Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
November-2019 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2019 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells

  • Authors:
    • Yi Liu
    • Adili Salai
    • Dan He
    • Shengli Gao
    • Wei Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Shenzhen University General Hospital, Shenzhen, Guangdong 518055, P.R. China, Department of Thoracic Surgery, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China, Department of Hematology and Oncology, Shenzhen University General Hospital, Shenzhen, Guangdong 518055, P.R. China
  • Pages: 3622-3630
    |
    Published online on: August 26, 2019
       https://doi.org/10.3892/etm.2019.7940
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

MicroRNA (miR)‑10b is highly expressed in esophageal cancer tissues and is associated with poor prognosis of esophageal cancer. However, the role and mechanism of miR‑10b in esophageal cancer cells remains unclear, therefore, the present study aimed to investigate this. Esophageal cancer cells, TE‑1 and EC9706, were transfected with miR‑10b mimic, miR‑10b inhibitor or incubated with transforming growth factor‑β (TGF‑β). MTT and EdU assays were used to detect cell proliferation. Flow cytometry was used to determine cell cycle analysis and apoptosis. Cell migration and invasion were also analyzed. Western blot analysis was used to detect protein levels and reverse transcription‑quantitative PCR was used to analyze miR‑10b expression. The present results demonstrated that, compared with the control group, miR‑10b significantly promoted TE‑1 and EC9706 cell proliferation. Compared with miR‑10b inhibitor group and control group, miR‑10b mimic promoted esophageal cancer cell cycle progression, inhibited apoptosis of esophageal cancer cells and promoted the migration and invasion of cells. The proliferation of esophageal cancer cells increased in a dose‑dependent manner with TGF‑β concentration. TGF‑β treatment induced high expression of miR‑10b in both cell lines. The miR‑10b mimic + TGF‑β group further promoted the migration and invasion of esophageal cancer cells. Western blot analysis determined that, compared with the control group, miR‑10b mimic increased TGF‑β expression. miR‑10b mimic also inhibited the expression of phosphatase and tensin homolog (PTEN) in tumor cells. Compared with the control group, TGF‑β inhibited the expression of PTEN with the miR‑10b mimic + TGF‑β group further inhibiting the PTEN. miR‑10b inhibitor + TGF‑β reversed the effect of TGF‑β and miR‑10b on PTEN. In conclusion, miR‑10b promoted cell cycle progression, inhibited apoptosis and promoted the migration and invasion of esophageal cancer cells. The mechanism may be related to the upregulation of TGF‑β and the downregulation of PTEN. The present findings suggested that miR‑10b might be a potential therapeutic target for esophageal cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Stappert L, Roese-Koerner B and Brustle O: The role of microRNAs in human neural stem cells, neuronal differentiation and subtype specification. Cell Tissue Res. 359:47–64. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Villegas-Ruiz V, Juárez-Méndez S, Pérez-González OA, Arreola H, Paniagua-García L, Parra-Melquiadez M, Peralta-Rodríguez R, López-Romero R, Monroy-García A, Mantilla-Morales A, et al: Heterogeneity of microRNAs expression in cervical cancer cells: Over-expression of miR-196a. Int J Clin Exp Pathol. 7:1389–1401. 2014.PubMed/NCBI

3 

Ye JJ and Cao J: MicroRNAs in colorectal cancer as markers and targets: Recent advances. World J Gastroenterol. 20:4288–4299. 2014. View Article : Google Scholar : PubMed/NCBI

4 

He B, Yin B, Wang B, Xia Z, Chen C and Tang J: MicroRNAs in esophageal cancer (review). Mol Med Rep. 6:459–465. 2012.PubMed/NCBI

5 

Ahmad A, Ginnebaugh KR, Yin S, Bollig-Fischer A, Reddy KB and Sarkar FH: Functional role of miR-10b in tamoxifen resistance of ER-positive breast cancer cells through down-regulation of HDAC4. BMC Cancer. 15:5402015. View Article : Google Scholar : PubMed/NCBI

