Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2019 Volume 18 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2019 Volume 18 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts

  • Authors:
    • Monica Benvenuto
    • Rosanna Mattera
    • Martino Tony Miele
    • Maria Gabriella Giganti
    • Ilaria Tresoldi
    • Loredana Albonici
    • Vittorio Manzari
    • Andrea Modesti
    • Laura Masuelli
    • Roberto Bei
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Sciences and Translational Medicine, University of Rome ‘Tor Vergata’, I‑00133 Rome, Italy, Department of Experimental Medicine, University of Rome ‘Tor Vergata’, I‑00133 Rome, Italy, Department of Experimental Medicine, University of Rome ‘Sapienza’, I‑00161 Rome, Italy
    Copyright: © Benvenuto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2639-2647
    |
    Published online on: August 8, 2019
       https://doi.org/10.3892/etm.2019.7872
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The extracellular matrix (ECM) creates a tissue microenvironment able to regulate cellular signaling. The loss of ECM plasticity is associated with several pathologies, especially those involving chronic inflammation, therefore, the ECM represents a potential therapeutic target for certain conditions. The present study investigated the effects of a natural multi‑component compound formulation, Galium‑Heel®, on the growth, morphology and ECM production of human dermal fibroblasts (HDF). The effects of the formulation on HDF growth and morphology were assessed by sulforhodamine B assay, trypan blue exclusion staining, FACS and ultrastructural analyses. The effect of the compound on reactive oxygen species production by HDF was performed by dichlorofluorescin diacetate assay. The expression of ECM components, matrix metalloproteinases (MMPs) and signaling molecules was analyzed by western blot analysis. The present results demonstrated that Galium‑Heel® did not significantly affect HDF growth, survival, cell cycle or morphology indicating the biocompatibility of the formulation. The formulation demonstrated antioxidant activity. Galium‑Heel® was able to modulate ECM by regulating collagens (type I and III) and MMPs‑3 and ‑7 expression. In addition, the formulation was able to regulate molecules involved in TGF‑β signalling, including mitogen activated kinase‑like protein, GLI family zinc finger 2 and pro‑survival proteins such as AKT. The present results demonstrating the effects of a natural multi‑component compound on ECM composition, highlighted the possibility of pharmacologically modulating ECM molecules. The recovery and the maintenance of ECM homeostasis might be considered as a potential therapeutic goal to ameliorate pathological conditions.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Nyström A and Bruckner-Tuderman L: Matrix molecules and skin biology. Semin Cell Dev Biol. 89:136–146. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Bei R, Masuelli L, Palumbo C, Tresoldi I, Scardino A and Modesti A: Long-lasting tissue inflammatory processes trigger autoimmune responses to extracellular matrix molecules. Int Rev Immunol. 27:137–175. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Iozzo RV and Gubbiotti MA: Extracellular matrix: The driving force of mammalian diseases. Matrix Biol. 71-72:1–9. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Walker C, Mojares E and Del Río Hernández A: Role of extracellular matrix in development and cancer progression. Int J Mol Sci. 19(pii): E30282018. View Article : Google Scholar : PubMed/NCBI

5 

Li L, Zhao Q and Kong W: Extracellular matrix remodeling and cardiac fibrosis. Matrix Biol. 68-69:490–506. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Minihane AM, Vinoy S, Russell WR, Baka A, Roche HM, Tuohy KM, Teeling JL, Blaak EE, Fenech M, Vauzour D, et al: Low-grade inflammation, diet composition and health: Current research evidence and its translation. Br J Nutr. 114:999–1012. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Theocharis AD, Skandalis SS, Gialeli C and Karamanos NK: Extracellular matrix structure. Adv Drug Deliv Rev. 97:4–27. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Soylemezoglu O, Wild G, Dalley AJ, MacNeil S, Milford-Ward A, Brown CB and el Nahas AM: Urinary and serum type III collagen: Markers of renal fibrosis. Nephrol Dial Transplant. 12:1883–1889. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Arpino V, Brock M and Gill SE: The role of TIMPs in regulation of extracellular matrix proteolysis. Matrix Biol. 44-46:247–254. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Randelli F, Menon A, Giai Via A, Mazzoleni MG, Sciancalepore F, Brioschi M and Gagliano N: Effect of a collagen-based compound on morpho-functional properties of cultured human tenocytes. Cells. 7(pii): E2462018. View Article : Google Scholar : PubMed/NCBI

