Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2019 Volume 18 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2019 Volume 18 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells

  • Authors:
    • Jing Lu
    • Zhuoqun Meng
    • Bijun Cheng
    • Meitong Liu
    • Siyu Tao
    • Shuang Guan
  • View Affiliations / Copyright

    Affiliations: Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, Jilin 130062, P.R. China, Department of Pharmacology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2965-2971
    |
    Published online on: August 16, 2019
       https://doi.org/10.3892/etm.2019.7905
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In recent years, increasing attention has been paid to diseases caused by excessive accumulation of lipids in the liver with therapeutic agents derived from natural products offering an alternative treatment to conventional therapies. Among these therapeutic agents, apigenin, a natural flavonoid, has been proven to exert various beneficial biological effects. In the present study, the antiadipogenic effects of apigenin in HepG2 cells was investigated. It was demonstrated that the treatment of cells with different concentrations of apigenin for 24 h significantly decreased the palmitic acid‑induced increases in total cholesterol (TC) and triglyceride (TG) levels as well as intracellular lipid accumulation. In addition, apigenin increased the phosphorylated‑AMP‑activated protein kinase (AMPK) levels but decreased the expression levels of 3‑hydroxy‑3‑methylglutaryl CoA reductase, sterol regulatory element‑binding protein (SREBP)‑1, fatty acid synthase, and SREBP‑2 in a concentration‑dependent manner. The present findings suggested that apigenin might improve lipid metabolism by activating the AMPK/SREBP pathway to reduce lipid accumulation in the liver.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Williams JA, Manley S and Ding WX: New advances in molecular mechanisms and emerging therapeutic targets in alcoholic liver diseases. World J Gastroenterol. 20:12908–12933. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Wan Y, Liu LY, Hong ZF and Peng J: Ethanol extract of Cirsium japonicum attenuates hepatic lipid accumulation via AMPK activation in human HepG2 cells. Exp Ther Med. 8:79–84. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Zhang M, Yuan Y, Wang Q, Li X, Men J and Lin M: The Chinese medicine Chai Hu Li Zhong Tang protects against non-alcoholic fatty liver disease by activating AMPKα. Biosci Rep. 38:BSR201806442018. View Article : Google Scholar : PubMed/NCBI

4 

Ono M and Fujimori K: Antiadipogenic effect of dietary apigenin through activation of AMPK in 3T3-L1 cells. J Agric Food Chem. 59:13346–13352. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Xiao X and Song BL: SREBP: A novel therapeutic target. Acta Biochim Biophys Sin. 45:2–10. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Shimano H: Sterol regulatory element binding proteins (SREBPs): Transcriptional regulators of lipid synthetic genes. Prog Lipid Res. 40:439–452. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Wong TY, Lin SM and Leung LK: The flavone luteolin suppresses SREBP-2 expression and post-translational activation in hepatic cells. PLoS One. 10:e01356372015. View Article : Google Scholar : PubMed/NCBI