6 

Knirsh R, Ben-Dror I, Modai S, Shomron N and Vardimon L: MicroRNA 10b promotes abnormal expression of the proto-oncogene c-Jun in metastatic breast cancer cells. Oncotarget. 7:59932–59944. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Zhen L, Li J, Zhang M and Yang K: MiR-10b decreases sensitivity of glioblastoma cells to radiation by targeting AKT. J Biol Res (Thessalon). 23:142016. View Article : Google Scholar : PubMed/NCBI

8 

Ma L, Teruya-Feldstein J and Weinberg RA: Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 449:682–688. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Short MW, Burgers KG and Fry VT: Esophageal cancer. Am Fam Physician. 95:22–28. 2017.PubMed/NCBI

10 

Lin Y, Totsuka Y, Shan B, Wang C, Wei W, Qiao Y, Kikuchi S, Inoue M, Tanaka H and He Y: Esophageal cancer in high-risk areas of China: Research progress and challenges. Ann Epidemiol. 27:215–221. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Xu H, Yao Y, Meng F, Qian X, Jiang X, Li X, Gao Z and Gao L: Predictive value of serum miR-10b, miR-29c, and miR-205 as promising biomarkers in esophageal squamous cell carcinoma screening. Medicine (Baltimore). 94:e15582015. View Article : Google Scholar : PubMed/NCBI

12 

Massagué J: TGFbeta in cancer. Cell. 134:215–230. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Mishra L, Derynck R and Mishra B: Transforming growth factor-beta signaling in stem cells and cancer. Science. 310:68–71. 2005. View Article : Google Scholar : PubMed/NCBI

14 

Madhunapantula SV and Robertson GP: The PTEN-AKT3 signaling cascade as a therapeutic target in melanoma. Pigment Cell Melanoma Res. 22:400–419. 2010. View Article : Google Scholar

15 

Georgescu MM: PTEN tumor suppressor Network in PI3K-Akt pathway control. Genes Cancer. 1:1170–1177. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Jamali L, Tofigh R, Tutunchi S, Panahi G, Borhani F, Akhavan S, Nourmohammadi P, Ghaderian SMH, Rasouli M and Mirzaei H: Circulating microRNAs as diagnostic and therapeutic biomarkers in gastric and esophageal cancers. J Cell Physiol. 233:8538–8550. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods Dec. 25:402–408. 2001. View Article : Google Scholar

18 

Xu W, Yang Z, Zhou SF and Lu N: Posttranslational regulation of phosphatase and tensin homolog (PTEN) and its functional impact on cancer behaviors. Drug Des Devel Ther. 8:1745–1751. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Bahena-Ocampo I, Espinosa M, Ceballos-Cancino G, Lizarraga F, Campos-Arroyo D, Schwarz A, Garcia-Lopez P, Maldonado V and Melendez-Zajgla J: miR-10b expression in breast cancer stem cells supports self-renewal through negative PTEN regulation and sustained AKT activation. EMBO Rep. 17:10812016. View Article : Google Scholar : PubMed/NCBI

20 

Wang W: Study of miR-10b regulatory mechanism for epithelial-mesenchymal transition, invasion and migration in nasopharyngeal carcinoma cells. Oncol Lett. 14:7207–7210. 2017.PubMed/NCBI

21 

Zhang Y, Liao RB, Hu LL, Tong BX, Hao TF and Wu HJ: The microRNA miR-10b as a potentially promising biomarker to predict the prognosis of cancer patients: A meta-analysis. Oncotarget. 8:104543–104551. 2017.PubMed/NCBI

22 

Tian Y, Luo A, Cai Y, Su Q, Ding F, Chen H and Liu Z: MicroRNA-10b promotes migration and invasion through KLF4 in human esophageal cancer cell lines. J Biol Chem. 285:7986–7994. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Carlsson J, Christiansen J, Davidsson S, Giunchi F, Fiorentino M and Sundqvist P: The potential role of miR-126, miR-21 and miR-10b as prognostic biomarkers in renal cell carcinoma. Oncol Lett. 17:4566–4574. 2019.PubMed/NCBI

24 

Zhang J, Yang J, Zhang X, Xu J, Sun Y and Zhang P: MicroRNA-10b expression in breast cancer and its clinical association. PLoS One. 13:e01925092018. View Article : Google Scholar : PubMed/NCBI