11 

Karsdal MA, Nielsen MJ, Sand JM, Henriksen K, Genovese F, Bay-Jensen AC, Smith V, Adamkewicz JI, Christiansen C and Leeming DJ: Extracellular matrix remodeling: The common denominator in connective tissue diseases. Possibilities for evaluation and current understanding of the matrix as more than a passive architecture, but a key player in tissue failure. Assay Drug Dev Technol. 11:70–92. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Barascuk N, Vassiliadis E, Larsen L, Wang J, Zheng Q, Xing R, Cao Y, Crespo C, Lapret I, Sabatini M, et al: Development and validation of an enzyme-linked immunosorbent assay for the quantification of a specific MMP-9 mediated degradation fragment of type III collagen-A novel biomarker of atherosclerotic plaque remodeling. Clin Biochem. 44:900–906. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Liang Z, Li T, Jiang S, Xu J, Di W, Yang Z, Hu W and Yang Y: AMPK: A novel target for treating hepatic fibrosis. Oncotarget. 8:62780–62792. 2017.PubMed/NCBI

14 

Zhang N, Wei WY, Li LL, Hu C and Tang QZ: Therapeutic potential of polyphenols in cardiac fibrosis. Front Pharmacol. 9:1222018. View Article : Google Scholar : PubMed/NCBI

15 

Katoh M: Multi-layered prevention and treatment of chronic inflammation, organ fibrosis and cancer associated with canonical WNT/β catenin signaling activation (Review). Int J Mol Med. 42:713–725. 2018.PubMed/NCBI

16 

Reale FR, GriYn TW, Compton JM, Graham S, Townes PL and Bogden A: Characterization of a human malignant mesothelioma cell line (H-MESO-1): A biphasic solid and ascitic tumor model. Cancer Res. 47:3199–3205. 1987.PubMed/NCBI

17 

Masuelli L, Benvenuto M, Mattera R, Di Stefano E, Zago E, Taffera G, Tresoldi I, Giganti MG, Frajese GV, Berardi G, et al: In vitro and in vivo anti-tumoral effects of the flavonoid apigenin in malignant mesotelioma. Front Pharmacol. 8:3732017. View Article : Google Scholar : PubMed/NCBI

18 

Strober W: Trypan blue exclusion test of cell viability. Curr Protoc Immunol. 111:A3.B.1–3. 2015. View Article : Google Scholar

19 

Benvenuto M, Mattera R, Sticca JI, Rossi P, Cipriani C, Giganti MG, Volpi A, Modesti A, Masuelli L and Bei R: Effect of the BH3 mimetic polyphenol (−)-Gossypol (AT-101) on the in vitro and in vivo growth of malignant mesothelioma. Front Pharmacol. 9:12692018. View Article : Google Scholar : PubMed/NCBI

20 

Masuelli L, Pantanella F, La Regina G, Benvenuto M, Fantini M, Mattera R, Di Stefano E, Mattei M, Silvestri R, Schippa S, et al: Violacein, an indole-derived purple-colored natural pigment produced by Janthinobacterium lividum, inhibits the growth of head and neck carcinoma cell lines both in vitro and in vivo. Tumour Biol. 37:3705–3717. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Benvenuto M, Mattera R, Masuelli L, Taffera G, Andracchio O, Tresoldi I, Lido P, Giganti MG, Godos J, Modesti A and Bei R: (±)-Gossypol induces apoptosis and autophagy in head and neck carcinoma cell lines and inhibits the growth of transplanted salivary gland cancer cells in BALB/c mice. Int J Food Sci Nutr. 68:298–312. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Masuelli L, Benvenuto M, Di Stefano E, Mattera R, Fantini M, De Feudis G, De Smaele E, Tresoldi I, Giganti MG, Modesti A and Bei R: Curcumin blocks autophagy and activates apoptosis of malignant mesothelioma cell lines and increases the survival of mice intraperitoneally transplanted with a malignant mesothelioma cell line. Oncotarget. 8:34405–34422. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Masuelli L, Granato M, Benvenuto M, Mattera R, Bernardini R, Mattei M, d'Amati G, D'Orazi G, Faggioni A, Bei R and Cirone M: Chloroquine supplementation increases the cytotoxic effect of curcumin against Her2/neu overexpressing breast cancer cells in vitro and in vivo in nude mice while counteracts it in immune competent mice. OncoImmunology. 6:e13561512017. View Article : Google Scholar : PubMed/NCBI