8 

Mottillo EP, Desjardins EM, Crane JD, Smith BK, Green AE, Ducommun S, Henriksen TI, Rebalka IA, Razi A, Sakamoto K, et al: Lack of adipocyte AMPK exacerbates insulin resistance and hepatic steatosis through brown and beige adipose tissue function. Cell Metab. 24:118–129. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Lu J, Cheng B, Meng Z, Sun M, Fang B, Li T, Sun M, Liu M and Guan S: Alliin attenuates 1,3-dichloro-2-propanol-induced lipogenesis in HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway. Life Sci. 195:19–24. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Lordan S, O'Neill C and O'Brien NM: Effects of apigenin, lycopene and astaxanthin on 7 beta-hydroxycholesterol-induced apoptosis and Akt phosphorylation in U937 cells. Br J Nutr. 100:287–296. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Tong X and Pelling J: Targeting the PI3K/Akt/mTOR axis by apigenin for cancer prevention. Anticancer Agents Med Chem. 13:971–978. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Zhu ZY, Gao T, Huang Y, Xue J and Xie ML: Apigenin ameliorates hypertension-induced cardiac hypertrophy and down-regulates cardiac hypoxia inducible factor-lα in rats. Food Funct. 7:1992–1998. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Ren B, Qin W, Wu F, Wang S, Pan C, Wang L, Zeng B, Ma S and Liang J: Apigenin and naringenin regulate glucose and lipid metabolism, and ameliorate vascular dysfunction in type 2 diabetic rats. Eur J Pharmacol. 773:13–23. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Escande C, Nin V, Price NL, Capellini V, Gomes AP, Barbosa MT, O'Neil L, White TA, Sinclair DA and Chini EN: Flavonoid apigenin is an inhibitor of the NAD+ ase CD38: Implications for cellular NAD+ metabolism, protein acetylation, and treatment of metabolic syndrome. Diabetes. 62:1084–1093. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Poitout V and Robertson RP: Glucolipotoxicity: Fuel excess and beta-cell dysfunction. Endocr Rev. 29:351–366. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Mayer CM and Belsham DD: Palmitate acid attenuates insulin signaling and induces endoplasmic reticulum stress and apoptosis in hypothalamic neurons: The rescue of resistance and apoptosis through adenosine 5′ monophosphate-activated protein kinase activation. Endocrinology. 151:576–585. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Park JY, Kim Y, Im JA and Lee H: Oligonol suppresses lipid accumulation and improves insulin resistance in a palmitate-induced in HepG2 hepatocytes as a cellular steatosis model. BMC Complement Altern Med. 15:1852015. View Article : Google Scholar : PubMed/NCBI

18 

Kim J, Lee I, Seo J, Jung M, Kim Y, Yim N and Bae K: Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3-L1 cells. Phytother Res. 24:1543–1548. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Ren K, Jiang T, Zhou HF, Liang Y and Zhao GJ: Apigenin retards atherogenesis by promoting ABCA1-mediated cholesterol efflux and suppressing inflammation. Cell Physiol Biochem. 47:2170–2184. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Vock C, Gleissner M, Klapper M and Döring F: Identification of palmitate-regulated genes in HepG2 cells by applying microarray analysis. Biochim Biophys Acta. 1770:1283–1288. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Zechner R, Zimmermann R, Eichmann TO, Kohlwein SD, Haemmerle G, Lass A and Madeo F: FAT SIGNALS-lipases and lipolysis in lipid metabolism and signaling. Cell Metab. 15:279–291. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Block MJ: American chemical society. Chemcycolpedia. 2000:106–107. 2001.

23 

Zhang J, Liu D, Huang Y, Gao Y and Qian S: Biopharmaceutics classification and intestinal absorption study of apigenin. Int J Pharm. 436:311–317. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Altamimi MA, Elzayat EM, Alshehri SM, Mohsin K, Ibrahim MA, Al Meanazel OT, Shakeel F, Alanazi FK and Alsarra IA: Utilizing spray drying technique to improve oral bioavailability of apigenin. Adv Powder Technol. 29:1676–1684. 2018. View Article : Google Scholar

25 

Zhai YJ, Guo SS, Liu CH, Yang CF, Dou JF, Li LB and Zhai GX: Preparation and in vitro evaluation of apigenin-loaded polymeric micelles. Colloids Surfaces A: Physicochemical Engineering Aspects. 429:24–30. 2013. View Article : Google Scholar

26 

Hardie DG: Minireview: The AMP-activated protein kinase cascade: The key sensor of cellular energy status. Endocrinology. 144:5179–5183. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Carling D: The AMP-activated protein kinase cascade-a unifying system for energy control. Trends Biochem Sci. 29:18–24. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Kang MC, Ding Y, Kim EA, Choi YK, de Araujo T, Heo SJ and Lee SH: Indole derivatives isolated from Brown alga Sargassum thunbergii inhibit Adipogenesis through AMPK activation in 3T3-L1 preadipocytes. Mar Drugs. 15:E1192017. View Article : Google Scholar : PubMed/NCBI

