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TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors

  • Authors:
    • Wenchao Han
    • Xiumin Chen
    • Xianyin Wang
    • Zhen Shen
    • Xiaobing Wang
    • Zuofeng Zhang
    • Huiru Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Puyang Hospital of Traditional Chinese Medicine, Puyang, Henan 457000, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1291-1298
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    Published online on: May 30, 2020
       https://doi.org/10.3892/etm.2020.8825
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Abstract

Expression and diagnostic value of serum toll‑like receptor‑4 (TLR‑4), Toll‑like receptor‑5 (TLR‑5) and interferon regulatory factor 4 (IRF4) in middle‑aged and elderly patients with knee osteoarthritis (KOA) and their correlation with Interleukin 1β (IL‑1β), interleukin‑6 (IL‑6), matrix metalloproteinase‑1 and tumor necrosis factor‑α (TNF‑α) were investigated. Sixty‑eight middle‑aged and elderly patients with KOA in Puyang Hospital of Traditional Chinese Medicine were selected as the study group and 49 healthy people receiving physical examination were the control group. Levels of serum TLR‑4, TLR‑5, IRF4, IL‑1β, IL‑6, MMP‑1 and TNF‑α were measured by enzyme linked immunosorbent assay (ELISA). Correlation between the expression levels of serum TLR‑4, TLR‑5, IRF4 and K‑L grades was determined by Spearman correlation analysis. The diagnostic efficacy of serum TLR‑4, TLR‑5 and IRF4 for KOA was analyzed by the receiver operator characteristics analysis (ROC). Expression of serum TLR‑4, TLR‑5 and IRF4 in the study group was significantly higher than those in the control group. The sensitivity and specificity of TLR‑4 in the diagnosis of KOA were, respectively, 76.47 and 93.88%, those of TLR‑5 were 73.29 and 87.76%, those of IRF4 were 72.06 and 95.92%, and those of TLR‑4, TLR‑5 and IRF4 were 94.12 and 97.96%. Expression of serum TLR‑4, TLR‑5 and IRF4 was significantly higher in the severe group than in the moderate group, and significantly higher in the moderate group than those the in mild group, and significantly higher in the mild group than those in the suspected mild group. Expression of TLR‑4, TLR‑5 and IRF4 in serum was positively correlated with the concentration of IL‑1β, IL‑6, MMP‑1 and TNF‑α, respectively (P<0.001). The combined detection of TLR‑4, TLR‑5 and IRF4 can be used for early diagnosis of KOA, and they are positively correlated with IL‑1β and IL‑6, MMP‑1 and TNF‑α.
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1 

Chen H, Zheng X, Huang H, Liu C, Wan Q and Shang S: The effects of a home-based exercise intervention on elderly patients with knee osteoarthritis: A quasi-experimental study. BMC Musculoskelet Disord. 20(160)2019.PubMed/NCBI View Article : Google Scholar

2 

Peixoto JG, de Souza Moreira B, Diz JBM, Timoteo EF, Kirkwood RN and Teixeira-Salmela LF: Analysis of symmetry between lower limbs during gait of older women with bilateral knee osteoarthritis. Aging Clin Exp Res. 31:67–73. 2019.PubMed/NCBI View Article : Google Scholar

3 

Gigis I, Fotiadis E, Nenopoulos A, Tsitas K and Hatzokos I: Comparison of two different molecular weight intra-articular injections of hyaluronic acid for the treatment of knee osteoarthritis. Hippokratia. 20:26–31. 2016.PubMed/NCBI

4 

Clynes MA, Jameson KA, Edwards MH, Cooper C and Dennison EM: Impact of osteoarthritis on activities of daily living: Does joint site matter? Aging Clin Exp Res. 31:1049–1056. 2019.PubMed/NCBI View Article : Google Scholar

5 

Paik J, Duggan ST and Keam SJ: Correction to: Triamcinolone aceonide extended-release: A review in osteoarthritis pain of the knee. Drugs. 79(691)2019.PubMed/NCBI View Article : Google Scholar

6 

Akintayo RO, Yerima A, Olaosebikan HB, Uhunmwangho C and Akpabio AA: How much gloom is in groans? Depression and its determinants in Nigerian patients with knee osteoarthritis: A multi-center cross-sectional study. Clin Rheumatol. 38:1971–1978. 2019.PubMed/NCBI View Article : Google Scholar

7 

Camacho Encina M, Calamia V, Picchi Figueira F, Van Duine J, Qiu J, Ruiz Romero C, LaBaer J and Blanco F: Discovery of an autoantibody profile for the early diagnosis of knee osteoarthritis using NAPPA: Data from the osteoarthritis initiative. Osteoarthritis Cartilage. 26:S39–S40. 2018.