24 

Angelucci C, D'Alessio A, Lama G, Binda E, Mangiola A, Vescovi AL, Proietti G, Masuelli L, Bei R, Fazi B, et al: Cancer stem cells from peritumoral tissue of glioblastoma multiforme: The possible missing link between tumor development and progression. Oncotarget. 9:28116–28130. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Stanković M, Radojević I, Ćurčić M, Vasić S, Topuzović M, Čomić L and Marković S: Evaluation of biological activities of goldmoss stonecrop (Sedum acre L.). Turk J Biol. 36:580–588. 2012.

26 

Sentjurc M, Nemec M, Connor HD and Abram V: Antioxidant activity of Sempervivum tectorum and its components. J Agric Food Chem. 51:2766–2771. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Marrassini C, Acevedo C, Miño J, Ferraro G and Gorzalczany S: Evaluation of antinociceptive, antinflammatory activities and phytochemical analysis of aerial parts of Urtica urens L. Phytother Res. 24:1807–1812. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Lu P, Takai K, Weaver VM and Werb Z: Extracellular matrix degradation and remodeling in development and disease. Cold Spring Harb Perspect Biol. 3(pii): a0050582011.PubMed/NCBI

29 

Sanchavanakit N, Saengtong W, Manokawinchoke J and Pavasant P: TNF-α stimulates MMP-3 production via PGE2 signalling through the NF-kB and p38 MAPK pathway in a murine cementoblast cell line. Arch Oral Biol. 60:1066–1074. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Yang H, Gao F, Li X, Wang J, Liu H and Zheng Z: TGF-β1 antagonizes TNF-α induced up-regulation of matrix metalloproteinase 3 in nucleus pulposus cells: Role of the ERK1/2 pathway. Connect Tissue Res. 56:461–468. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Saed GM, Zhang W, Chegini N, Holmdahl L and Diamond MP: Alteration of type I and III collagen expression in human peritoneal mesothelial cells in response to hypoxia and transforming growth factor-beta1. Wound Repair Regen. 7:504–510. 1999. View Article : Google Scholar : PubMed/NCBI

32 

Forel JM, Guervilly C, Farnarier C, Donati SY, Hraiech S, Persico N, Allardet-Servent J, Coiffard B, Gainnier M, Loundou A, et al: Transforming growth factor-β1 in predicting early lung fibroproliferation in patients with acute respiratory distress syndrome. PLoS One. 13:e02061052018. View Article : Google Scholar : PubMed/NCBI

33 

Rojkind M, Giambrone MA and Biempica L: Collagen types in normal and cirrhotic liver. Gastroenterology. 76:710–719. 1979.PubMed/NCBI

34 

Javelaud D, Pierrat MJ and Mauviel A: Crosstalk between TGF-β and hedgehog signaling in cancer. FEBS Lett. 586:2016–2025. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Liang R, Šumová B, Cordazzo C, Mallano T, Zhang Y, Wohlfahrt T, Dees C, Ramming A, Krasowska D, Michalska-Jakubus M, et al: The transcription factor GLI2 as a downstream mediator of transforming growth factor-β-induced fibroblast activation in SSc. Ann Rheum Dis. 76:756–764. 2017. View Article : Google Scholar : PubMed/NCBI

