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Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis

  • Authors:
    • Hui Zhang
    • Jingbin Yu
    • Hu Sun
    • Yunhe Zhao
    • Jitao Wang
    • Juan Zhang
    • Bin Meng
  • View Affiliations / Copyright

    Affiliations: Intracardiac Department, Central Hospital of Zibo, Zibo, Shandong 255000, P.R. China, Department of Neurosurgery, Central Hospital of Zibo, Zibo, Shandong 255000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2799-2804
    |
    Published online on: August 14, 2019
       https://doi.org/10.3892/etm.2019.7895
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Abstract

Effects of ubiquitin-proteasome system (UPS) inhibitor MG-132 on the expression levels of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in mice with viral myocarditis were investigated to analyze the correlation of myocardial tissue score of mice between TNF-α and TGF-β1. Eighty healthy male SPF mice aged 6 weeks were selected and 20 mice were randomly selected as the blank group. The blank group did not receive any intervention. Mortality rates of each group were recorded and compared on day 8 of modeling, and heart specimens from the remaining mice were histopathologically examined and the expression of mRNA and protein of TNF-α and TGF-β1 in myocardial tissues were detected by western blot analysis. Correlation between mouse myocardial histopathologic scores and expression of protein of TNF-α and TGF-β1 in myocardial tissues, as well as the expression of TNF-α and TGF-β1 in myocardial tissue in VMC mice was analyzed. The expression levels of myocardial histopathological scores, mRNA and protein of TNF-α and TGF-β1 in the blank and control group were significantly lower than those in the VMC and the MG-132 group. The myocardial histopathological scores, mRNA and TNF-α and TGF-β1 protein in the MG-132 group were significantly lower than those in the VMC group (P<0.05). The expression of TNF-α and TGF-β1 protein in myocardial tissues was positively correlated with the pathological score in myocardial tissue of mice (r=0.843, P<0.05; r=0.763, P<0.05), and there was a positive correlation between the expression of TNF-α and TGF-β1 protein in myocardial tissues of VMC mice (r=0.672, P<0.05). UPS inhibitor MG-132, which can significantly alleviate the myocardial injury of VMC mice, reduced the expression of inflammatory factors in myocardial tissues, and improved the survival rate of mice, thus it is a potential new treatment for VMC.
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1 

Pal VK, Bandyopadhyay P and Singh A: Hydrogen sulfide in physiology and pathogenesis of bacteria and viruses. IUBMB Life. 70:393–410. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Guo YB, Zhou LW, Chen FL and Zou N: Effects and related mechanism of overexpression of human thioredoxin on the inflammatory response in mice with viral myocarditis. Zhonghua Xin Xue Guan Bing Za Zhi. 46:444–449. 2018.(In Chinese). PubMed/NCBI