8 

Ryan MD, Roulston C, de Felipe P, Odon V, Tilsner J and Luke GA: The potential consequences for cell signaling by a class of NOD-like receptor proteins (NLRs) bearing an N-terminal signal sequence. J Cell Signal. 2(148)2017.

9 

Kandahari AM, Yang X, Dighe AS, Pan D and Cui Q: Recognition of immune response for the early diagnosis and treatment of osteoarthritis. J Immunol Res. 2015(192415)2015.PubMed/NCBI View Article : Google Scholar

10 

Xu WD, Pan HF, Ye DQ and Xu Y: Targeting IRF4 in autoimmune diseases. Autoimmun Rev. 11:918–924. 2012.PubMed/NCBI View Article : Google Scholar

11 

Schoenemeyer A, Barnes BJ, Mancl ME, Latz E, Goutagny N, Pitha PM, Fitzgerald KA and Golenbock DT: The interferon regulatory factor, IRF5, is a central mediator of toll-like receptor 7 signaling. J Biol Chem. 280:17005–17012. 2005.PubMed/NCBI View Article : Google Scholar

12 

Petrasek J, Dolganiuc A, Csak T, Kurt-Jones EA and Szabo G: Type I interferons protect from Toll-like receptor 9-associated liver injury and regulate IL-1 receptor antagonist in mice. Gastroenterology. 140:697–708.e4. 2011.PubMed/NCBI View Article : Google Scholar

13 

Mullen LM, Chamberlain G and Sacre S: Pattern recognition receptors as potential therapeutic targets in inflammatory rheumatic disease. Arthritis Res Ther. 17(122)2015.PubMed/NCBI View Article : Google Scholar

14 

Jiang W, Wang H, Li YS and Luo W: Role of vasoactive intestinal peptide in osteoarthritis. J Biomed Sci. 23(63)2016.PubMed/NCBI View Article : Google Scholar

15 

Nair A, Kanda V, Bush-Joseph C, Verma N, Chubinskaya S, Mikecz K, Glant TT, Malfait AM, Crow MK, Spear GT, et al: Synovial fluid from patients with early osteoarthritis modulates fibroblast-like synoviocyte responses to toll-like receptor 4 and toll-like receptor 2 ligands via soluble CD14. Arthritis Rheum. 64:2268–2277. 2012.PubMed/NCBI View Article : Google Scholar

16 

Chamberlain ND, Vila OM, Volin MV, Volkov S, Pope RM, Swedler W, Mandelin AM II and Shahrara S: TLR5, a novel and unidentified inflammatory mediator in rheumatoid arthritis that correlates with disease activity score and joint TNF-α levels. J Immunol. 189:475–483. 2012.PubMed/NCBI View Article : Google Scholar

17 

Yang C, He D, Yin C and Tan J: Inhibition of interferon regulatory factor 4 suppresses Th1 and Th17 cell differentiation and ameliorates experimental autoimmune encephalomyelitis. Scand J Immunol. 82:345–351. 2015.PubMed/NCBI View Article : Google Scholar

18 

Chang MW, Cook AD, Nutt SL, Hamilton JA and Lacey DC: Interferon regulatory factor 4 and inflammation. Cytokine. 70(33)2014.

19 

Lana JF, Macedo A, Ingrao ILG, Huber SC, Santos GS and Santana MHA: Leukocyte-rich PRP for knee osteoarthritis: Current concepts. J Clin Orthop Trauma. 10 (Suppl 1):S179–S182. 2019.PubMed/NCBI View Article : Google Scholar

20 

Rogoveanu OC, Calina D, Cucu MG, Burada F, Docea AO, Sosoi S, Stefan E, Ioana M and Burada E: Association of cytokine gene polymorphisms with osteoarthritis susceptibility. Exp Ther Med. 16:2659–2664. 2018.PubMed/NCBI View Article : Google Scholar

21 

Zhang R, Yang X, Wang J, Han L, Yang A, Zhang J, Zhang D, Li B, Li Z and Xiong Y: Identification of potential biomarkers for differential diagnosis between rheumatoid arthritis and osteoarthritis via integrative genome-wide gene expression profiling analysis. Mol Med Rep. 19:30–40. 2019.PubMed/NCBI View Article : Google Scholar

22 

Trachana V, Mourmoura E, Papathanasiou I and Tsezou A: Understanding the role of chondrocytes in osteoarthritis: Utilizing proteomics. Expert Rev Proteomics. 16:201–213. 2019.PubMed/NCBI View Article : Google Scholar

23 

Hochberg MC, Altman RD, Brandt KD, Clark BM, Dieppe PA, Griffin MR, Moskowitz RW and Schnitzer TJ: American College of Rheumatology: Guidelines for the medical management of osteoarthritis. Part I. Osteoarthritis of the hip. Arthritis Rheum. 38:1535–1540. 1995.PubMed/NCBI View Article : Google Scholar

24 

Kraus VB, Catterall J, Soderblom E, Moseley MA and Suchindran S: Development of a serum biomarker panel highly predictive of knee osteoarthritis progression. Osteoarthritis Cartilage. 24(S20)2016.

