Estradiol and dihydrotestosterone regulate endothelial cell barrier function after hypergravity-induced alterations in MAPK activity. Am J Physiol Cell Physiol. 293:C566–C573. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Luo D, Zhao J and Rong J: Plant-derived triterpene celastrol ameliorates oxygen glucose deprivation-induced disruption of endothelial barrier assembly via inducing tight junction proteins. Phytomedicine. 23:1621–1628. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Lu D, Mai HC, Liang YB, Xu BD, Xu AD and Zhang YS: Beneficial role of rosuvastatin in blood-brain barrier damage following experimental ischemic stroke. Front Pharmacol. 9:9262018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang S, Jin H and Zhao ZG: Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells. Exp Ther Med 17: 2397-2402, 2019.
APA
Yang, S., Jin, H., & Zhao, Z. (2019). Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells. Experimental and Therapeutic Medicine, 17, 2397-2402. https://doi.org/10.3892/etm.2019.7186
MLA
Yang, S., Jin, H., Zhao, Z."Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells". Experimental and Therapeutic Medicine 17.3 (2019): 2397-2402.
Chicago
Yang, S., Jin, H., Zhao, Z."Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2397-2402. https://doi.org/10.3892/etm.2019.7186
Copy and paste a formatted citation
x
Spandidos Publications style
Yang S, Jin H and Zhao ZG: Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells. Exp Ther Med 17: 2397-2402, 2019.
APA
Yang, S., Jin, H., & Zhao, Z. (2019). Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells. Experimental and Therapeutic Medicine, 17, 2397-2402. https://doi.org/10.3892/etm.2019.7186
MLA
Yang, S., Jin, H., Zhao, Z."Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells". Experimental and Therapeutic Medicine 17.3 (2019): 2397-2402.
Chicago
Yang, S., Jin, H., Zhao, Z."Epidermal growth factor treatment has protective effects on the integrity of the blood‑brain barrier against cerebral ischemia injury in bEnd3 cells". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2397-2402. https://doi.org/10.3892/etm.2019.7186
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