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Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms

  • Authors:
    • Yaqian Zhang
    • Yulin Zhao
    • Dong Dong
    • Xiaoping Li
    • Zhi Li
    • Siyu Li
    • Juan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Rhinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1267-1274
    |
    Published online on: December 18, 2019
       https://doi.org/10.3892/etm.2019.8344
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Abstract

Staphylococcus aureus (S. aureus) is associated with recalcitrant chronic infection, especially in chronic rhinosinusitis (CRS). S. aureus infection and biofilms cause poorer postsurgical outcomes. We developed isosorbide mononitrate (ISMN) loaded nanoparticles conjugated with an anti‑Staphylococcus aureus alpha‑toxin (anti‑S. aureus α-toxin) antibody that could target biofilms and investigated their anti‑biofilm effect. Anti‑S. aureus α-toxin antibody coupled immunoliposomes were generated. The effect of ISMN immunoliposomes on S. aureus biofilm formation and their anti‑biofilm efficacy were examined using the crystal violet method and confocal laser scanning microscopy, respectively. Relative biofilm viability at 24 h was tested using the alamarBlue assay. The biofilm formation inhibitory effect on all concentrations of ISMN immunoliposomes was stronger than that of ISMN liposomes and free ISMN (P<0.05). At concentrations of 45 and 23 mg/ml, the inhibitory effect of ISMN liposomes was stronger than that of free ISMN (P<0.05), while at 11 mg/ml, the inhibitory effect of ISMN liposomes was the same as that of ISMN (P>0.05). At 45 and 23 mg/ml, the inhibitory effect of ISMN immunoliposomes on formed biofilms was greater than that of ISMN liposomes and free ISMN (P<0.05) and the inhibitory effect of ISMN liposomes was stronger than that of free ISMN (P<0.05). At 11 mg/ml, ISMN immunoliposomes, ISMN liposomes, and ISMN had the same effect on formed biofilms (P>0.05). In conclusion, ISMN immunoliposomes nearly completely destroy biofilm structure. ISMN immunoliposomes provide a promising approach for treating infectious diseases caused by S. aureus biofilms, including refractory CRS, chronic skin infection, sepsis, and osteomyelitis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zhao Y, Dong D, Li X, Li Z, Li S and Wang J: Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms. Exp Ther Med 19: 1267-1274, 2020.
APA
Zhang, Y., Zhao, Y., Dong, D., Li, X., Li, Z., Li, S., & Wang, J. (2020). Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms. Experimental and Therapeutic Medicine, 19, 1267-1274. https://doi.org/10.3892/etm.2019.8344
MLA
Zhang, Y., Zhao, Y., Dong, D., Li, X., Li, Z., Li, S., Wang, J."Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms". Experimental and Therapeutic Medicine 19.2 (2020): 1267-1274.
Chicago
Zhang, Y., Zhao, Y., Dong, D., Li, X., Li, Z., Li, S., Wang, J."Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1267-1274. https://doi.org/10.3892/etm.2019.8344
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zhao Y, Dong D, Li X, Li Z, Li S and Wang J: Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms. Exp Ther Med 19: 1267-1274, 2020.
APA
Zhang, Y., Zhao, Y., Dong, D., Li, X., Li, Z., Li, S., & Wang, J. (2020). Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms. Experimental and Therapeutic Medicine, 19, 1267-1274. https://doi.org/10.3892/etm.2019.8344
MLA
Zhang, Y., Zhao, Y., Dong, D., Li, X., Li, Z., Li, S., Wang, J."Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms". Experimental and Therapeutic Medicine 19.2 (2020): 1267-1274.
Chicago
Zhang, Y., Zhao, Y., Dong, D., Li, X., Li, Z., Li, S., Wang, J."Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms". Experimental and Therapeutic Medicine 19, no. 2 (2020): 1267-1274. https://doi.org/10.3892/etm.2019.8344
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