25 

Brandt KD, Fife RS, Braunstein EM and Katz B: Radiographic grading of the severity of knee osteoarthritis: Relation of the Kellgren and Lawrence grade to a grade based on joint space narrowing, and correlation with arthroscopic evidence of articular cartilage degeneration. Arthritis Rheum. 34:1381–1386. 1991.PubMed/NCBI View Article : Google Scholar

26 

Norman B, Pedoia V, Noworolski A, Link TM and Majumdar S: Applying densely connected convolutional neural networks for staging osteoarthritis severity from plain radiographs. J Digit Imaging. 32:471–477. 2019.PubMed/NCBI View Article : Google Scholar

27 

Ahmed U, Anwar A, Savage RS, Thornalley PJ and Rabbani N: Protein oxidation, nitration and glycation biomarkers for early-stage diagnosis of osteoarthritis of the knee and typing and progression of arthritic disease. Arthritis Res Ther. 18(250)2016.PubMed/NCBI View Article : Google Scholar

28 

Chen D, Shen J, Zhao W, Wang T, Han L, Hamilton JL and Im HJ: Osteoarthritis: Toward a comprehensive understanding of pathological mechanism. Bone Res. 5(16044)2017.PubMed/NCBI View Article : Google Scholar

29 

Liang H, Wang H, Luo L, Fan S, Zhou L, Liu Z, Yao S, Zhang X, Zhong K, Zhao H and Zha Z: Toll-like receptor 4 promotes high glucose-induced catabolic and inflammatory responses in chondrocytes in an NF-κB-dependent manner. Life Sci. 228:258–265. 2019.PubMed/NCBI View Article : Google Scholar

30 

Jin J, Yu X, Hu Z, Tang S, Zhong X, Xu J, Shang P, Huang Y and Liu H: Isofraxidin targets the TLR4/MD-2 axis to prevent osteoarthritis development. Food Funct. 9:5641–5652. 2018.PubMed/NCBI View Article : Google Scholar

31 

Mudter J, Yu J, Zufferey C, Brüstle A, Wirtz S, Weigmann B, Hoffman A, Schenk M, Galle PR, Lehr HA, et al: IRF4 regulates IL-17A promoter activity and controls RORγ t-dependent Th17 colitis in vivo. Inflamm Bowel Dis. 17:1343–1358. 2011.PubMed/NCBI View Article : Google Scholar

32 

Brüstle A, Heink S, Huber M, Rosenplänter C, Stadelmann C, Yu P, Arpaia E, Mak TW, Kamradt T and Lohoff M: The development of inflammatory T(H)-17 cells requires interferon-regulatory factor 4. Nat Immunol. 8:958–966. 2007.PubMed/NCBI View Article : Google Scholar

33 

Lim R, Barker G and Lappas M: The TLR2 ligand FSL-1 and the TLR5 ligand Flagellin mediate pro-inflammatory and pro-labour response via MyD88/TRAF6/NF-κB-dependent signalling. Am J Reprod Immunol. 71:401–417. 2014.PubMed/NCBI View Article : Google Scholar

34 

Kwa MQ, Nguyen T, Huynh J, Ramnath D, De Nardo D, Lam PY, Reynolds EC, Hamilton JA, Sweet MJ and Scholz GM: Interferon regulatory factor 6 differentially regulates Toll-like receptor 2-dependent chemokine gene expression in epithelial cells. J Biol Chem. 289:19758–19768. 2014.PubMed/NCBI View Article : Google Scholar

35 

Miller RE, Scanzello CR and Malfait AM: An emerging role for Toll-like receptors at the neuroimmune interface in osteoarthritis. Semin Immunopathol. 41:583–594. 2019.PubMed/NCBI View Article : Google Scholar

36 

Ospelt C, Brentano F, Rengel Y, Stanczyk J, Kolling C, Tak PP, Gay RE, Gay S and Kyburz D: Overexpression of toll-like receptors 3 and 4 in synovial tissue from patients with early rheumatoid arthritis: Toll-like receptor expression in early and longstanding arthritis. Arthritis Rheum. 58:3684–3692. 2008.PubMed/NCBI View Article : Google Scholar

