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Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy

  • Authors:
    • Changtai Wang
    • Shu Yu
    • Yafei Zhang
    • Min Zhang
    • Liying Lv
    • Cheng Huang
    • Xu Li
    • Jun Li
    • Zhenhua Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, P.R. China, Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, College of Pharmacy, Anhui Medical University, Hefei, Anhui 230022, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2473-2484
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    Published online on: February 11, 2019
       https://doi.org/10.3892/etm.2019.7255
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Abstract

The association between hepatitis B virus (HBV) quasispecies (QS) and the efficacy of nucleos(t)ide analog therapy is currently not well defined, particularly in the case of lamivudine (LAM)/adefovir (ADV) combination rescue therapy for patients with chronic HBV infection (CHB) presenting with LAM resistance. In the present study, 16 CHB patients with the rtM204I/V mutation in the tyrosine-methionine-aspartate‑aspartate motif of the C domain of the polymerase gene who switched to LAM/ADV treatment due to LAM resistance were assessed. HBV DNA was isolated from these patients and the reverse transcriptase (RT) region was sequenced. The QS heterogeneity and distribution was analyzed, the mutation sites were recorded and the phylogenetic trees were constructed. The results indicated that QS heterogeneity and distribution in the RT and S regions were not significantly different between responders (RS) and non‑RS (NRS) at baseline (P>0.05), except for the higher frequency of a dominant strain in the RT region at the nucleotide level in the RS group (P=0.039). In addition, in NRS, no significant difference in QS heterogeneity or distribution in these regions was identified at six months vs. the baseline. Furthermore, although in the non‑responder group the frequency of the LAM resistance‑associated mutations (rtM204V/I) decreased at 6 months compared with the baseline, it did not disappear in any of the patients after six months of treatment. Analysis of individual patients did not indicate any consistent selection of specific HBV mutants during LAM/ADV rescue therapy. In conclusion, the baseline HBV QS within the RT and S regions may not be a valid predictor of the response to LAM/ADV rescue treatment in CHB patients with LAM resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Yu S, Zhang Y, Zhang M, Lv L, Huang C, Li X, Li J and Zhang Z: Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy. Exp Ther Med 17: 2473-2484, 2019.
APA
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C. ... Zhang, Z. (2019). Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy. Experimental and Therapeutic Medicine, 17, 2473-2484. https://doi.org/10.3892/etm.2019.7255
MLA
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C., Li, X., Li, J., Zhang, Z."Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy". Experimental and Therapeutic Medicine 17.4 (2019): 2473-2484.
Chicago
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C., Li, X., Li, J., Zhang, Z."Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2473-2484. https://doi.org/10.3892/etm.2019.7255
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Yu S, Zhang Y, Zhang M, Lv L, Huang C, Li X, Li J and Zhang Z: Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy. Exp Ther Med 17: 2473-2484, 2019.
APA
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C. ... Zhang, Z. (2019). Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy. Experimental and Therapeutic Medicine, 17, 2473-2484. https://doi.org/10.3892/etm.2019.7255
MLA
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C., Li, X., Li, J., Zhang, Z."Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy". Experimental and Therapeutic Medicine 17.4 (2019): 2473-2484.
Chicago
Wang, C., Yu, S., Zhang, Y., Zhang, M., Lv, L., Huang, C., Li, X., Li, J., Zhang, Z."Viral quasispecies of hepatitis B virus in patients with YMDD mutation and lamivudine resistance may not predict the efficacy of lamivudine/adefovir rescue therapy". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2473-2484. https://doi.org/10.3892/etm.2019.7255
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