The design of a homocysteine fluorescent probe based on Rhodamine B and its responsiveness in the serum of cerebral infarction patients

Zhao,Xiubao; Li,Xu; Ma,Ying; Zhu,Yu

doi:10.3892/etm.2019.7833

The design of a homocysteine fluorescent probe based on Rhodamine B and its responsiveness in the serum of cerebral infarction patients

Authors:
- Xiubao Zhao
- Xu Li
- Ying Ma
- Yu Zhu
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Affiliations: Department of Intensive Care Unit, Tianjin First Central Hospital, Tianjin 300192, P.R. China, Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin 300350, P.R. China, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, P.R. China
Published online on: July 30, 2019 https://doi.org/10.3892/etm.2019.7833
Pages: 2675-2680

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Abstract

High levels of homocysteine (Hcy) is closely associated with the onset of cerebral infarction. The present study aimed to synthesize a novel Hcy probe based on Rhodamine B, named S1‑4, a new compound that has not been previously reported. This probe exhibited good linear range under physiological fluid viscosity and pH; it has good selectivity for Hcy, and is able to avoid interference from other amino acids and metal ions. This probe can effectively measure the level of Hcy in the blood sera of healthy people and in patients with transient cerebral ischemia and cerebral infarction. However, satisfactory specificity and sensitivity to Hcy was not achieved according to receiver operating characteristic curve analysis. Overall, results from the present study suggested that following further optimization, this probe may be potentially applied in the diagnosis of cerebral infarction.

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