MicroRNA‑34a suppresses the invasion and migration of colorectal cancer cells by enhancing EGR1 and inhibiting vimentin

Zhu,Wei; Long,Jia‑Li; Yin,Yu‑Ting; Guo,Hai‑Na; Jiang,En‑Ping; Li,Yu‑Ling; He,Qing‑Lian; Zeng,Chao; Sun,Yan‑Qin

doi:10.3892/etm.2019.7826

MicroRNA‑34a suppresses the invasion and migration of colorectal cancer cells by enhancing EGR1 and inhibiting vimentin

Authors:
- Wei Zhu
- Jia‑Li Long
- Yu‑Ting Yin
- Hai‑Na Guo
- En‑Ping Jiang
- Yu‑Ling Li
- Qing‑Lian He
- Chao Zeng
- Yan‑Qin Sun
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Affiliations: Department of Pathology, Guangdong Medical University, Dongguan, Guangdong 523808, P.R. China, Department of Pathology, Dongguan Maternal and Child Health Hospital, Dongguan, Guangdong 523002, P.R. China, Department of Pathology, Dongguan Hospital of Southern Medical University, Dongguan, Guangdong 523059, P.R. China
Published online on: July 30, 2019 https://doi.org/10.3892/etm.2019.7826
Pages: 2459-2466
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) are small non‑coding RNAs that serve a post‑transcriptional regulatory role in eukaryotes. Previous studies have demonstrated that the expression of miR‑34a in colorectal cancer (CRC) tissues is decreased compared with that in normal colorectal tissues. However, the role of miR‑34a in the invasion and metastasis of CRC remains unclear. In the present study, the levels of miR‑34a expression were measured in various CRC cell lines. The cells were transfected with miR‑34a mimics or inhibitors in order to assess the proliferation rate, and the colony forming, invasive and migratory abilities. Furthermore, the protein expression levels of vimentin and early growth response protein 1 (EGR1) were examined by western blot analysis. The results revealed that the expression of miR‑34a was low in SW620, RKO, LoVo and Caco‑2 cell lines and high in the SW480 and SW1116 cell lines. The migration, invasion and proliferation levels of SW480 cells were facilitated by decreasing the expression of miR‑34a. Transient transfection with miR‑34a mimics in SW620 cells caused a notable decrease in cell migration, invasion and proliferation levels compared with the control group, and a downregulation of vimentin and upregulation of EGR1 protein expression. The present study demonstrated that miR‑34a was deregulated in a highly invasive CRC cell lines, and that it may attenuate the migratory, invasive and proliferative capabilities of CRC cells by enhancing the expression of EGR1 and inhibiting that of vimentin. The results of the present study represent important progress towards understanding the mechanisms of CRC recurrence and metastasis.

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