37 

Lee MC, Saleh R, Achuthan A, Fleetwood AJ, Förster I, Hamilton JA and Cook AD: CCL17 blockade as a therapy for osteoarthritis pain and disease. Arthritis Res Ther. 20(62)2018.PubMed/NCBI View Article : Google Scholar

38 

Ding L, Hong X, Sun B, Huang Q, Wang X, Liu X, Li L, Huang Z and Liu D: IL-37 is associated with osteoarthritis disease activity and suppresses proinflammatory cytokines production in synovial cells. Sci Rep. 7(11601)2017.PubMed/NCBI View Article : Google Scholar

39 

Haseeb A, Ansari MY and Haqqi TM: Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes. J Orthop Res. 35:311–320. 2017.PubMed/NCBI View Article : Google Scholar

40 

Zhang C, Wang P, Jiang P, Lv Y, Dong C, Dai X, Tan L and Wang Z: Upregulation of lncRNA HOTAIR contributes to IL-1β-induced MMP overexpression and chondrocytes apoptosis in temporomandibular joint osteoarthritis. Gene. 586:248–253. 2016.PubMed/NCBI View Article : Google Scholar

41 

Li ZC, Han N, Li X, Li G, Liu YZ, Sun GX, Wang Y, Chen GT and Li GF: Decreased expression of microRNA-130a correlates with TNF-α in the development of osteoarthritis. Int J Clin Exp Pathol. 8:2555–2564. 2015.PubMed/NCBI

42 

Zheng W, Zhang H, Jin Y, Wang Q, Chen L, Feng Z, Chen H and Wu Y: Butein inhibits IL-1β-induced inflammatory response in human osteoarthritis chondrocytes and slows the progression of osteoarthritis in mice. Int Immunopharmacol. 42:1–10. 2017.PubMed/NCBI View Article : Google Scholar

43 

Zeng GQ, Chen AB, Li W, Song JH and Gao CY: High MMP-1, MMP-2, and MMP-9 protein levels in osteoarthritis. Genet Mol Res. 14:14811–14822. 2015.PubMed/NCBI View Article : Google Scholar

44 

Stannus O, Jones G, Cicuttini F, Parameswaran V, Quinn S, Burgess J and Ding C: Circulating levels of IL-6 and TNF-α are associated with knee radiographic osteoarthritis and knee cartilage loss in older adults. Osteoarthritis Cartilage. 18:1441–1447. 2010.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Han W, Chen X, Wang X, Shen Z, Wang X, Zhang Z and Wang H: TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors . Exp Ther Med 20: 1291-1298, 2020.
APA
Han, W., Chen, X., Wang, X., Shen, Z., Wang, X., Zhang, Z., & Wang, H. (2020). TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors . Experimental and Therapeutic Medicine, 20, 1291-1298. https://doi.org/10.3892/etm.2020.8825
MLA
Han, W., Chen, X., Wang, X., Shen, Z., Wang, X., Zhang, Z., Wang, H."TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors ". Experimental and Therapeutic Medicine 20.2 (2020): 1291-1298.
Chicago
Han, W., Chen, X., Wang, X., Shen, Z., Wang, X., Zhang, Z., Wang, H."TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors ". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1291-1298. https://doi.org/10.3892/etm.2020.8825
Copy and paste a formatted citation
x
Spandidos Publications style
Han W, Chen X, Wang X, Shen Z, Wang X, Zhang Z and Wang H: TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors . Exp Ther Med 20: 1291-1298, 2020.
APA
Han, W., Chen, X., Wang, X., Shen, Z., Wang, X., Zhang, Z., & Wang, H. (2020). TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors . Experimental and Therapeutic Medicine, 20, 1291-1298. https://doi.org/10.3892/etm.2020.8825
MLA
Han, W., Chen, X., Wang, X., Shen, Z., Wang, X., Zhang, Z., Wang, H."TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors ". Experimental and Therapeutic Medicine 20.2 (2020): 1291-1298.
Chicago
Han, W., Chen, X., Wang, X., Shen, Z., Wang, X., Zhang, Z., Wang, H."TLR‑4, TLR‑5 and IRF4 are diagnostic markers of knee osteoarthritis in the middle-aged and elderly patients and related to disease activity and inflammatory factors ". Experimental and Therapeutic Medicine 20, no. 2 (2020): 1291-1298. https://doi.org/10.3892/etm.2020.8825
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