Downregulation of microRNA‑374a predicts poor prognosis in human glioma

  • Authors:
    • Qiang Dong
    • Guoqiang Yuan
    • Min Liu
    • Qiqi Xie
    • Jianhong Hu
    • Maolin Wang
    • Shangyu Liu
    • Xiaojun Ma
    • Yawen Pan
  • View Affiliations

  • Published online on: January 21, 2019     https://doi.org/10.3892/etm.2019.7190
  • Pages: 2077-2084
  • Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Certain microRNAs (miRNAs/miRs) may be used as prognostic biomarkers in various types of cancer. The purpose of the present study was to identify miRNAs that were abnormally expressed in glioma of different grades, and to evaluate their clinical implications in patients with glioma. The differentially expressed miRNAs were evaluated from the expression profiles of six glioma tissues (three low‑grade and three high‑grade gliomas) determined using a microarray platform. Reverse transcription‑quantitative polymerase chain reaction analysis was used to further verify the aberrant expression of the candidate miRNA in a set of 42 patients and 5 healthy controls. The miRNA target genes were predicted and the protein‑protein interaction network was generated; furthermore, functional enrichment analysis of the target genes in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways was performed. Kaplan‑Meier curves and Log‑rank analysis, as well as multivariate Cox regression analysis were performed to assess the association of the candidate miRNA with patient survival. A total of 15 differentially expressed miRNAs, including 13 downregulated and 2 upregulated miRNAs, were identified by comparison of low‑grade and high‑grade glioma tissues. The miR‑374a expression of high‑grade gliomas was significantly lower than that of low‑grade gliomas (fold change, ‑4.43; P=0.027). The expression levels of miR‑374a gradually decreased with the increase of the pathological grade of glioma. Pearson's Chi‑square test was used to determine the association of miR‑374a expression with several clinicopathological factors. Furthermore, low expression of miR‑374a was determined to be an independent prognostic marker and that it was significantly associated with overall survival (P=0.0213). GO and KEGG pathway analysis revealed that the target genes of miR‑374a may be involved in the regulation of the RNA polymerase II promoter and mTOR signaling pathway. The four hub genes (CCND1, SP1, CDK4, CDK6) were also identified by PPI network analysis. In conclusion, the present study indicated that miR‑374a may be used as a promising prognostic biomarker for the screening of high‑risk populations and for the assessment of the prognosis of patients with glioma.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Dong Q, Yuan G, Liu M, Xie Q, Hu J, Wang M, Liu S, Ma X and Pan Y: Downregulation of microRNA‑374a predicts poor prognosis in human glioma. Exp Ther Med 17: 2077-2084, 2019
APA
Dong, Q., Yuan, G., Liu, M., Xie, Q., Hu, J., Wang, M. ... Pan, Y. (2019). Downregulation of microRNA‑374a predicts poor prognosis in human glioma. Experimental and Therapeutic Medicine, 17, 2077-2084. https://doi.org/10.3892/etm.2019.7190
MLA
Dong, Q., Yuan, G., Liu, M., Xie, Q., Hu, J., Wang, M., Liu, S., Ma, X., Pan, Y."Downregulation of microRNA‑374a predicts poor prognosis in human glioma". Experimental and Therapeutic Medicine 17.3 (2019): 2077-2084.
Chicago
Dong, Q., Yuan, G., Liu, M., Xie, Q., Hu, J., Wang, M., Liu, S., Ma, X., Pan, Y."Downregulation of microRNA‑374a predicts poor prognosis in human glioma". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2077-2084. https://doi.org/10.3892/etm.2019.7190