25 

Hujie G, Zhou SH, Zhang H, Qu J, Xiong XW, Hujie O, Liao CG and Yang SE: MicroRNA-10b regulates epithelial-mesenchymal transition by modulating KLF4/KLF11/Smads in hepatocellular carcinoma. Cancer Cell Int. 18:102018. View Article : Google Scholar : PubMed/NCBI

26 

Moustakas A and Heldin CH: The regulation of TGFbeta signal transduction. Development. 136:3699–3714. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Pickup M, Novitskiy S and Moses HL: The roles of TGF beta in the tumour microenvironment. Nat Rev Cancer. 13:788–799. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Zheng T and Yang J: Mechanism of transforming growth factor β in patients with hepatocellular carcinoma. Chin J Hepatobiliary Surg. 6:425–428. 2016.

29 

He Z, Dong W, Li Q, Qin C and Li Y: Sauchinone prevents TGF-β-induced EMT and metastasis in gastric cancer cells. Biomed Pharmacother. 101:355–361. 2018. View Article : Google Scholar : PubMed/NCBI

30 

Katsuno Y, Lamouille S and Derynck R: TGF-beta signaling and epithelial-mesenchymal transition in cancer progression. Curr Opin Oncol. 25:76–84. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Ma C, Wei F, Xia H, Liu H, Dong X, Zhang Y, Luo Q, Liu Y and Li Y: MicroRNA-10b mediates TGF-β1-regulated glioblastoma proliferation, migration and epithelial-mesenchymal transition. Int J Oncol. 50:1739–1748. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Ouyang H, Gore J, Deitz S and Korc M: MicroRNA-10b enhances pancreatic cancer cell invasion by suppressing TIP30 expression and promoting EGF and TGF-beta actions. Oncogene. 36:49522017. View Article : Google Scholar : PubMed/NCBI

33 

Zhong H, Wang HR, Yang S, Zhong JH, Wang T, Wang C and Chen FY: Targeting Smad4 links microRNA-146a to the TGF-beta pathway during retinoid acid induction in acute promyelocytic leukemia cell line. Int J Hematol. 92:129–135. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Liu MX, Liao J, Xie M, Gao ZK, Wang XH, Zhang Y, Shang MH, Yin LH, Pu YP and Liu R: miR-93-5p transferred by exosomes promotes the proliferation of esophageal cancer cells via intercellular communication by targeting PTEN. Biomed Environ Sci. 31:171–185. 2018.PubMed/NCBI

35 

Liang HX, Sun LB and Liu NJ: Neferine inhibits proliferation, migration and invasion of U251 glioma cells by down-regulation of miR-10b. Biomed Pharmacother. 109:1032–1040. 2019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Salai A, He D, Gao S and Zeng W: Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells . Exp Ther Med 18: 3622-3630, 2019.
APA
Liu, Y., Salai, A., He, D., Gao, S., & Zeng, W. (2019). Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells . Experimental and Therapeutic Medicine, 18, 3622-3630. https://doi.org/10.3892/etm.2019.7940
MLA
Liu, Y., Salai, A., He, D., Gao, S., Zeng, W."Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells ". Experimental and Therapeutic Medicine 18.5 (2019): 3622-3630.
Chicago
Liu, Y., Salai, A., He, D., Gao, S., Zeng, W."Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells ". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3622-3630. https://doi.org/10.3892/etm.2019.7940
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Salai A, He D, Gao S and Zeng W: Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells . Exp Ther Med 18: 3622-3630, 2019.
APA
Liu, Y., Salai, A., He, D., Gao, S., & Zeng, W. (2019). Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells . Experimental and Therapeutic Medicine, 18, 3622-3630. https://doi.org/10.3892/etm.2019.7940
MLA
Liu, Y., Salai, A., He, D., Gao, S., Zeng, W."Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells ". Experimental and Therapeutic Medicine 18.5 (2019): 3622-3630.
Chicago
Liu, Y., Salai, A., He, D., Gao, S., Zeng, W."Effect and mechanism of microRNA‑10b on proliferation and invasion of esophageal cancer cells ". Experimental and Therapeutic Medicine 18, no. 5 (2019): 3622-3630. https://doi.org/10.3892/etm.2019.7940
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team