Homocysteine inhibits the viability and migration ability of human umbilical vein endothelial cells by downregulating the expression of vascular endothelial growth factor

Yu,Jian; Zhang,Ling‑Ling; Wu,Xiao‑Peng; Zhao,Rui; Meng,Zhi‑Xin; Wang,Kun; Wang,Bin; Wang,Hui; Shi,Zuo‑Lei; Li,Guang‑Xin

doi:10.3892/etm.2019.8043

November-2019 Volume 18 Issue 5

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November-2019 Volume 18 Issue 5

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Article Open Access

Homocysteine inhibits the viability and migration ability of human umbilical vein endothelial cells by downregulating the expression of vascular endothelial growth factor

Authors:
- Jian Yu
- Ling‑Ling Zhang
- Xiao‑Peng Wu
- Rui Zhao
- Zhi‑Xin Meng
- Kun Wang
- Bin Wang
- Hui Wang
- Zuo‑Lei Shi
- Guang‑Xin Li
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Affiliations: Department of Vascular Surgery, The First Affiliated Hospital of Shandong First Medical University, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China, Department of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China

Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 3913-3919
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Published online on: September 25, 2019

https://doi.org/10.3892/etm.2019.8043
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Abstract

The current study aimed to explore the effect of homocysteine (Hcy) on the viability and migration ability of human umbilical vein endothelial cells (HUVECs), as well as to examine the underlying mechanism. The association between the expression level of Hcy and lower extremity deep vein thrombosis (DVT) was detected in clinical samples collected from patients. In addition, the effect of Hcy on the viability and migration ability of HUVECs was detected by cell counting kit‑8 and Transwell assays, respectively, while vascular endothelial growth factor (VEGF) expression was measured in order to verify the effect of Hcy on VEGF. The results indicated that the serum Hcy levels in DVT patients were significantly increased. In vitro experiments also confirmed that Hcy was able to significantly inhibit the viability and migration ability of HUVECs, and downregulate the expression of VEGF in these cells. Furthermore, the inhibitory effect of Hcy on HUVEC viability and migration ability was achieved by downregulating the expression of VEGF using small interfering RNA transfection. In conclusion, Hcy inhibited the viability and migration ability of HUVECs by downregulating the expression of VEGF. This may underlie the high incidence of DVT in patients with hyperhomocysteinemia.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Homocysteine inhibits the viability and migration ability of human umbilical vein endothelial cells by downregulating the expression of vascular endothelial growth factor

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