36 

Moncada S and Higgs A: The L-arginine-nitric oxide pathway. N Engl J Med. 329:2002–2012. 1993. View Article : Google Scholar : PubMed/NCBI

37 

Xia W, Szomor Z, Wang Y and Murrell GA: Nitric oxide enhances collagen synthesis in cultured human tendon cells. J Orthop Res. 24:159–172. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Lundberg JO, Weitzberg E and Gladwin MT: The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nat Rev Drug Discov. 7:156–167. 2008. View Article : Google Scholar : PubMed/NCBI

39 

Gilkes DM, Semenza GL and Wirtz D: Hypoxia and the extracellular matrix: Drivers of tumour metastasis. Nat Rev Cancer. 14:430–439. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Negahdary M, Chelongar R, Zadeh SK and Ajdary M: The antioxidant effects of silver, gold, and zinc oxide nanoparticles on male mice in in vivo condition. Adv Biomed Res. 4:692015. View Article : Google Scholar : PubMed/NCBI

41 

Hashimoto M, Sasaki JI, Yamaguchi S, Kawai K, Kawakami H, Iwasaki Y and Imazato S: Gold nanoparticles inhibit matrix metalloproteases without cytotoxicity. J Dent Res. 94:1085–1091. 2015. View Article : Google Scholar : PubMed/NCBI

42 

Veerendhar A, Reich R and Breuer E: Phosphorus based inhibitors of matrix metalloproteinases. C R Chim. 13:1191–1202. 2010. View Article : Google Scholar

43 

Slompo C, Buzalaf CP, Damante CA, Martins GM, Hannas AR, Buzalaf MA and Oliveira RC: Fluoride modulates preosteoblasts viability and matrix metalloproteinases-2 and −9 activities. Braz Dent J. 23:629–634. 2012. View Article : Google Scholar : PubMed/NCBI

44 

Suszko A and Obmińska-Mrukowicz B: Effects of polysaccharide fractions isolated from Caltha palustris L. on the activity of phagocytic cells & humoral immune response in mice with collagen-induced arthritis: A comparison with methotrexate. Indian J Med Res. 145:229–236. 2017.PubMed/NCBI

45 

Han X and Parker TL: Anti-inflammatory activity of Juniper (Juniperus communis) berry essential oil in human dermal fibroblasts. Cogent Med. 4:13062002017. View Article : Google Scholar

46 

Rastogi S, Pandey MM and Kumar Singh Rawat A: Medicinal plants of the genus Betula-traditional uses and a phytochemical-pharmacological review. J Ethnopharmacol. 159:62–83. 2015. View Article : Google Scholar : PubMed/NCBI

47 

Ra HJ, Lee HJ, Jo HS, Nam DC, Lee YB, Kang BH, Moon DK, Kim DH, Lee CJ and Hwang SC: Betulin suppressed interleukin-1β-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat. Korean J Physiol Pharmacol. 21:19–26. 2017. View Article : Google Scholar : PubMed/NCBI

48 

Silacci P, Dayer JM, Desgeorges A, Peter R, Manueddu C and Guerne PA: Interleukin (IL)-6 and its soluble receptor induce TIMP-1 expression in synoviocytes and chondrocytes, and block IL-1-induced collagenolytic activity. J Biol Chem. 273:13625–13629. 1998. View Article : Google Scholar : PubMed/NCBI

49 

Offergeld R, Reinecker C, Gumz E, Schrum S, Treiber R, Neth RD and Gohla SH: Mitogenic activity of high molecular polysaccharide fractions isolated from the cuppressaceae Thuja occidentalis L. enhanced cytokine-production by thyapolysaccharide, g-fraction (TPSg). Leukemia. 6 (Suppl 3):189S–191S. 1992.PubMed/NCBI

50 

Sunila ES, Hamsa TP and Kuttan G: Effect of Thuja occidentalis and its polysaccharide on cell-mediated immune responses and cytokine levels of metastatic tumor-bearing animals. Pharm Biol. 49:1065–1073. 2011. View Article : Google Scholar : PubMed/NCBI

