Lazaroid U‑74389G in liver ischemia‑reperfusion injury: A swine model

Korontzi,Maria I.; Theodoropoulos,George; Agrogiannis,George; Flessas,Ioannis; Chrysikos,Dimosthenis; Gioxari,Aristea; Sergentanis,Theodoros  N.; Patsouris,Efstratios; Zografos,George C.; Papalois,Apostolos

doi:10.3892/etm.2019.7561

Lazaroid U‑74389G in liver ischemia‑reperfusion injury: A swine model

Authors:
- Maria I. Korontzi
- George Theodoropoulos
- George Agrogiannis
- Ioannis Flessas
- Dimosthenis Chrysikos
- Aristea Gioxari
- Theodoros N. Sergentanis
- Efstratios Patsouris
- George C. Zografos
- Apostolos Papalois
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Affiliations: First Department of Propaedeutic Surgery, Hippocration Hospital, School of Medicine, University of Athens, 11527 Athens, Greece, Department of Pathology, School of Medicine, University of Athens, 11527 Athens, Greece, Department of Nutrition and Dietetics, Harokopio University, 17676 Athens, Greece, Department of Hygiene, Epidemiology and Medical Statistics, School of Medicine, University of Athens, 11527 Athens, Greece, Experimental, Educational and Research Center, ELPEN Pharmaceuticals, 19009 Athens, Greece
Published online on: May 8, 2019 https://doi.org/10.3892/etm.2019.7561
Pages: 230-236

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Abstract

Reactive oxygen species have a key role in liver ischemia‑reperfusion (I/R) injury. In the present study, the effect of the anti‑oxidant compound lazaroid U‑74389G in preventing liver I/R injury was investigated in a swine model. Ischemia was produced by portal vein occlusion. Two sets of experiments were performed, each with two groups (n=7 per group). In the first group, the potential protective effect of an intracaval injection of U‑74389G after a 30‑min ischemia, followed by a 60‑min reperfusion period was assessed (biopsies at 0, 15, 30 and 90 min experimental time). In the second set, the effect of intracaval U‑74389G injection after 30 min of ischemia, followed by a longer reperfusion period of 120 min was determined (biopsies at 0, 15, 30 and 150 min experimental time). Liver malondialdehyde, hepatocyte vacuolation‑degeneration, venous congestion, inflammatory cell infiltration, sinus congestion‑dilation and Chiu score of intestinal damage were determined at up to 150 min of reperfusion. In the second set of experiments, the Chiu score of intestinal damage was improved by the administration of U‑74389G (3.17±0.40 vs. 4.33±0.21; P=0.030). However, in the two sets of experiments, the liver inflammatory reaction was more pronounced in the U‑74389G groups (P=0.017 for the first set, P=0.021 for the second set). No significant effect of U‑74389G on any other parameters was detected. In conclusion, intestinal damage due to portal venous congestion and reflow appears to be mitigated by the lazaroid U‑74389G; however, intracaval administration of U‑74389G does not appear to exert any protective effects against liver I/R‑induced inflammation.

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