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Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway

  • Authors:
    • Chao Zhu
    • Na Na
    • Haibo Sheng
    • Bing Feng
    • Hao Wang
    • Ping Zhu
    • Wei Zhang
    • Meina Zhang
    • Zhen Deng
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Department of Outpatients, 900th Hospital of The Joint Logistics Support Force (People's Liberation Army), Fuzhou, Fujian 350000, P.R. China, Department of Urology, Airforce Medical Center (People's Liberation Army), Beijing 100142, P.R. China, Department of Pathology, 971st Navy Hospital of PLA, Qingdao, Shandong 266071, P.R. China, Department of Urology, 900th Hospital of The Joint Logistics Support Force (People's Liberation Army), Fuzhou, Fujian 350000, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2949-2956
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    Published online on: February 27, 2020
       https://doi.org/10.3892/etm.2020.8570
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Abstract

Renal cell carcinoma (RCC) is one of the most common urological malignancies occurring in adult human kidneys worldwide. Recent research on antitumor drugs has focused on plant extracts, a class of compounds that play critical roles in cancer treatment. The present study aimed to investigate the potential antitumor effect of ginkgolic acid (GA) in RCC. Transwell invasion assay, cell counting kit‑8 assay and flow cytometry were used to measure cell migration, cell viability and apoptosis, respectively. A network pharmacology approach was applied to identify pathway information, combining molecular docking techniques to screen for key target information. In the present study, GA inhibited the viability and proliferation of RCC cells (786‑O and A498), both in vitro and in vivo, via G1 arrest. GA also reduced RCC cell invasion and migration. In addition, the epidermal growth factor receptor (EGFR) was identified as a critical target protein of GA, which significantly inactivated EGFR signaling in RCC (P<0.05). Collectively, the present study provided evidence that GA exerts its anticancer function by directly targeting the EGFR signaling pathway, revealing the potential of GA therapy for RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu C, Na N, Sheng H, Feng B, Wang H, Zhu P, Zhang W, Zhang M and Deng Z: Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway. Exp Ther Med 19: 2949-2956, 2020.
APA
Zhu, C., Na, N., Sheng, H., Feng, B., Wang, H., Zhu, P. ... Deng, Z. (2020). Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway. Experimental and Therapeutic Medicine, 19, 2949-2956. https://doi.org/10.3892/etm.2020.8570
MLA
Zhu, C., Na, N., Sheng, H., Feng, B., Wang, H., Zhu, P., Zhang, W., Zhang, M., Deng, Z."Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway". Experimental and Therapeutic Medicine 19.4 (2020): 2949-2956.
Chicago
Zhu, C., Na, N., Sheng, H., Feng, B., Wang, H., Zhu, P., Zhang, W., Zhang, M., Deng, Z."Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2949-2956. https://doi.org/10.3892/etm.2020.8570
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu C, Na N, Sheng H, Feng B, Wang H, Zhu P, Zhang W, Zhang M and Deng Z: Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway. Exp Ther Med 19: 2949-2956, 2020.
APA
Zhu, C., Na, N., Sheng, H., Feng, B., Wang, H., Zhu, P. ... Deng, Z. (2020). Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway. Experimental and Therapeutic Medicine, 19, 2949-2956. https://doi.org/10.3892/etm.2020.8570
MLA
Zhu, C., Na, N., Sheng, H., Feng, B., Wang, H., Zhu, P., Zhang, W., Zhang, M., Deng, Z."Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway". Experimental and Therapeutic Medicine 19.4 (2020): 2949-2956.
Chicago
Zhu, C., Na, N., Sheng, H., Feng, B., Wang, H., Zhu, P., Zhang, W., Zhang, M., Deng, Z."Ginkgolic acid inhibits the growth of renal cell carcinoma cells via inactivation of the EGFR signaling pathway". Experimental and Therapeutic Medicine 19, no. 4 (2020): 2949-2956. https://doi.org/10.3892/etm.2020.8570
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