Open Access

Paxillin promotes the migration and angiogenesis of HUVECs and affects angiogenesis in the mouse cornea

  • Authors:
    • Wan‑Ju Yang
    • Jiang‑Bo Yan
    • Li Zhang
    • Fang Zhao
    • Zhong‑Ming Mei
    • Yan‑Ning Yang
    • Yi Xiang
    • Yi‑Qiao Xing
  • View Affiliations

  • Published online on: May 14, 2020     https://doi.org/10.3892/etm.2020.8751
  • Pages: 901-909
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Neonatal vascular ophthalmopathy is a refractory ophthalmologic disease, and is a major cause of blindness. Occurrence of neonatal vascular ophthalmopathy may be associated with Paxillin, a cellular adhesion molecule which promotes the migration of endothelial cells and angiogenesis. To explore the role of PXN in corneal angiogenesis, human umbilical vein endothelial cells were divided into five groups: i) Control group; ii) Empty vector‑transfected control group; iii) PXN knockdown group (shPXN group); iv) PXN‑negative control (NC) group; and v) PXN over‑expressed group (overExp group). PXN protein levels, migration and tube formation were assessed in the different experimental groups. Mice were divided into four groups: i) Control; ii) Model; iii) shPXN; and iv) overExp groups. Tube formation was significantly increased in the overExp group compared with the empty vector‑transfected control group (P<0.01). Tube formation was significantly decreased in the shPXN group compared with the PXN‑NC group (P<0.01). In mice, blood corpuscles were significantly decreased in the shPXN group. PXN promoted the migration of endothelial cells and corneal angiogenesis. The results of the present study suggest a role for PXN in corneal angiogenesis and provide a theoretical basis and potential target for the treatment of corneal angiogenesis.

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August-2020
Volume 20 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Yang, W., Yan, J., Zhang, L., Zhao, F., Mei, Z., Yang, Y. ... Xing, Y. (2020). Paxillin promotes the migration and angiogenesis of HUVECs and affects angiogenesis in the mouse cornea. Experimental and Therapeutic Medicine, 20, 901-909. https://doi.org/10.3892/etm.2020.8751
MLA
Yang, W., Yan, J., Zhang, L., Zhao, F., Mei, Z., Yang, Y., Xiang, Y., Xing, Y."Paxillin promotes the migration and angiogenesis of HUVECs and affects angiogenesis in the mouse cornea". Experimental and Therapeutic Medicine 20.2 (2020): 901-909.
Chicago
Yang, W., Yan, J., Zhang, L., Zhao, F., Mei, Z., Yang, Y., Xiang, Y., Xing, Y."Paxillin promotes the migration and angiogenesis of HUVECs and affects angiogenesis in the mouse cornea". Experimental and Therapeutic Medicine 20, no. 2 (2020): 901-909. https://doi.org/10.3892/etm.2020.8751