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Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head

  • Authors:
    • Yungang Wu
    • Chunwu Zhang
    • Jianjing Wu
    • Yan Han
    • Chunlei Wu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 163-171
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    Published online on: May 8, 2019
       https://doi.org/10.3892/etm.2019.7556
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Abstract

The present study aimed to explore the potential of combined treatment with mesenchymal stem cells (MSCs) and danshen for angiogenesis and bone regeneration in a rabbit model of avascular necrosis of femoral head (ANFH). A rabbit model of ANFH was established using the Shwartzman reaction with methylprednisolone and Escherichia coli endotoxin injection. Magnetic resonance imaging (MRI) and histopathological examination were used to evaluate the rabbit model of ANFH. The rabbits were randomly divided into the danshen group, the MSCs group, the danshen combined with MSCs group and the model group (treated with physiological saline). The expression level of monocyte chemoattractant protein‑1 (MCP‑1) and stromal cell‑derived factor‑1 (SDF‑1) were determined by reverse transcription polymerase chain reaction (RT‑PCR). The expression level of bone morphogenetic protein‑2 (BMP‑2) and vascular endothelial growth factor (VEGF) were detected by immunofluorescence and the mRNA expression of BMP‑2 and VEGF were detected by RT‑PCR. Typical osteonecrosis occurred in the rabbit model of ANFH, which indicated that the model was successfully established. MCP‑1 and SDF‑1 were significantly increased in the model group compared with the normal group (P<0.05). Following the administration of MSCs and Salvia miltiorrhiza (danshen), MSCs labeled with 5‑bromo‑2‑deoxyuridine were observed to be gathered in the necrotic area. The increased migration of MSCs to the necrotic area may be due to the upregulated expression of the chemokines MCP‑1 and SDF‑1. ANFH treated with danshen combined with MSCs may promote revascularization by increasing the expression of VEGF and BMP‑2 in the femoral head, promoting re‑ossification and revascularization. Danshen combined with the transplantation of MSCs may be regarded as a novel therapy for the treatment of ANFH in a clinical setting.
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Copy and paste a formatted citation
Spandidos Publications style
Wu Y, Zhang C, Wu J, Han Y and Wu C: Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head. Exp Ther Med 18: 163-171, 2019.
APA
Wu, Y., Zhang, C., Wu, J., Han, Y., & Wu, C. (2019). Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head. Experimental and Therapeutic Medicine, 18, 163-171. https://doi.org/10.3892/etm.2019.7556
MLA
Wu, Y., Zhang, C., Wu, J., Han, Y., Wu, C."Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head". Experimental and Therapeutic Medicine 18.1 (2019): 163-171.
Chicago
Wu, Y., Zhang, C., Wu, J., Han, Y., Wu, C."Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head". Experimental and Therapeutic Medicine 18, no. 1 (2019): 163-171. https://doi.org/10.3892/etm.2019.7556
Copy and paste a formatted citation
x
Spandidos Publications style
Wu Y, Zhang C, Wu J, Han Y and Wu C: Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head. Exp Ther Med 18: 163-171, 2019.
APA
Wu, Y., Zhang, C., Wu, J., Han, Y., & Wu, C. (2019). Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head. Experimental and Therapeutic Medicine, 18, 163-171. https://doi.org/10.3892/etm.2019.7556
MLA
Wu, Y., Zhang, C., Wu, J., Han, Y., Wu, C."Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head". Experimental and Therapeutic Medicine 18.1 (2019): 163-171.
Chicago
Wu, Y., Zhang, C., Wu, J., Han, Y., Wu, C."Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with danshen in a rabbit model of avascular necrotic femoral head". Experimental and Therapeutic Medicine 18, no. 1 (2019): 163-171. https://doi.org/10.3892/etm.2019.7556
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