29 

Long YC and Zierath JR: AMP-activated protein kinase signaling in metabolic regulation. J Clin Invest. 116:1776–1783. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Ruderman N and Prentki M: AMP kinase and malonyl-CoA: Targets for therapy of the metabolic syndrome. Nat Rev Drug Discov. 3:340–351. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Sun X, Duan X, Wang C, Liu Z, Sun P, Huo X, Ma X, Sun H, Liu K and Meng Q: Protective effects of glycyrrhizic acid against non-alcoholic fatty liver disease in mice. Eur J Pharmacol. 5:75–82. 2017. View Article : Google Scholar

32 

Frederico MJ, Vitto MF, Cesconetto PA, Engelmann J, De Souza DR, Luz G, Pinho RA, Ropelle ER, Cintra DE and De Souza CT: Short-term inbibition of SREBP-1c expression reversed dietinduced nonalcoholic fatty liver disease in mice. Scand J Gastroenterol. 46:1381–1388. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Sakakura Y, Shimano H, Sone H, Takahashi A, Inoue N, Toyoshima H, Suzuki S and Yamada N: Sterol regulatory element-binding proteins induce an entire pathway of cholesterol synthesis. Biochem Biophys Res Commun. 286:176–183. 2001. View Article : Google Scholar : PubMed/NCBI

34 

Caballero F, Fernandez A, De-Lacy AM, Fernandez-Checa JC, Caballeria J and Garcia-Ruiz C: Enhanced free cholesterol, SREBP-2 and StAR expression in human NASH. J Hepatol. 50:789–796. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Sun JH, Liu X, Cong LX, Li H, Zhang CY, Chen JG and Wang CM: Metabolomics study of therapeutic mechanism of Schisandra Chinensislignans in diet-induced hyperlipidemia mice. Lipids Health Dis. 16:1452017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lu J, Meng Z, Cheng B, Liu M, Tao S and Guan S: Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells. Exp Ther Med 18: 2965-2971, 2019.
APA
Lu, J., Meng, Z., Cheng, B., Liu, M., Tao, S., & Guan, S. (2019). Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells. Experimental and Therapeutic Medicine, 18, 2965-2971. https://doi.org/10.3892/etm.2019.7905
MLA
Lu, J., Meng, Z., Cheng, B., Liu, M., Tao, S., Guan, S."Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells". Experimental and Therapeutic Medicine 18.4 (2019): 2965-2971.
Chicago
Lu, J., Meng, Z., Cheng, B., Liu, M., Tao, S., Guan, S."Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2965-2971. https://doi.org/10.3892/etm.2019.7905
Copy and paste a formatted citation
x
Spandidos Publications style
Lu J, Meng Z, Cheng B, Liu M, Tao S and Guan S: Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells. Exp Ther Med 18: 2965-2971, 2019.
APA
Lu, J., Meng, Z., Cheng, B., Liu, M., Tao, S., & Guan, S. (2019). Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells. Experimental and Therapeutic Medicine, 18, 2965-2971. https://doi.org/10.3892/etm.2019.7905
MLA
Lu, J., Meng, Z., Cheng, B., Liu, M., Tao, S., Guan, S."Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells". Experimental and Therapeutic Medicine 18.4 (2019): 2965-2971.
Chicago
Lu, J., Meng, Z., Cheng, B., Liu, M., Tao, S., Guan, S."Apigenin reduces the excessive accumulation of lipids induced by palmitic acid via the AMPK signaling pathway in HepG2 cells". Experimental and Therapeutic Medicine 18, no. 4 (2019): 2965-2971. https://doi.org/10.3892/etm.2019.7905
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team