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MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7

  • Authors:
    • Haiyan Guo
    • Linyun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Medicine, Fenyang College, Shanxi Medical University, Fenyang, Shanxi 032200, P.R. China, Shanxi Fenyang Prison Hospital, Fenyang, Shanxi 032200, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 209-215
    |
    Published online on: April 30, 2019
       https://doi.org/10.3892/etm.2019.7532
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Abstract

microRNA (miRNA/miR)‑30a, a tumor‑associated miRNA, has been implicated in the tumorigenesis and progression of different types of human cancer. Thyroid cancer is a common endocrine malignancy, of which papillary thyroid cancer (PTC) accounts for ~80‑90% of all TC. However, the effect of miR‑30a in PTC is yet to be fully elucidated. The TPC‑1 human PTC cell line, as well as the normal human thyroid cell line (HT‑ori3), were utilized in the current study. The PTC cell line was transfected with a miR‑30a mimic. Subsequently, reverse transcription‑quantitative polymerase chain reaction was performed to detect the expression of miR‑30a and E2F transcription factor 7 (E2F7). Cell proliferation was assessed via a MTT assay and transwell migration and invasion assays were performed to detect the migration and invasion of PTC cells. A dual‑luciferase reporter assay was also utilized to clarify the association between miR‑30a and E2F7. The results of the current study revealed that miR‑30a was significantly downregulated in TPC‑1 cells compared with HT‑ori3 cells and that the expression of E2F7 was significantly upregulated in PTC cells. The upregulation of miR‑30a also inhibited the proliferation, migration and invasion of PTC cells. Furthermore, the luciferase assay revealed that miR‑30a binds to the 3'‑UTR of E2F7. Additionally, the overexpression of miR‑30a decreased E2F7 levels in TPC‑1 cells. These results indicate that miR‑30a functions as a tumor suppressor in PTC by direct targeting E2F7 and that miR‑30a may be a novel therapeutic target for patients with PTC.
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Copy and paste a formatted citation
Spandidos Publications style
Guo H and Zhang L: MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7. Exp Ther Med 18: 209-215, 2019.
APA
Guo, H., & Zhang, L. (2019). MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7. Experimental and Therapeutic Medicine, 18, 209-215. https://doi.org/10.3892/etm.2019.7532
MLA
Guo, H., Zhang, L."MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7". Experimental and Therapeutic Medicine 18.1 (2019): 209-215.
Chicago
Guo, H., Zhang, L."MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7". Experimental and Therapeutic Medicine 18, no. 1 (2019): 209-215. https://doi.org/10.3892/etm.2019.7532
Copy and paste a formatted citation
x
Spandidos Publications style
Guo H and Zhang L: MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7. Exp Ther Med 18: 209-215, 2019.
APA
Guo, H., & Zhang, L. (2019). MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7. Experimental and Therapeutic Medicine, 18, 209-215. https://doi.org/10.3892/etm.2019.7532
MLA
Guo, H., Zhang, L."MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7". Experimental and Therapeutic Medicine 18.1 (2019): 209-215.
Chicago
Guo, H., Zhang, L."MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7". Experimental and Therapeutic Medicine 18, no. 1 (2019): 209-215. https://doi.org/10.3892/etm.2019.7532
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