51 

Masuelli L, Marzocchella L, Focaccetti C, Tresoldi I, Palumbo C, Izzi V, Benvenuto M, Fantini M, Lista F, Tarantino U, et al: Resveratrol and diallyl disulfide enhance curcumin-induced sarcoma cell apoptosis. Front Biosci (Landmark Ed). 17:498–508. 2012. View Article : Google Scholar : PubMed/NCBI

52 

Masuelli L, Di Stefano E, Fantini M, Mattera R, Benvenuto M, Marzocchella L, Sacchetti P, Focaccetti C, Bernardini R, Tresoldi I, et al: Resveratrol potentiates the in vitro and in vivo anti-tumoral effects of curcumin in head and neck carcinomas. Oncotarget. 5:10745–10762. 2014. View Article : Google Scholar : PubMed/NCBI

53 

Fantini M, Benvenuto M, Masuelli L, Frajese GV, Tresoldi I, Modesti A and Bei R: In vitro and in vivo antitumoral effects of combinations of polyphenols, or polyphenols and anticancer drugs: Perspectives on cancer treatment. Int J Mol Sci. 16:9236–9282. 2015. View Article : Google Scholar : PubMed/NCBI

54 

Rhee S: Fibroblasts in three dimensional matrices: Cell migration and matrix remodelling. Exp Mol Med. 41:858–865. 2009. View Article : Google Scholar : PubMed/NCBI

55 

Langevin HM, Bouffard NA, Fox JR, Palmer BM, Wu J, Iatridis JC, Barnes WD, Badger GJ and Howe AK: Fibroblast cytoskeletal remodeling contributes to connective tissue tension. J Cell Physiol. 226:1166–1175. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Benvenuto M, Mattera R, Miele MT, Giganti MG, Tresoldi I, Albonici L, Manzari V, Modesti A, Masuelli L, Bei R, Bei R, et al: Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts. Exp Ther Med 18: 2639-2647, 2019.
APA
Benvenuto, M., Mattera, R., Miele, M.T., Giganti, M.G., Tresoldi, I., Albonici, L. ... Bei, R. (2019). Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts. Experimental and Therapeutic Medicine, 18, 2639-2647. https://doi.org/10.3892/etm.2019.7872
MLA
Benvenuto, M., Mattera, R., Miele, M. T., Giganti, M. G., Tresoldi, I., Albonici, L., Manzari, V., Modesti, A., Masuelli, L., Bei, R."Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts". Experimental and Therapeutic Medicine 18.4 (2019): 2639-2647.
Chicago
Benvenuto, M., Mattera, R., Miele, M. T., Giganti, M. G., Tresoldi, I., Albonici, L., Manzari, V., Modesti, A., Masuelli, L., Bei, R."Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2639-2647. https://doi.org/10.3892/etm.2019.7872
Copy and paste a formatted citation
x
Spandidos Publications style
Benvenuto M, Mattera R, Miele MT, Giganti MG, Tresoldi I, Albonici L, Manzari V, Modesti A, Masuelli L, Bei R, Bei R, et al: Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts. Exp Ther Med 18: 2639-2647, 2019.
APA
Benvenuto, M., Mattera, R., Miele, M.T., Giganti, M.G., Tresoldi, I., Albonici, L. ... Bei, R. (2019). Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts. Experimental and Therapeutic Medicine, 18, 2639-2647. https://doi.org/10.3892/etm.2019.7872
MLA
Benvenuto, M., Mattera, R., Miele, M. T., Giganti, M. G., Tresoldi, I., Albonici, L., Manzari, V., Modesti, A., Masuelli, L., Bei, R."Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts". Experimental and Therapeutic Medicine 18.4 (2019): 2639-2647.
Chicago
Benvenuto, M., Mattera, R., Miele, M. T., Giganti, M. G., Tresoldi, I., Albonici, L., Manzari, V., Modesti, A., Masuelli, L., Bei, R."Effects of a natural multi‑component compound formulation on the growth, morphology and extracellular matrix production of human adult dermal fibroblasts". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2639-2647. https://doi.org/10.3892/etm.2019.7872
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team