3 

Cao SS, Luo KL and Shi L: Endoplasmic reticulum stress interacts with inflammation in human diseases. J Cell Physiol. 231:288–294. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Huang Z, Rose AH and Hoffmann PR: The role of selenium in inflammation and immunity: From molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal. 16:705–743. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Crawford LJ and Irvine AE: Targeting the ubiquitin proteasome system in haematological malignancies. Blood Rev. 27:297–304. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Ao N, Chen Q and Liu G: The small molecules targeting ubiquitin-proteasome system for cancer therapy. Comb Chem High Throughput Screen. 20:403–413. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Schlossarek S, Frey N and Carrier L: Ubiquitin-proteasome system and hereditary cardiomyopathies. J Mol Cell Cardiol. 71:25–31. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Treuer AV and Gonzalez DR: Nitric oxide synthases, S-nitrosylation and cardiovascular health: From molecular mechanisms to therapeutic opportunities (Review). Mol Med Rep. 11:1555–1565. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Kasaikina MV, Hatfield DL and Gladyshev VN: Understanding selenoprotein function and regulation through the use of rodent models. Biochim Biophys Acta. 1823:1633–1642. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Radwan M, Wilkinson DJ, Hui W, Destrument AP, Charlton SH, Barter MJ, Gibson B, Coulombe J, Gray DA, Rowan AD, et al: Protection against murine osteoarthritis by inhibition of the 26S proteasome and lysine-48 linked ubiquitination. Ann Rheum Dis. 74:1580–1587. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Sun XH, Fu J and Sun DQ: Halofuginone alleviates acute viral myocarditis in suckling BALB/c mice by inhibiting TGF-β1. Biochem Biophys Res Commun. 473:558–564. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Reisz JA, Barrett AS, Nemkov T, Hansen KC and D'Alessandro A: When nature's robots go rogue: Exploring protein homeostasis dysfunction and the implications for understanding human aging disease pathologies. Expert Rev Proteomics. 15:293–309. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Stein EA, Pinkert S, Becher PM, Geisler A, Zeichhardt H, Klopfleisch R, Poller W, Tschöpe C, Lassner D, Fechner H, et al: Combination of RNA interference and virus receptor trap exerts additive antiviral activity in coxsackievirus B3-induced myocarditis in mice. J Infect Dis. 211:613–622. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Maiese K, Hou J, Chong ZZ and Shang YC: A fork in the path: Developing therapeutic inroads with FoxO proteins. Oxid Med Cell Longev. 2:119–129. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Maiese K, Chong ZZ, Hou J and Shang YC: The ‘O’ class: Crafting clinical care with FoxO transcription factors. Adv Exp Med Biol. 665:242–260. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Maiese K, Chong ZZ, Shang YC and Hou J: Clever cancer strategies with FoxO transcription factors. Cell Cycle. 7:3829–3839. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Teale A, Campbell S, Van Buuren N, Magee WC, Watmough K, Couturier B, Shipclark R and Barry M: Orthopoxviruses require a functional ubiquitin-proteasome system for productive replication. J Virol. 83:2099–2108. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Zhang H, Diab A, Fan H, Mani SK, Hullinger R, Merle P and Andrisani O: PLK1 and HOTAIR accelerate proteasomal degradation of SUZ12 and ZNF198 during hepatitis B virus-induced liver carcinogenesis. Cancer Res. 75:2363–2374. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Lee H, Park J, Kim EE, Yoo YS and Song EJ: Proteasome inhibitors attenuated cholesterol-induced cardiac hypertrophy in H9c2 cells. BMB Rep. 49:270–275. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Maiese K, Chong ZZ, Shang YC and Hou J: FoxO proteins: Cunning concepts and considerations for the cardiovascular system. Clin Sci (Lond). 116:191–203. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Magesh S, Chen Y and Hu L: Small molecule modulators of Keap1-Nrf2-ARE pathway as potential preventive and therapeutic agents. Med Res Rev. 32:687–726. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Sionov RV, Vlahopoulos SA and Granot Z: Regulation of Bim in health and disease. Oncotarget. 6:23058–23134. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Pagan J, Seto T, Pagano M and Cittadini A: Role of the ubiquitin proteasome system in the heart. Circ Res. 112:1046–1058. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Yu J, Sun H, Zhao Y, Wang J, Zhang J and Meng B: Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis. Exp Ther Med 18: 2799-2804, 2019.
APA
Zhang, H., Yu, J., Sun, H., Zhao, Y., Wang, J., Zhang, J., & Meng, B. (2019). Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis. Experimental and Therapeutic Medicine, 18, 2799-2804. https://doi.org/10.3892/etm.2019.7895
MLA
Zhang, H., Yu, J., Sun, H., Zhao, Y., Wang, J., Zhang, J., Meng, B."Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis". Experimental and Therapeutic Medicine 18.4 (2019): 2799-2804.
Chicago
Zhang, H., Yu, J., Sun, H., Zhao, Y., Wang, J., Zhang, J., Meng, B."Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2799-2804. https://doi.org/10.3892/etm.2019.7895
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Yu J, Sun H, Zhao Y, Wang J, Zhang J and Meng B: Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis. Exp Ther Med 18: 2799-2804, 2019.
APA
Zhang, H., Yu, J., Sun, H., Zhao, Y., Wang, J., Zhang, J., & Meng, B. (2019). Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis. Experimental and Therapeutic Medicine, 18, 2799-2804. https://doi.org/10.3892/etm.2019.7895
MLA
Zhang, H., Yu, J., Sun, H., Zhao, Y., Wang, J., Zhang, J., Meng, B."Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis". Experimental and Therapeutic Medicine 18.4 (2019): 2799-2804.
Chicago
Zhang, H., Yu, J., Sun, H., Zhao, Y., Wang, J., Zhang, J., Meng, B."Effects of ubiquitin-proteasome inhibitor on the expression levels of TNF-α and TGF-β1 in mice with viral myocarditis". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2799-2804. https://doi.org/10.3892/etm.2019.7895
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